Detailed view for PF3D7_1141600

Basic information

TDR Targets ID: 1895
Plasmodium falciparum, dolichol-phosphate mannosyltransferase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.6784 | Length (AA): 259 | MW (Da): 30101 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00535   Glycosyl transferase family 2

Gene Ontology

Mouse over links to read term descriptions.
GO:0004582   dolichyl-phosphate beta-D-mannosyltransferase activity  
GO:0005783   endoplasmic reticulum  
GO:0006486   protein amino acid glycosylation  

Metabolic Pathways

N-Glycan biosynthesis (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
19 259 1xhb (A) 111 383 16.00 0 0.59 1.11 -0.62
27 259 2bo4 (A) 3 244 12.00 0 0.99 1.06 -1.19
24 237 1xhb (A) 116 360 15.00 0.00000051 0.98 0.976955 0.01
24 245 2z86 (B) 432 642 17.00 0 0.94 1.03484 0.2
25 153 3bcv (A) 6 149 31.00 0.00037 1 0.85367 -0.57
26 237 3e25 (A) 45 265 19.00 0.0000014 1 1.02423 -0.12
27 132 2z86 (A) 153 254 22.00 0.0057 0.59 0.736866 -0.9

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 32 hs, late trophozoite. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, early trophozoite, late ring, late schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, Oocyst, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, gametocyte. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Sporozoite, Female Gametocyte, Male Gametocyte. Zanghi G Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127079)

Species Accession Gene Product
Arabidopsis thaliana AT1G20575   dolichol-phosphate mannosyltransferase
Babesia bovis BBOV_III011210   glycosyl transferase, group 2 family protein
Brugia malayi Bm1_48770   dolichol monophosphate mannose synthase
Candida albicans CaO19.12539   likely glycosyltransferase similar to S. cerevisiae DPM1 (YPR183W) ER dolichol phosphate mannose synthase
Candida albicans CaO19.5073   likely glycosyltransferase similar to S. cerevisiae DPM1 (YPR183W) ER dolichol phosphate mannose synthase
Caenorhabditis elegans CELE_Y66H1A.2   Protein DPM-1
Cryptosporidium hominis Chro.50175   dolichyl-phosphate b-D-mannosyltransferase
Cryptosporidium parvum cgd5_2040   dolichol phosphate mannose synthase, putative
Dictyostelium discoideum DDB_G0286519   dolichol-phosphate-mannose synthase
Drosophila melanogaster Dmel_CG10166   CG10166 gene product from transcript CG10166-RA
Escherichia coli b2254   undecaprenyl phosphate-L-Ara4FN transferase
Escherichia coli b2351   CPS-53 (KpLE1) prophage
Echinococcus granulosus EgrG_000843600   dolichol phosphate mannosyltransferase
Entamoeba histolytica EHI_054260   dolichol monophosphate mannose synthase, putative
Echinococcus multilocularis EmuJ_000843600   dolichol phosphate mannosyltransferase
Giardia lamblia GL50803_3180   Dolichol-phosphate mannosyltransferase, putative
Homo sapiens ENSG00000000419   dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
Leishmania braziliensis LbrM.35.0300   dolicholphosphate-mannose synthase
Leishmania donovani LdBPK_360240.1   dolicholphosphate-mannose synthase
Leishmania infantum LinJ.36.0240   dolicholphosphate-mannose synthase
Leishmania major LmjF.36.0220   dolicholphosphate-mannose synthase
Leishmania mexicana LmxM.36.0220   dolichyl-phosphate beta-D-mannosyltransferase precursor, conserved
Loa Loa (eye worm) LOAG_01500   dolichol Phosphate Mannosyltransferase family member
Mycobacterium leprae ML1440c   Polyprenol-monophosphomannose synthase Ppm1a
Mus musculus ENSMUSG00000078919   dolichol-phosphate (beta-D) mannosyltransferase 1
Mycobacterium tuberculosis Rv1500   Probable glycosyltransferase
Mycobacterium tuberculosis Rv2051c   Polyprenol-monophosphomannose synthase Ppm1
Mycobacterium ulcerans MUL_4169   polyprenol-monophosphomannose synthase Ppm1_2
Mycobacterium ulcerans MUL_2269   C-term polyprenol-monophosphomannose synthase Ppm1B
Mycobacterium ulcerans MUL_5127   N-term polyprenol-monophosphomannose synthase Ppm1A
Neospora caninum NCLIV_068040   hypothetical protein
Oryza sativa 4334626   Os03g0824400
Plasmodium berghei PBANKA_0907400   dolichol-phosphate mannosyltransferase, putative
Plasmodium falciparum PF3D7_1141600   dolichol-phosphate mannosyltransferase
Plasmodium knowlesi PKNH_0939400   dolichol-phosphate mannosyltransferase, putative
Plasmodium vivax PVX_092685   dolichol-phosphate mannosyltransferase, putative
Plasmodium yoelii PY00944   dolichol phosphate mannose synthase
Saccharomyces cerevisiae YPR183W   dolichyl-phosphate beta-D-mannosyltransferase
Schistosoma japonicum Sjp_0211460   ko:K00721 dolichol-phosphate mannosyltransferase [EC2.4.1.83], putative
Schistosoma mansoni Smp_042790   dolichol-phosphate beta-D-mannosyltransferase
Schmidtea mediterranea mk4.040425.00  
Schmidtea mediterranea mk4.004497.01   Dolichol-phosphate beta-D-mannosyltransferase
Trypanosoma brucei gambiense Tbg972.10.5630   dolichyl-phosphate beta-D-mannosyltransferase precursor, putative
Trypanosoma brucei Tb927.10.4610   dolicholphosphate-mannose synthase, putative
Trypanosoma congolense TcIL3000_10_3820   dolichyl-phosphate beta-D-mannosyltransferase precursor, putative
Trypanosoma cruzi TcCLB.506581.10   dolicholphosphate-mannose synthase, putative
Trypanosoma cruzi TcCLB.508169.60   dolicholphosphate-mannose synthase, putative
Theileria parva TP02_0741   dolichol-phosphate mannosyltransferase, putative

Essentiality

PF3D7_1141600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1526 Mycobacterium tuberculosis non-essential nmpdr
Tb927.10.4610 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.4610 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.4610 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.4610 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2254 Escherichia coli non-essential goodall
b2351 Escherichia coli non-essential goodall
CELE_Y66H1A.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y66H1A.2 Caenorhabditis elegans larval arrest wormbase
YPR183W Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier PF3D7_1141600 (Plasmodium falciparum), dolichol-phosphate mannosyltransferase
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