Detailed view for Tb927.6.4970

Basic information

TDR Targets ID: 19004
Trypanosoma brucei, serine/arginine-rich protein specific kinase SRPK, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.0803 | Length (AA): 723 | MW (Da): 82680 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
154 566 1q8y (A) 144 703 54.00 0 1 0.822431 0.13
156 589 1q8y (B) 146 728 39.00 0 1 0.694777 0.92
427 566 2b9h (A) 183 306 36.00 0.017 1 0.549838 -0.62
676 722 2bki (D) 49 111 13.00 0.18 0 0.162107 -0.15

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127107)

Species Accession Gene Product
Arabidopsis thaliana AT4G35500   protein kinase family protein
Arabidopsis thaliana AT5G22840   protein kinase family protein
Arabidopsis thaliana AT3G44850   serine/threonine kinase 23
Arabidopsis thaliana AT3G53030   ser/arg-rich protein kinase 4
Arabidopsis thaliana AT2G17530   putative protein kinase
Babesia bovis BBOV_III007370   protein kinase domain containing protein
Brugia malayi Bm1_44605   Protein kinase domain containing protein
Candida albicans CaO19.9972   one of two genes similar to S. cerevisiae SKY1 (YMR216C) which phosphorylaties mRNA shuttle protein Npl3p
Candida albicans CaO19.2436   one of two genes similar to S. cerevisiae SKY1 (YMR216C) which phosphorylaties mRNA shuttle protein Npl3p
Caenorhabditis elegans CELE_B0464.5   Protein SPK-1, isoform A
Cryptosporidium hominis Chro.10329   protein kinase domain
Cryptosporidium parvum cgd1_2960   protein kinase CMGC group, Sky1p like S/T protein kinase probably involved in RNA metabolism
Dictyostelium discoideum DDB_G0275627   SRPK family protein kinase
Drosophila melanogaster Dmel_CG8174   CG8174 gene product from transcript CG8174-RA
Drosophila melanogaster Dmel_CG11489   serine-arginine protein kinase at 79D
Echinococcus granulosus EgrG_000301500   expressed protein
Entamoeba histolytica EHI_104670   protein kinase, putative
Entamoeba histolytica EHI_035500   protein kinase, putative
Echinococcus multilocularis EmuJ_000301500   expressed protein
Giardia lamblia GL50803_17335   Kinase, CMGC SRPK
Homo sapiens ENSG00000096063   SRSF protein kinase 1
Homo sapiens ENSG00000184343   SRSF protein kinase 3
Homo sapiens ENSG00000135250   SRSF protein kinase 2
Leishmania braziliensis LbrM.30.3610   serine/threonine-protein kinase, putative,protein kinase, putative
Leishmania donovani LdBPK_303640.1   serine/threonine-protein kinase, putative
Leishmania infantum LinJ.30.3640   serine/threonine-protein kinase, putative,protein kinase, putative
Leishmania major LmjF.30.3580   serine/threonine-protein kinase, putative,protein kinase, putative
Leishmania mexicana LmxM.29.3580   serine/threonine-protein kinase, putative,protein kinase, putative
Loa Loa (eye worm) LOAG_03178   CMGC/SRPK protein kinase
Mus musculus 20815   serine/arginine-rich protein specific kinase 1
Mus musculus ENSMUSG00000002007   serine/arginine-rich protein specific kinase 3
Mus musculus ENSMUSG00000062604   serine/arginine-rich protein specific kinase 2
Neospora caninum NCLIV_007880   GM10776, related
Oryza sativa 4347794   Os09g0552300
Oryza sativa 4334107   Os03g0748400
Plasmodium berghei PBANKA_0401100   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_0302100   serine/threonine protein kinase
Plasmodium knowlesi PKNH_0840600   serine/threonine protein kinase, putative
Plasmodium vivax PVX_119250   serine/threonine protein kinase, putative
Plasmodium yoelii PY06573   Protein kinase domain, putative
Saccharomyces cerevisiae YMR216C   Sky1p
Schistosoma japonicum Sjp_0311460   expressed protein
Schistosoma japonicum Sjp_0000700   ko:K08831 SFRS protein kinase, putative
Schistosoma mansoni Smp_041770   serine/threonine protein kinase
Schmidtea mediterranea mk4.004082.01   Serine/threonine kinase
Schmidtea mediterranea mk4.004082.02  
Trypanosoma brucei gambiense Tbg972.6.4770   serine/arginine-rich protein specific kinase SRPK, putative,protein kinase, putative
Trypanosoma brucei Tb927.6.4970   serine/arginine-rich protein specific kinase SRPK, putative
Trypanosoma congolense TcIL3000_6_4400   serine/arginine-rich protein specific kinase SRPK, putative
Trypanosoma cruzi TcCLB.506945.220   serine/arginine-rich protein specific kinase SRPK, putative
Toxoplasma gondii TGME49_253440   cell-cycle-associated protein kinase SRPK, putative
Theileria parva TP04_0645   serine/threonine protein kinase, putative

Essentiality

Tb927.6.4970 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.4970 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.4970 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.4970 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.4970 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0464.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_B0464.5 Caenorhabditis elegans larval lethal wormbase
CELE_B0464.5 Caenorhabditis elegans sterile wormbase
PBANKA_0401100 Plasmodium berghei Slow plasmo
TGME49_253440 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens SRSF protein kinase 3 Compounds References
Homo sapiens SRSF protein kinase 1 Compounds References
Homo sapiens SRSF protein kinase 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0056 1 0.5
0.0059 1 1
0.0063 0.7244 0.2543
0.0091 1 0.5
0.0064 0.3377 0
0.0081 1 0.5
0.0022 0.5 0.5
0.0033 1 0.5
0.0093 0.8828 0
0.0063 1 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0012 0.5 0.5
0.0027 1 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.2614
0.0059 1 1
0.0039 0.9485 0.5
0.0039 0.5 0.5
0.0016 0.5 0.5
0.0032 0.5 0.5
0.0062 0.6935 0
0.0007 0.5 0.5
0.0061 0.6883 0.5304
0.0059 1 1
0.0008 0.5 0.5
0.0007 0.5 0.5
0.0067 0.5 0.5
0.0036 0.5 0.5
0.0003 0.5 0.5
0.0011 1 0.5
0.0033 0.5 0.5
0.0012 0.5 0.5
0.0081 0.5 0.5
0.0026 0.5 0.5
0.0066 0.3101 0
0.0018 0.5 0.5
0.0004 0.5 0.5
0.0092 1 0.5
0.0088 0.4477 0.5
0.0016 0.5 0.5
0.0037 1 0.5
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0042 0.5 0.5
0.0029 0.5 0.5
0.0069 0.3067 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.6.4970 (Trypanosoma brucei), serine/arginine-rich protein specific kinase SRPK, putative
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