Detailed view for Tb927.5.2940

Basic information

TDR Targets ID: 18896
Trypanosoma brucei, stress-induced protein sti1, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2235 | Length (AA): 550 | MW (Da): 62328 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00515   Tetratricopeptide repeat
PF07720   Tetratricopeptide repeat
PF13181   Tetratricopeptide repeat
PF13414   TPR repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 116 1elw (A) 3 117 46.00 0 1 0.881091 -1.47
225 507 2ifu (A) 6 264 17.00 0 1 0.581545 0.28
485 550 2llw (A) 524 589 50.00 0.000000000019 1 0.9028 -1.91

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128289)

Species Accession Gene Product
Arabidopsis thaliana AT1G62740   carboxylate clamp-tetratricopeptide repeat protein HOP2
Arabidopsis thaliana AT4G12400   carboxylate clamp-tetratricopeptide repeat protein
Arabidopsis thaliana AT1G12270   protein HOP1
Babesia bovis BBOV_III002230   tetratricopeptide repeat domain containing protein
Brugia malayi Bm1_55560   TPR Domain
Candida albicans CaO19.3191   co-chaperonin STI1-like orf, has 9 TPR repeats
Candida albicans CaO19.10702   co-chaperonin STI1-like orf, has 9 TPR repeats
Candida albicans CaO19.3192   see CaP19.3191
Caenorhabditis elegans CELE_R09E12.3   Protein STI-1
Cryptosporidium hominis Chro.20201   stress-induced protein sti1-like protein
Cryptosporidium parvum cgd2_1850   stress-induced protein sti1-like protein, putative
Dictyostelium discoideum DDB_G0292404   stress-induced-phosphoprotein 1
Drosophila melanogaster Dmel_CG2720   Hsp70/Hsp90 organizing protein homolog
Echinococcus granulosus EgrG_000264900   stress induced phosphoprotein 1
Entamoeba histolytica EHI_023300   TPR repeat protein
Echinococcus multilocularis EmuJ_000264900   stress induced phosphoprotein 1
Giardia lamblia GL50803_27310   Stress-induced-phosphoprotein 1
Homo sapiens ENSG00000168439   stress-induced phosphoprotein 1
Leishmania braziliensis LbrM.08.0880   stress-induced protein sti1
Leishmania donovani LdBPK_081020.1   stress-induced protein sti1
Leishmania infantum LinJ.08.1020   stress-induced protein sti1
Leishmania major LmjF.08.1110   stress-induced protein sti1
Leishmania mexicana LmxM.08.1110   stress-induced protein sti1
Loa Loa (eye worm) LOAG_08731   TPR domain-containing protein
Mus musculus ENSMUSG00000024966   stress-induced phosphoprotein 1
Neospora caninum NCLIV_007330   Similar to uniprot
Oryza sativa 4336524   Os04g0538000
Oryza sativa 4330134   Os02g0644100
Plasmodium berghei PBANKA_1010500   Hsp70/Hsp90 organizing protein, putative
Plasmodium falciparum PF3D7_1434300   Hsp70/Hsp90 organizing protein
Plasmodium knowlesi PKNH_0420900   Hsp70/Hsp90 organizing protein, putative
Plasmodium vivax PVX_084820   Hsp70/Hsp90 organizing protein, putative
Plasmodium yoelii PY03138   stress-induced protein sti1-like protein
Saccharomyces cerevisiae YOR027W   Hsp90 cochaperone STI1
Schistosoma japonicum Sjp_0217290   ko:K09553 stress-induced-phosphoprotein 1, putative
Schistosoma mansoni Smp_064860   heat shock protein 70
Schmidtea mediterranea mk4.025349.00  
Schmidtea mediterranea mk4.000342.01   Stress-induced-phosphoprotein 1
Trypanosoma brucei gambiense Tbg972.5.4130   stress-induced protein sti1, putative
Trypanosoma brucei Tb927.5.2940   stress-induced protein sti1, putative
Trypanosoma congolense TcIL3000_5_2850   stress-induced protein sti1, putative
Trypanosoma congolense TcIL3000_5_3210   stress-induced protein sti1, putative
Trypanosoma cruzi TcCLB.506321.290   stress-induced protein sti1, putative
Toxoplasma gondii TGME49_252220   tetratricopeptide repeat domain containing protein
Theileria parva TP03_0587   hypothetical protein, conserved
Trichomonas vaginalis TVAG_220990   chaperone binding protein, putative
Trichomonas vaginalis TVAG_069560   O-linked N-acetylglucosamine transferase, ogt, putative

Essentiality

Tb927.5.2940 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.2940 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.2940 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.2940 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.2940 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_R09E12.3 Caenorhabditis elegans sterile wormbase
PBANKA_1010500 Plasmodium berghei Dispensable plasmo
TGME49_252220 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.5.2940 (Trypanosoma brucei), stress-induced protein sti1, putative
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