Detailed view for Tb927.11.1260

Basic information

TDR Targets ID: 18719
Trypanosoma brucei, copper-transporting ATPase-like protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1903 | Length (AA): 961 | MW (Da): 102336 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 8

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00122   E1-E2 ATPase
PF00403   Heavy-metal-associated domain
PF00702   haloacid dehalogenase-like hydrolase

Gene Ontology

Mouse over links to read term descriptions.
GO:0019829   cation-transporting ATPase activity  
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005524   ATP binding  
GO:0005375   copper ion transmembrane transporter activity  
GO:0004008   copper-exporting ATPase activity  
GO:0003824   catalytic activity  
GO:0000166   nucleotide binding  
GO:0046873   metal ion transmembrane transporter activity  
GO:0046872   metal ion binding  
GO:0006812   cation transport  
GO:0006754   ATP biosynthetic process  
GO:0030001   metal ion transport  
GO:0008152   metabolic process  
GO:0006825   copper ion transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
99 167 1aw0 (A) 2 70 30.00 0.0036 1 0.5936 -1.33
103 165 4a4j (A) 6 67 39.00 0.0034 1 0.621357 -0.87
105 174 1kvi (A) 13 79 31.00 0.00063 1 0.416641 0.15
106 168 1yjr (A) 10 72 29.00 0.0048 0.96 0.479357 -0.39
180 254 1kvi (A) 4 78 19.00 0 0.92 0.357144 -0.31
183 254 3dxs (X) 1 72 22.00 0 0.97 0.528022 -1.7
187 253 2kt2 (A) 3 68 35.00 0 1 0.564819 -0.94
271 947 4bbj (A) 75 732 33.00 0 1 1.01457 0.31
406 517 2hc8 (A) 214 325 43.00 0 1 0.731645 -1.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126855)

Species Accession Gene Product
Arabidopsis thaliana AT1G63440   putative copper-transporting ATPase HMA5
Arabidopsis thaliana AT5G44790   copper-transporting ATPase RAN1
Brugia malayi Bm1_00795   E1-E2 ATPase family protein
Candida albicans CaO19.4328   copper-transporting ATPase
Candida albicans CaO19.11803   copper-transporting ATPase
Caenorhabditis elegans CELE_Y76A2A.2   Protein CUA-1, isoform B
Cryptosporidium hominis Chro.30424   P-type ATPase 2
Cryptosporidium parvum cgd3_3740   P-type ATPase 2
Dictyostelium discoideum DDB_G0273235   P-type ATPase
Dictyostelium discoideum DDB_G0284141   P-type ATPase
Dictyostelium discoideum DDB_G0273675   P-type ATPase
Drosophila melanogaster Dmel_CG1886   CG1886 gene product from transcript CG1886-RC
Escherichia coli b0484   copper transporter
Echinococcus granulosus EgrG_001195000   copper transporting ATPase 1
Echinococcus multilocularis EmuJ_001195000   copper transporting ATPase 1
Homo sapiens ENSG00000123191   ATPase, Cu++ transporting, beta polypeptide
Homo sapiens ENSG00000165240   ATPase, Cu++ transporting, alpha polypeptide
Leishmania braziliensis LbrM.33.2370   copper-transporting ATPase-like protein, putative
Leishmania donovani LdBPK_332210.1   copper-transporting ATPase-like protein, putative
Leishmania infantum LinJ.33.2210   copper-transporting ATPase-like protein, putative
Leishmania major LmjF.33.2090   copper-transporting ATPase-like protein, putative
Leishmania mexicana LmxM.32.2090   copper-transporting ATPase-like protein, putative
Loa Loa (eye worm) LOAG_09008   hypothetical protein
Loa Loa (eye worm) LOAG_01704   hypothetical protein
Mycobacterium leprae ML2000c   PROBABLE CATION-TRANSPORTER P-TYPE ATPASE B CTPB
Mycobacterium leprae ML1987   PROBABLE CATION TRANSPORTER P-TYPE ATPASE A CTPA
Mus musculus 11977   ATPase, Cu++ transporting, alpha polypeptide
Mus musculus ENSMUSG00000006567   ATPase, Cu++ transporting, beta polypeptide
Mycobacterium tuberculosis Rv0103c   Probable cation-transporter P-type ATPase B CtpB
Mycobacterium tuberculosis Rv0092   Cation transporter P-type ATPase a CtpA
Mycobacterium tuberculosis Rv0969   Probable metal cation transporter P-type ATPase CtpV
Mycobacterium ulcerans MUL_4845   cation-transporter p-type ATPase B CtpB
Mycobacterium ulcerans MUL_4850   cation transporter p-type ATPase a CtpA
Mycobacterium ulcerans MUL_0423   metal cation transporter p-type ATPase, CtpV
Neospora caninum NCLIV_023240   Heavy metal translocating P-type ATPase, related
Oryza sativa 4328616   Os02g0196600
Oryza sativa 4341778   Os06g0665800
Oryza sativa 4336625   Os04g0556000
Oryza sativa 4328448   Os02g0172600
Plasmodium berghei PBANKA_0416500   copper-transporting ATPase
Plasmodium falciparum PF3D7_0904900   copper-transporting ATPase
Plasmodium knowlesi PKNH_0702600   copper-transporting ATPase, putative
Saccharomyces cerevisiae YDR270W   Cu(2+)-transporting P-type ATPase CCC2
Schistosoma japonicum Sjp_0000890   Copper-transporting ATPase 2, putative
Schistosoma japonicum Sjp_0000900   Copper-transporting ATPase 2, putative
Schistosoma japonicum Sjp_0000920   ko:K01533 Cu2+-exporting ATPase [EC3.6.3.4], putative
Schistosoma mansoni Smp_144970   copper ABC transporter ATPase
Schmidtea mediterranea mk4.000189.02  
Schmidtea mediterranea mk4.003571.02  
Schmidtea mediterranea mk4.002977.02  
Schmidtea mediterranea mk4.003552.00   Atp7a protein
Schmidtea mediterranea mk4.000189.03   Atp7a protein
Schmidtea mediterranea mk4.000189.00  
Schmidtea mediterranea mk4.019004.00   Putative copper-transporting atpase 1, 2
Trypanosoma brucei gambiense Tbg972.11.1330   copper-transporting ATPase-like protein, putative
Trypanosoma brucei Tb927.11.1260   copper-transporting ATPase-like protein, putative
Trypanosoma congolense TcIL3000.11.1210   copper-transporting ATPase-like protein, putative
Trypanosoma cruzi TcCLB.509015.10   copper-transporting ATPase, putative
Trypanosoma cruzi TcCLB.511445.160   copper-transporting ATPase-like protein, putative
Trypanosoma cruzi TcCLB.503703.50   copper-transporting ATPase-like protein, putative
Trypanosoma cruzi TcCLB.503893.10   copper-transporting ATPase-like protein, putative
Toxoplasma gondii TGME49_201150   heavy metal translocating P-type ATPase subfamily protein
Treponema pallidum TP1036   cation-transporting ATPase, P-type

Essentiality

Tb927.11.1260 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu93 Mycobacterium tuberculosis non-essential nmpdr
mtu984 Mycobacterium tuberculosis non-essential nmpdr
Tb11.47.0023 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.47.0023 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.47.0023 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.47.0023 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0484 Escherichia coli non-essential goodall
CELE_Y76A2A.2 Caenorhabditis elegans adult lethal wormbase
CELE_Y76A2A.2 Caenorhabditis elegans larval arrest wormbase
CELE_Y76A2A.2 Caenorhabditis elegans larval lethal wormbase
CELE_Y76A2A.2 Caenorhabditis elegans slow growth wormbase
TGME49_201150 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Trypanosoma cruzi P-type H+-ATPase, putative 645 aa 22.2% 518 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.11.1260 (Trypanosoma brucei), copper-transporting ATPase-like protein, putative
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