Detailed view for Tb927.5.2640

Basic information

TDR Targets ID: 19002
Trypanosoma brucei, nicotinamide/nicotinic acid mononucleotide adenylyltransferase
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.5001 | Length (AA): 277 | MW (Da): 31245 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01467   Cytidylyltransferase-like

Gene Ontology

Mouse over links to read term descriptions.
GO:0016779   nucleotidyltransferase activity  
GO:0003824   catalytic activity  
GO:0009058   biosynthetic process  

Metabolic Pathways

Nicotinate and nicotinamide metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128044)

Species Accession Gene Product
Arabidopsis thaliana AT5G55810   bifunctional nicotinamide mononucleotide adenyltransferase and nicotinate-nucleotide adenylyltransferase
Brugia malayi Bm1_39780   Chain A, Crystal Structure Of NmnNAMN ADENYLYLTRANSFERASE COMPLEXED With Nad.
Brugia malayi Bm1_36205   Cytidylyltransferase family protein
Candida albicans CaO19.7499   similar to S. cerevisiae YLR328W
Caenorhabditis elegans CELE_F26H9.4   Protein NMAT-2
Caenorhabditis elegans CELE_W06B3.1   Protein NMAT-1
Dictyostelium discoideum DDB_G0288757   nicotinamide-nucleotide adenylyltransferase
Drosophila melanogaster Dmel_CG13645   Nicotinamide mononucleotide adenylyltransferase
Echinococcus granulosus EgrG_001062300   nicotinamide mononucleotide adenylyltransferase
Entamoeba histolytica EHI_084990   nicotinamide nucleotide adenylyltransferase, putative
Entamoeba histolytica EHI_010950   NMN adenylyltransferase, putative
Echinococcus multilocularis EmuJ_001062300   nicotinamide mononucleotide adenylyltransferase
Giardia lamblia GL50803_92618   Nicotinamide-nucleotide adenylyltransferase
Giardia lamblia GL50803_13672   Nicotinamide-nucleotide adenylyltransferase
Homo sapiens ENSG00000163864   nicotinamide nucleotide adenylyltransferase 3
Homo sapiens ENSG00000173614   nicotinamide nucleotide adenylyltransferase 1
Leishmania braziliensis LbrM.17.1470   hypothetical protein, conserved
Leishmania donovani LdBPK_171450.1   cytidylyltransferase, putative
Leishmania infantum LinJ.17.1450   hypothetical protein, conserved
Leishmania major LmjF.17.1340   hypothetical protein, conserved
Leishmania mexicana LmxM.17.1340   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_01131   cytidylyltransferase
Loa Loa (eye worm) LOAG_00727   hypothetical protein
Mus musculus ENSMUSG00000032456   nicotinamide nucleotide adenylyltransferase 3
Mus musculus ENSMUSG00000046402   retinol binding protein 1, cellular
Mus musculus ENSMUSG00000028992   nicotinamide nucleotide adenylyltransferase 1
Oryza sativa 4331124   Os02g0814900
Onchocerca volvulus OVOC535   Nicotinamide mononucleotide adenylyltransferase 2 homolog
Onchocerca volvulus OVOC5082   Nicotinamide mononucleotide adenylyltransferase 2 homolog
Saccharomyces cerevisiae YGR010W   nicotinamide-nucleotide adenylyltransferase NMA2
Saccharomyces cerevisiae YLR328W   nicotinamide-nucleotide adenylyltransferase NMA1
Schistosoma mansoni Smp_123490   nicotinamide mononucleotide adenylyltransferase
Schmidtea mediterranea mk4.008055.00   Nicotinamide mononucleotide adenylyltransferase 2
Trypanosoma brucei gambiense Tbg972.5.3720   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.2640   nicotinamide/nicotinic acid mononucleotide adenylyltransferase
Trypanosoma congolense TcIL3000_5_2680   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_5_2590   cytidylyltransferase, putative
Trypanosoma cruzi TcCLB.507047.170   cytidylyltransferase, putative
Trypanosoma cruzi TcCLB.509179.80   cytidylyltransferase, putative
Trichomonas vaginalis TVAG_110480   nicotinamide mononucleotide adenylyltransferase, putative
Trichomonas vaginalis TVAG_072070   nicotinamide mononucleotide adenylyltransferase, putative

Essentiality

Tb927.5.2640 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.2640 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.2640 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.2640 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.2640 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F26H9.4 Caenorhabditis elegans embryonic lethal wormbase
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.5.2640 (Trypanosoma brucei), nicotinamide/nicotinic acid mononucleotide adenylyltransferase
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