Detailed view for Tb927.8.3860

Basic information

TDR Targets ID: 19189
Trypanosoma brucei, cytosolic iron-sulfur protein assembly 1
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.5628 | Length (AA): 424 | MW (Da): 46945 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0003674   molecular_function  
GO:0097361   GO:CIA complex  

GO:0005515   protein binding  
GO:0016226   iron-sulfur cluster assembly  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127906)

Species Accession Gene Product
Arabidopsis thaliana AT4G32990   WD40 domain-containing protein
Arabidopsis thaliana AT2G26060   cytosolic iron-sulfur protein assembly protein CIA1
Babesia bovis BBOV_III001050   WD domain, G-beta repeat containing protein
Brugia malayi Bm1_22720   hypothetical protein
Candida albicans CaO19.11769   similar to proteins with WD (WD-40) repeats
Candida albicans CaO19.4293   similar to proteins with WD (WD-40) repeats
Caenorhabditis elegans CELE_Y18D10A.9   Protein Y18D10A.9
Cryptosporidium hominis Chro.10254   WD40 protein Ciao1
Cryptosporidium parvum cgd1_2230   WD40 protein Ciao1
Dictyostelium discoideum DDB_G0269728   WD40 repeat-containing protein
Drosophila melanogaster Dmel_CG12797   CG12797 gene product from transcript CG12797-RA
Echinococcus granulosus EgrG_000949100   cytosolic iron sulfur protein assembly
Entamoeba histolytica EHI_044480   WD domain containing protein
Echinococcus multilocularis EmuJ_000949100   cytosolic iron sulfur protein assembly
Giardia lamblia GL50803_17550   Transducin/WD-40 repeat protein family
Homo sapiens 9391   cytosolic iron-sulfur assembly component 1
Leishmania braziliensis LbrM.10.0110   hypothetical protein, conserved,WD-repeat containing protein
Leishmania donovani LdBPK_100090.1   hypothetical protein, conserved
Leishmania infantum LinJ.10.0090   hypothetical protein, conserved,WD-repeat containing protein
Leishmania major LmjF.10.0110   hypothetical protein, conserved,WD-repeat containing protein
Leishmania mexicana LmxM.10.0110   hypothetical protein, conserved,WD-repeat containing protein
Loa Loa (eye worm) LOAG_02246   WD repeat domain 39
Mus musculus ENSMUSG00000003662   cytosolic iron-sulfur protein assembly 1
Neospora caninum NCLIV_056580   hypothetical protein
Oryza sativa 4342847   Os07g0252000
Plasmodium berghei PBANKA_0607800   cytosolic iron-sulfur protein assembly protein 1, putative
Plasmodium falciparum PF3D7_1209400   cytosolic iron-sulfur protein assembly protein 1, putative
Plasmodium knowlesi PKNH_1309300   cytosolic iron-sulfur protein assembly protein 1, putative
Plasmodium vivax PVX_084500   cytosolic iron-sulfur protein assembly protein 1, putative
Plasmodium yoelii PY07576   WD40 protein Ciao1-related
Plasmodium yoelii PY00229   hypothetical protein
Saccharomyces cerevisiae YDR267C   Cia1p
Schistosoma japonicum Sjp_0108880   IPR001680,WD-40 repeat;IPR011046,WD40-like,domain-containing
Schistosoma japonicum Sjp_0210650   Protein CIAO1, putative
Schistosoma mansoni Smp_018400   hypothetical protein
Schmidtea mediterranea mk4.001822.00   Probable cytosolic iron-sulfur protein assembly protein CIAO1 homolog
Trypanosoma brucei gambiense Tbg972.8.3580   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.3860   cytosolic iron-sulfur protein assembly 1
Trypanosoma congolense TcIL3000_8_3740   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.503905.40   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507705.20   hypothetical protein, conserved
Toxoplasma gondii TGME49_313280   WD domain, G-beta repeat-containing protein
Theileria parva TP03_0735   hypothetical protein, conserved
Theileria parva TP03_0734   hypothetical protein
Trichomonas vaginalis TVAG_350560   WD repeat domain, putative
Trichomonas vaginalis TVAG_441510   WD repeat containing protein, putative

Essentiality

Tb927.8.3860 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.3860 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.3860 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.3860 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.3860 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y18D10A.9 Caenorhabditis elegans embryonic lethal wormbase
YDR267C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0607800 Plasmodium berghei Essential plasmo
TGME49_313280 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.8.3860 (Trypanosoma brucei), cytosolic iron-sulfur protein assembly 1
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