Detailed view for Tb927.11.3300

Basic information

TDR Targets ID: 19068
Trypanosoma brucei, Spindle assembly abnormal 4
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.2156 | Length (AA): 932 | MW (Da): 107861 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07202   T-complex protein 10 C-terminus

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130432)

Species Accession Gene Product
Caenorhabditis elegans CELE_F52C9.8   Protein PQE-1, isoform A
Drosophila melanogaster Dmel_CG10061   Spindle assembly abnormal 4 ortholog (C. elegans)
Echinococcus granulosus EgrG_000800000   centromere protein j
Echinococcus multilocularis EmuJ_000800000   centromere protein j
Giardia lamblia GL50803_13352   T-complex protein-10
Homo sapiens ENSG00000151849   centromere protein J
Leishmania braziliensis LbrM.13.1450   hypothetical protein, conserved
Leishmania donovani LdBPK_131330.1   Spindle assembly abnormal 4
Leishmania infantum LinJ.13.1330   hypothetical protein, conserved
Leishmania major LmjF.13.1590   hypothetical protein, conserved
Leishmania mexicana LmxM.13.1590   hypothetical protein, conserved
Mus musculus ENSMUSG00000064128   centromere protein J
Schistosoma japonicum Sjp_0023400   Centromere protein J, putative
Schistosoma mansoni Smp_180020   lyst-interacting protein
Schmidtea mediterranea mk4.002572.02   Centromere protein J
Trypanosoma brucei gambiense Tbg972.11.3690   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.3300   Spindle assembly abnormal 4
Trypanosoma congolense TcIL3000.11.3110   Spindle assembly abnormal 4
Trypanosoma cruzi TcCLB.509589.50   Spindle assembly abnormal 4
Trypanosoma cruzi TcCLB.503999.40   Spindle assembly abnormal 4
Trichomonas vaginalis TVAG_348400   t complex protein, putative
Trichomonas vaginalis TVAG_159630   lyst-interacting protein, putative
Trichomonas vaginalis TVAG_018390   lyst-interacting protein, putative

Essentiality

Tb927.11.3300 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.0810 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.0810 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.0810 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.0810 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.11.3300 (Trypanosoma brucei), Spindle assembly abnormal 4
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