Detailed view for Tb927.5.3220

Basic information

TDR Targets ID: 19027
Trypanosoma brucei, signal peptidase type I, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.861 | Length (AA): 208 | MW (Da): 23706 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00717   Peptidase S24-like

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0008233   peptidase activity  
GO:0006508   proteolysis  
GO:0006465   signal peptide processing  

Metabolic Pathways

Protein export (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
79 171 1jhc (A) 112 192 25.00 0 0.36 0.510815 0.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127356)

Species Accession Gene Product
Arabidopsis thaliana AT1G52600   peptidase S24/S26A/S26B/S26C family protein
Arabidopsis thaliana AT3G15710   peptidase S24/S26A/S26B/S26C family protein
Babesia bovis BBOV_III000270   signal peptidase, putative
Brugia malayi Bm1_48535   Microsomal signal peptidase 21 kDa subunit
Candida albicans CaO19.10769   signal peptidase subunit
Candida albicans CaO19.3259   signal peptidase subunit
Caenorhabditis elegans CELE_Y54E10BR.5   Protein Y54E10BR.5
Cryptosporidium parvum cgd1_440   hypothetical protein
Dictyostelium discoideum DDB_G0276359   microsomal signal peptidase subunit
Drosophila melanogaster Dmel_CG2358   twisted bristles roughened eye
Echinococcus granulosus EgrG_000341800   signal peptidase complex catalytic subunit
Entamoeba histolytica EHI_197020   signal peptidase, putative
Echinococcus multilocularis EmuJ_000341800   signal peptidase complex catalytic subunit
Giardia lamblia GL50803_9174   Microsomal signal peptidase 18 kDa subunit
Homo sapiens ENSG00000140612   SEC11 homolog A (S. cerevisiae)
Homo sapiens ENSG00000166562   SEC11 homolog C (S. cerevisiae)
Leishmania donovani LdBPK_080460.1   signal peptidase type I, putative
Leishmania infantum LinJ.08.0460   signal peptidase type I, putative
Leishmania major LmjF.08.0450   signal peptidase type I, putative
Leishmania mexicana LmxM.08.0450   signal peptidase type I, putative,serine peptidase, Clan SF, Family S26A
Loa Loa (eye worm) LOAG_03449   microsomal signal peptidase 21 kDa subunit
Mus musculus ENSMUSG00000025724   SEC11 homolog A (S. cerevisiae)
Mus musculus ENSMUSG00000024516   SEC11 homolog C (S. cerevisiae)
Neospora caninum NCLIV_019450   hypothetical protein
Oryza sativa 4338334   Os05g0297900
Oryza sativa 4340738   Os06g0273800
Oryza sativa 4331226   Os02g0827900
Onchocerca volvulus OVOC1066  
Plasmodium berghei PBANKA_1346200   signal peptidase 21 kDa subunit, putative
Plasmodium falciparum PF3D7_1331300   signal peptidase 21 kDa subunit
Plasmodium knowlesi PKNH_1269200   signal peptidase 21 kDa subunit, putative
Plasmodium vivax PVX_082500   signal peptidase 21 kDa subunit, putative
Plasmodium yoelii PY00480   signal peptidase 18 subunit-related
Saccharomyces cerevisiae YIR022W   Sec11p
Schistosoma japonicum Sjp_0058200   ko:K03100 signal peptidase I [EC3.4.21.89], putative
Schistosoma mansoni Smp_031730   signalase (S26 family)
Schmidtea mediterranea mk4.000058.06   GM04682p
Trypanosoma brucei gambiense Tbg972.5.4490   signal peptidase type I, putative,serine peptidase, Clan SF, Family S26A
Trypanosoma brucei Tb927.5.3220   signal peptidase type I, putative
Trypanosoma congolense TcIL3000_0_11990   signal peptidase type I, putative
Trypanosoma cruzi TcCLB.511661.40   signal peptidase type I, putative
Trypanosoma cruzi TcCLB.507809.74   signal peptidase type I, putative
Toxoplasma gondii TGME49_280740   signal peptidase
Theileria parva TP03_0804   signal peptidase, putative
Trichomonas vaginalis TVAG_240820   Clan SF, family S26, signal peptidase I-like serine peptidase

Essentiality

Tb927.5.3220 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.3220 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.3220 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.3220 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.3220 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y54E10BR.5 Caenorhabditis elegans larval lethal wormbase
CELE_Y54E10BR.5 Caenorhabditis elegans slow growth wormbase
CELE_Y54E10BR.5 Caenorhabditis elegans sterile wormbase
YIR022W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1346200 Plasmodium berghei Essential plasmo
TGME49_280740 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.5.3220 (Trypanosoma brucei), signal peptidase type I, putative
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