Detailed view for Tb927.11.5480

Basic information

TDR Targets ID: 18663
Trypanosoma brucei, phosphotransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.2676 | Length (AA): 540 | MW (Da): 60775 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00929   Exonuclease
PF01885   RNA 2'-phosphotransferase, Tpt1 / KptA family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016772   transferase activity, transferring phosphorus-containing groups  
GO:0006388   tRNA splicing  
GO:0005622   intracellular  
GO:0004527   exonuclease activity  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 275 4qoz (E) 102 349 34.00 0.0000000098 1 0.756604 -0.52
16 275 1zbu (B) 107 349 38.00 0 1 0.875982 -0.42
22 237 2xri (A) 134 334 38.00 0 1 0.8925 -1.07
339 534 1wfx (A) 3 181 26.00 0 0.92 0.562463 0.18

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127645)

Species Accession Gene Product
Arabidopsis thaliana AT2G45330   2' tRNA phosphotransferase
Arabidopsis thaliana AT5G23600   tRNA 2'phosphotransferase
Brugia malayi Bm1_05475   exonuclease family protein
Candida albicans CaO19.5432   likely tRNA 2'-phosphotransferase similar to S. cerevisiae TPT1 (YOL102C) responsible for the last step of tRNA splicing
Candida albicans CaO19.12887   likely tRNA 2'-phosphotransferase similar to S. cerevisiae TPT1 (YOL102C) responsible for the last step of tRNA splicing
Caenorhabditis elegans CELE_M02B7.2   Protein M02B7.2
Caenorhabditis elegans CELE_R02D3.8   Protein R02D3.8
Cryptosporidium hominis Chro.20133   CG33057-PA
Cryptosporidium parvum cgd2_1210   conserved hypothetical protein
Dictyostelium discoideum DDB_G0286929   hypothetical protein
Drosophila melanogaster Dmel_CG33057   CG33057 gene product from transcript CG33057-RB
Escherichia coli b4331   RNA 2'-phosphotransferase
Echinococcus granulosus EgrG_000057100   Exonuclease RNase T DNA polymerase III
Echinococcus multilocularis EmuJ_000057100   Exonuclease, RNase T DNA polymerase III
Homo sapiens ENSG00000117419   ERI1 exoribonuclease family member 3
Homo sapiens ENSG00000149743   tRNA phosphotransferase 1
Leishmania braziliensis LbrM.24.0830   phosphotransferase, putative
Leishmania donovani LdBPK_240830.1   phosphotransferase, putative
Leishmania infantum LinJ.24.0830   phosphotransferase, putative
Leishmania major LmjF.24.0810   phosphotransferase, putative
Leishmania mexicana LmxM.24.0810   phosphotransferase, putative
Loa Loa (eye worm) LOAG_10927   exonuclease
Mus musculus ENSMUSG00000047656   tRNA phosphotransferase 1
Mus musculus ENSMUSG00000033423   exoribonuclease 3
Neospora caninum NCLIV_032600   tRNA splicing 2 phosphotransferase 1, putative
Neospora caninum NCLIV_029770   TRNA splicing 2 phosphotransferase 1, related
Oryza sativa 4326611   Os01g0618000
Oryza sativa 4350620   Os11g0525900
Onchocerca volvulus OVOC3906   ERI1 exoribonuclease 3 homolog
Saccharomyces cerevisiae YOL102C   Tpt1p
Schistosoma mansoni Smp_157760   prion interactor pint1
Schistosoma mansoni Smp_142480   tRNA splicing 2' phosphotransferase 1
Schmidtea mediterranea mk4.007803.00  
Trypanosoma brucei gambiense Tbg972.11.6190   phosphotransferase, putative
Trypanosoma brucei Tb927.11.5480   phosphotransferase, putative
Trypanosoma congolense TcIL3000.11.5760   phosphotransferase, putative
Trypanosoma cruzi TcCLB.508647.250   phosphotransferase, putative
Toxoplasma gondii TGME49_257290   RNA 2'-phosphotransferase, Tpt1/KptA family protein
Toxoplasma gondii TGME49_232640   RNA 2'-phosphotransferase, Tpt1/KptA family protein

Essentiality

Tb927.11.5480 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.3160 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.3160 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.3160 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.02.3160 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b4331 Escherichia coli non-essential goodall
YOL102C Saccharomyces cerevisiae inviable yeastgenome
TGME49_257290 Toxoplasma gondii Probably essential sidik
TGME49_232640 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Candida albicans likely tRNA 2'-phosphotransferase similar to S. cerevisiae TPT1 (YOL102C) responsible for the last step of tRNA splicing Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.5480 (Trypanosoma brucei), phosphotransferase, putative
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