Detailed view for Tb927.3.4850

Basic information

TDR Targets ID: 18641
Trypanosoma brucei, enoyl-CoA hydratase, mitochondrial precursor, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.287 | Length (AA): 267 | MW (Da): 28605 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00378   Enoyl-CoA hydratase/isomerase

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 264 2uzf (A) 6 266 33.00 0 1 1.33458 0.16
11 267 1mj3 (A) 31 290 50.00 0 1 1.57635 -0.47
12 266 3h81 (A) 23 277 49.00 0 1 1.59586 -0.74
16 266 2pbp (A) 7 257 45.00 0 1 1.53147 -0.64
16 205 4fn7 (A) 5 194 27.00 0.00000061 1 1.11601 -0.94

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128562)

Species Accession Gene Product
Brugia malayi Bm1_30520   Probable enoyl-CoA hydratase, mitochondrial
Caenorhabditis elegans CELE_Y105E8A.4   Protein ECH-7
Caenorhabditis elegans CELE_T05G5.6   Protein ECH-6
Dictyostelium discoideum DDB_G0285071   enoyl Coenzyme A hydratase, mitochondrial
Drosophila melanogaster Dmel_CG6543   CG6543 gene product from transcript CG6543-RA
Escherichia coli b1393   2,3-dehydroadipyl-CoA hydratase
Echinococcus granulosus EgrG_000702200   enoyl coenzyme A hydratase
Echinococcus multilocularis EmuJ_000702200   enoyl coenzyme A hydratase
Homo sapiens ENSG00000127884   enoyl CoA hydratase, short chain, 1, mitochondrial
Leishmania braziliensis LbrM.29.2280   enoyl-CoA hydratase/isomerase-like protein
Leishmania donovani LdBPK_292420.1   enoyl-CoA hydratase/isomerase-like protein
Leishmania infantum LinJ.29.2420   enoyl-CoA hydratase/isomerase-like protein
Leishmania major LmjF.29.2310   enoyl-CoA hydratase/isomerase-like protein
Leishmania mexicana LmxM.08_29.2310   enoyl-CoA hydratase/isomerase-like protein
Loa Loa (eye worm) LOAG_10253   hypothetical protein
Mycobacterium leprae ML2402   PROBABLE ENOYL-CoA HYDRATASE ECHA8 (ENOYL HYDRASE) (UNSATURATED ACYL-CoA HYDRATASE) (CROTONASE)
Mus musculus ENSMUSG00000025465   enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Mycobacterium tuberculosis Rv1070c   Probable enoyl-CoA hydratase EchA8 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase)
Mycobacterium ulcerans MUL_3889   enoyl-CoA hydratase EchA16
Mycobacterium ulcerans MUL_0126   enoyl-CoA hydratase, EchA8
Mycobacterium ulcerans MUL_2758   enoyl-CoA hydratase, EchA8_5
Mycobacterium ulcerans MUL_0210   enoyl-CoA hydratase
Mycobacterium ulcerans MUL_0310   enoyl-CoA hydratase, EchA8_2
Mycobacterium ulcerans MUL_3888   enoyl-CoA hydratase EchA11
Neospora caninum NCLIV_012140   enoyl-CoA hydratase/isomerase family domain- containing protein, putative
Schmidtea mediterranea mk4.000573.00  
Trypanosoma brucei gambiense Tbg972.3.5430   enoyl-CoA hydratase, mitochondrial precursor, putative
Trypanosoma brucei Tb927.3.4850   enoyl-CoA hydratase, mitochondrial precursor, putative
Trypanosoma congolense TcIL3000_0_42580   enoyl-CoA hydratase, mitochondrial precursor, putative
Trypanosoma cruzi TcCLB.508153.130   enoyl-CoA hydratase, mitochondrial precursor, putative
Trypanosoma cruzi TcCLB.508185.10   enoyl-CoA hydratase, mitochondrial precursor, putative
Toxoplasma gondii TGME49_317705   enoyl-CoA hydratase/isomerase family protein

Essentiality

Tb927.3.4850 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1087 Mycobacterium tuberculosis non-essential nmpdr
Tb927.3.4850 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.3.4850 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.4850 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.4850 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b1393 Escherichia coli non-essential goodall
CELE_T05G5.6 Caenorhabditis elegans larval arrest wormbase
CELE_T05G5.6 Caenorhabditis elegans slow growth wormbase
TGME49_317705 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Crotonase (4.2.1.17 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.3.4850 (Trypanosoma brucei), enoyl-CoA hydratase, mitochondrial precursor, putative
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