pI: 6.6583 |
Length (AA): 535 |
MW (Da): 59456 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Modbase 3D models:
There are 3 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
---|---|---|---|---|---|---|---|---|---|
3 | 74 | 2v6z (M) | 30 | 99 | 16.00 | 0.0000005 | 0.59 | 0.434379 | -1.12 |
18 | 103 | 5fod (B) | 458 | 584 | 34.00 | 0.38 | 0.15 | 0.144148 | 1.79 |
75 | 517 | 5ihe (A) | 175 | 587 | 18.00 | 0 | 1 | 0.836837 | 0.5 |
Help me make sense of these data.
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
Upregulation Percent | Ranking | Stage | Dataset |
---|---|---|---|
Upper 60-80% percentile | Procyclic, Bloodstream Form. | Siegel TN |
Siegel TN | Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites. |
Ortholog group members (OG5_128285)
Species | Accession | Gene Product |
---|---|---|
Arabidopsis thaliana | AT5G22110 | DNA polymerase epsilon subunit B2 |
Brugia malayi | Bm1_34750 | DNA polymerase epsilon subunit B |
Brugia malayi | Bm1_34755 | pole2 protein |
Candida albicans | CaO19.7564 | similar to S. cerevisiae DPB2 (YPR175W) DNA polymerase epsilon subunit B |
Caenorhabditis elegans | CELE_F08B4.5 | Protein POLE-2 |
Cryptosporidium hominis | Chro.20265 | hypothetical protein |
Cryptosporidium parvum | cgd2_2500 | DNA polymerase epsilon subunit |
Dictyostelium discoideum | DDB_G0278367 | hypothetical protein |
Drosophila melanogaster | Dmel_CG10489 | CG10489 gene product from transcript CG10489-RA |
Echinococcus granulosus | EgrG_000414300 | dna polymerase epsilon subunit 2 |
Echinococcus multilocularis | EmuJ_000414300 | dna polymerase epsilon subunit 2 |
Giardia lamblia | GL50803_3706 | DNA pol epsilon, sub B |
Homo sapiens | ENSG00000100479 | polymerase (DNA directed), epsilon 2, accessory subunit |
Leishmania braziliensis | LbrM.34.1700 | DNA polymerase epsilon subunit b, putative |
Leishmania donovani | LdBPK_351780.1 | DNA polymerase epsilon subunit B, putative |
Leishmania infantum | LinJ.35.1780 | DNA polymerase epsilon subunit b, putative |
Leishmania major | LmjF.35.1790 | DNA polymerase epsilon subunit b, putative |
Leishmania mexicana | LmxM.34.1790 | DNA polymerase epsilon subunit b, putative |
Loa Loa (eye worm) | LOAG_01229 | pole2 protein |
Loa Loa (eye worm) | LOAG_14095 | hypothetical protein |
Loa Loa (eye worm) | LOAG_08035 | hypothetical protein |
Mus musculus | ENSMUSG00000020974 | polymerase (DNA directed), epsilon 2 (p59 subunit) |
Oryza sativa | 9272695 | Os08g0466050 |
Oryza sativa | 4337886 | Os05g0160800 |
Onchocerca volvulus | OVOC7346 | Putative DNA polymerase epsilon subunit 2 |
Onchocerca volvulus | OVOC7341 | Putative DNA polymerase epsilon subunit 2 |
Plasmodium berghei | PBANKA_1448900 | DNA polymerase epsilon subunit B, putative |
Plasmodium falciparum | PF3D7_1234300 | DNA polymerase epsilon subunit B, putative |
Plasmodium knowlesi | PKNH_1453700 | DNA polymerase epsilon subunit B, putative |
Plasmodium vivax | PVX_100665 | hypothetical protein, conserved |
Plasmodium yoelii | PY06200 | DNA polymerase epsilon subunit b |
Saccharomyces cerevisiae | YPR175W | Dpb2p |
Schistosoma japonicum | Sjp_0093770 | Conserved hypothetical protein |
Schistosoma japonicum | Sjp_0117470 | ko:K02325 DNA polymerase epsilon subunit 2, putative |
Schistosoma japonicum | Sjp_0000260 | ko:K02325 DNA polymerase epsilon subunit 2, putative |
Schistosoma mansoni | Smp_124120 | DNA polymerase epsilon subunit B |
Schmidtea mediterranea | mk4.000001.39 | |
Schmidtea mediterranea | mk4.003825.00 | Putative DNA polymerase epsilon subunit b |
Schmidtea mediterranea | mk4.005025.01 | Putative DNA polymerase epsilon subunit b |
Trypanosoma brucei gambiense | Tbg972.9.9290 | DNA polymerase epsilon subunit b, putative |
Trypanosoma brucei | Tb927.9.15030 | DNA polymerase epsilon subunit B, putative |
Trypanosoma congolense | TcIL3000_9_6250 | DNA polymerase epsilon subunit B, putative |
Trypanosoma cruzi | TcCLB.509671.160 | DNA polymerase epsilon subunit B, putative |
Trypanosoma cruzi | TcCLB.510763.80 | DNA polymerase epsilon subunit B, putative |
Trichomonas vaginalis | TVAG_030780 | DNA polymerase epsilon subunit B, putative |
Gene/Ortholog | Organism | Phenotype | Source Study |
---|---|---|---|
Tb09.244.2820 this record | Trypanosoma brucei | no significant loss or gain of fitness in bloodstream forms (3 days) | alsford |
Tb09.244.2820 this record | Trypanosoma brucei | significant gain of fitness in bloodstream forms (6 days) | alsford |
Tb09.244.2820 this record | Trypanosoma brucei | no significant loss or gain of fitness in procyclic forms | alsford |
Tb09.244.2820 this record | Trypanosoma brucei | no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms | alsford |
CELE_F08B4.5 | Caenorhabditis elegans | embryonic lethal | wormbase |
CELE_F08B4.5 | Caenorhabditis elegans | larval arrest | wormbase |
CELE_F08B4.5 | Caenorhabditis elegans | sterile | wormbase |
YPR175W | Saccharomyces cerevisiae | inviable | yeastgenome |
PBANKA_1448900 | Plasmodium berghei | Essential | plasmo |
nmpdr | Genome-scale essentiality datasets from published studies (M. tuberculosis) | National Microbial Pathogen Data Resource |
neb | C. elegans RNAi phenotypes | Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs |
gerdes | Experimental determination and system-level analysis of essential genes in E. coli MG1655 | Gerdes et al., J Bacteriol. 2003 185:5673-84 |
alsford | High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome | Genome Res 2011, 21:915-924 |
blattner | Systematic mutagenesis of the E. coli (MG1655) genome | J Bacteriol 2004, 186:4921-4930 |
shigen | Profiling of E. coli Chromosome (PEC) | National Institute of Genetics, Japan |
wormbase | C. elegans RNAi experiments | WormBase web site, http://www.wormbase.org, release WS170 |
keio | Systematic single-gene knock-out mutants of E. coli K12 | The Keio Collection |
yeastgenome | Systematic deletion of yeast genes | Saccharomyces Genome Database |
Affected Entity | Phenotypic quality | Occurs in | Occurs at | Evidence | Observed in | Drugs/Inhibitors |
---|---|---|---|---|---|---|
cell proliferation (GO:0008283) | normal (PATO:0000461) | bloodstream stage trypomastigotes (PLO:0027) | inferred from RNAi experiment (ECO:0000019) | No drug identifiers listed for this gene. | ||
Annotator: | fernan@iib.unsam.edu.ar. | Comment: | normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). | References: | 21363968 | |
cell proliferation (GO:0008283) | increased (PATO:0000470) | bloodstream stage trypomastigotes (PLO:0027) | inferred from RNAi experiment (ECO:0000019) | No drug identifiers listed for this gene. | ||
Annotator: | fernan@iib.unsam.edu.ar. | Comment: | increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). | References: | 21363968 | |
cell proliferation (GO:0008283) | normal (PATO:0000461) | procyclic (PLO:0034) | inferred from RNAi experiment (ECO:0000019) | No drug identifiers listed for this gene. | ||
Annotator: | fernan@iib.unsam.edu.ar. | Comment: | normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . | References: | 21363968 |
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
22 literature references were collected for this gene.