Detailed view for Tb927.11.10970

Basic information

TDR Targets ID: 18862
Trypanosoma brucei, RWD domain/Uncharacterized protein family UPF0029, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.9465 | Length (AA): 260 | MW (Da): 29056 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01205   Uncharacterized protein family UPF0029

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129517)

Species Accession Gene Product
Candida albicans CaO19.3273   similar to S. cerevisiae YCR059C involved in regulation of amino acid metabolsm
Candida albicans CaO19.3274   general amino acid control pathway
Candida albicans CaO19.10784   general amino acid control pathway
Candida albicans CaO19.10783   similar to S. cerevisiae YCR059C involved in regulation of amino acid metabolsm
Caenorhabditis elegans CELE_Y52B11A.2   Protein Y52B11A.2, isoform B
Cryptosporidium parvum cgd2_2430   ximpact ortholog conserved protein seen in bacteria and eukaryotes
Dictyostelium discoideum DDB_G0287757   hypothetical protein
Echinococcus granulosus EgrG_000983600   protein IMPACT
Entamoeba histolytica EHI_013300   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000983600   protein IMPACT
Giardia lamblia GL50803_15984   Impact-like protein
Homo sapiens ENSG00000154059   impact RWD domain protein
Leishmania braziliensis LbrM.35.5190   hypothetical protein, conserved
Leishmania donovani LdBPK_365170.1   Uncharacterized protein family UPF0029, putative
Leishmania infantum LinJ.36.5170   hypothetical protein, conserved
Leishmania major LmjF.36.4940   hypothetical protein, conserved
Leishmania mexicana LmxM.36.4940   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_15784   hypothetical protein
Loa Loa (eye worm) LOAG_14972   hypothetical protein
Mus musculus ENSMUSG00000024423   impact, RWD domain protein
Onchocerca volvulus OVOC3400  
Saccharomyces cerevisiae YCR059C   Yih1p
Schistosoma japonicum Sjp_0135010   IPR001498,Protein of unknown function UPF0029;IPR006575,RWD,domain-containing
Schistosoma japonicum Sjp_0210280   Protein IMPACT-A, putative
Schistosoma japonicum Sjp_0057040   IPR001498,Protein of unknown function UPF0029;IPR006575,RWD,domain-containing
Schistosoma japonicum Sjp_0119610   IPR006575,RWD,domain-containing
Schistosoma mansoni Smp_029370   hypothetical protein
Schmidtea mediterranea mk4.006384.00   Protein IMPACT homolog
Trypanosoma brucei gambiense Tbg972.11.12300   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.10970   RWD domain/Uncharacterized protein family UPF0029, putative
Trypanosoma congolense TcIL3000.11.11690   RWD domain/Uncharacterized protein family UPF0029, putative
Trypanosoma cruzi TcCLB.508269.10   RWD domain/Uncharacterized protein family UPF0029, putative
Trypanosoma cruzi TcCLB.503971.20   RWD domain/Uncharacterized protein family UPF0029, putative
Trichomonas vaginalis TVAG_291410   impact, putative

Essentiality

Tb927.11.10970 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.2750 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.2750 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.2750 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.2750 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.10970 (Trypanosoma brucei), RWD domain/Uncharacterized protein family UPF0029, putative
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