Detailed view for Tb927.11.7420

Basic information

TDR Targets ID: 18810
Trypanosoma brucei, phosphoadenosine phosphosulfate reductase-like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2395 | Length (AA): 322 | MW (Da): 36057 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01507   Phosphoadenosine phosphosulfate reductase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0016740   transferase activity  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 60 2jqd (A) 89 152 15.00 0.067 0.53 0.35773 -0.76
14 75 1fs2 (A) 336 399 16.00 0 0.17 0.419547 -0.97
86 320 3fwk (A) 4 301 24.00 0 1 0.892814 -0.48
215 319 2oq2 (A) 148 236 36.00 0.00019 0.38 0.406287 0.88

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128350)

Species Accession Gene Product
Arabidopsis thaliana AT5G03430   phosphoadenosine phosphosulfate (PAPS) reductase family protein
Babesia bovis BBOV_I002340   phosphoadenosine phosphosulfate reductase family domain containing protein
Brugia malayi Bm1_07710   Probable molybdopterin binding domain containing protein
Candida albicans CaO19.12186   Flavin adenine dinucleotide (FAD) synthetase
Candida albicans CaO19.4723   Flavin adenine dinucleotide (FAD) synthetase
Caenorhabditis elegans CELE_R53.1   Protein FLAD-1, isoform D
Dictyostelium discoideum DDB_G0282955   hypothetical protein
Drosophila melanogaster Dmel_CG16848   CG16848 gene product from transcript CG16848-RA
Drosophila melanogaster Dmel_CG4407   CG4407 gene product from transcript CG4407-RA
Echinococcus granulosus EgrG_000242200   fad synthase
Echinococcus multilocularis EmuJ_000242200   fad synthase
Giardia lamblia GL50803_16269   FAD synthetase
Homo sapiens ENSG00000160688   flavin adenine dinucleotide synthetase 1
Leishmania braziliensis LbrM.28.0280   phosphoadenosine phosphosulfate reductase-like protein
Leishmania donovani LdBPK_280410.1   phosphoadenosine phosphosulfate reductase-like protein
Leishmania infantum LinJ.28.0410   phosphoadenosine phosphosulfate reductase-like protein
Leishmania major LmjF.28.0270   phosphoadenosine phosphosulfate reductase-like protein
Leishmania mexicana LmxM.28.0270   phosphoadenosine phosphosulfate reductase-like protein
Loa Loa (eye worm) LOAG_11838   lenep protein
Mus musculus ENSMUSG00000042642   RFad1, flavin adenine dinucleotide synthetase, homolog (yeast)
Neospora caninum NCLIV_051520   DJ-1/PfpI family domian-containing protein, putative
Oryza sativa 4326240   Os01g0259600
Plasmodium berghei PBANKA_1213400   FAD synthetase, putative
Plasmodium falciparum PF3D7_1015000   FAD synthetase, putative
Plasmodium knowlesi PKNH_0815100   FAD synthetase, putative
Plasmodium vivax PVX_094970   FAD synthetase, putative
Plasmodium yoelii PY07439   fad synthetase
Saccharomyces cerevisiae YDL045C   FMN adenylyltransferase
Schistosoma japonicum Sjp_0301520   ko:K00953 FMN adenylyltransferase [EC2.7.7.2], putative
Schistosoma mansoni Smp_126650   molybdopterin-binding
Schmidtea mediterranea mk4.007983.02  
Trypanosoma brucei gambiense Tbg972.11.8490   phosphoadenosine phosphosulfate reductase-like protein, putative
Trypanosoma brucei Tb927.11.7420   phosphoadenosine phosphosulfate reductase-like protein
Trypanosoma congolense TcIL3000.11.8020   phosphoadenosine phosphosulfate reductase-like protein
Trypanosoma congolense TcIL3000_0_31180   phosphoadenosine phosphosulfate reductase-like protein
Trypanosoma cruzi TcCLB.511151.50   phosphoadenosine phosphosulfate reductase-like protein, putative
Trypanosoma cruzi TcCLB.508241.60   phosphoadenosine phosphosulfate reductase-like protein, putative
Toxoplasma gondii TGME49_214280   phosphoadenosine phosphosulfate reductase family protein
Theileria parva TP03_0087   FAD synthetase, putative
Trichomonas vaginalis TVAG_486660   fad synthetase, putative
Trichomonas vaginalis TVAG_322610   molybdopterin- binding protein, putative
Trichomonas vaginalis TVAG_166700   fad synthetase, putative

Essentiality

Tb927.11.7420 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.5320 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.5320 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.5320 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.5320 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_R53.1 Caenorhabditis elegans slow growth wormbase
YDL045C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1213400 Plasmodium berghei Dispensable plasmo
TGME49_214280 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Hepatitis B virus Core antigen Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.11.7420 (Trypanosoma brucei), phosphoadenosine phosphosulfate reductase-like protein
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