Detailed view for Tb927.9.9860

Basic information

TDR Targets ID: 18816
Trypanosoma brucei, 170 kDa glucose-regulated protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.4949 | Length (AA): 720 | MW (Da): 79858 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00012   Hsp70 protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0003674   molecular_function  
GO:0005524   ATP binding  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128359)

Species Accession Gene Product
Arabidopsis thaliana AT4G16660   heat shock protein 70
Babesia bovis BBOV_I003640   DnaK family domain containing protein
Brugia malayi Bm1_39345   dnaK protein
Candida albicans CaO19.5830   translocation of protein precursors across ER
Candida albicans CaO19.13252   translocation of protein precursors across ER
Caenorhabditis elegans CELE_T14G8.3   Protein T14G8.3, isoform A
Caenorhabditis elegans CELE_T24H7.2   Protein T24H7.2
Cryptosporidium hominis Chro.20349   HSP protein
Cryptosporidium parvum cgd2_3330   APG-1 like HSP70 domain containing protein, signal peptide plus likely ER retention motif
Dictyostelium discoideum DDB_G0273093   heat shock protein 70 family member
Dictyostelium discoideum DDB_G0273813   heat shock protein 70 family member
Drosophila melanogaster Dmel_CG2918   CG2918 gene product from transcript CG2918-RB
Echinococcus granulosus EgrG_000981800   Heat shock protein Hsp70
Entamoeba histolytica EHI_148990   heat shock protein 70, putative
Echinococcus multilocularis EmuJ_000981800   Heat shock protein Hsp70
Homo sapiens ENSG00000149428   hypoxia up-regulated 1
Leishmania braziliensis LbrM.34.4680   hypothetical protein, conserved
Leishmania donovani LdBPK_354780.1   Hsp70 protein, putative
Leishmania infantum LinJ.35.4780   hypothetical protein, conserved
Leishmania major LmjF.35.4710   hypothetical protein, conserved
Leishmania mexicana LmxM.34.4710   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_02797   dnaK protein
Mus musculus ENSMUSG00000032115   hypoxia up-regulated 1
Neospora caninum NCLIV_046170   Heat Shock Protein 70, ER lumen, related
Oryza sativa 4330492   Os02g0710900
Plasmodium berghei PBANKA_1357200   heat shock protein 110, putative
Plasmodium falciparum PF3D7_1344200   heat shock protein 110, putative
Plasmodium knowlesi PKNH_1257200   heat shock protein 110, putative
Plasmodium vivax PVX_083105   heat shock protein 110, putative
Plasmodium yoelii PY05402   putative HSP protein
Saccharomyces cerevisiae YKL073W   Hsp70 family chaperone LHS1
Schistosoma japonicum Sjp_0060680   ko:K09486 hypoxia up-regulated 1, putative
Schistosoma mansoni Smp_088950   hypothetical protein
Schmidtea mediterranea mk4.000709.08   Hypoxia up-regulated protein 1
Schmidtea mediterranea mk4.000785.07   Hypoxia up-regulated protein 1
Trypanosoma brucei gambiense Tbg972.9.5670   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.9860   170 kDa glucose-regulated protein, putative
Trypanosoma cruzi TcCLB.508457.20   Hsp70 protein, putative
Trypanosoma cruzi TcCLB.506885.440   Hsp70 protein, putative
Toxoplasma gondii TGME49_226830   DnaK family protein
Theileria parva TP01_0479   dnaK domain protein, putative

Essentiality

Tb927.9.9860 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.1390 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.1390 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.1390 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.1390 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1357200 Plasmodium berghei Essential plasmo
TGME49_226830 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bos taurus Heat shock cognate 71 kDa protein 650 aa 23.8% 685 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.9.9860 (Trypanosoma brucei), 170 kDa glucose-regulated protein, putative
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