Detailed view for Tb927.10.3150

Basic information

TDR Targets ID: 19331
Trypanosoma brucei, N-acetyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.3198 | Length (AA): 205 | MW (Da): 23621 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00583   Acetyltransferase (GNAT) family

Gene Ontology

Mouse over links to read term descriptions.
GO:0008080   N-acetyltransferase activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
25 191 2ob0 (C) 6 166 28.00 0 1 1.21833 -0.76
91 154 4m85 (A) 108 164 39.00 0.68 0.63 0.514995 0.84
111 163 2vi7 (A) 101 152 35.00 0.066 0.63 0.688337 -0.33

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128600)

Species Accession Gene Product
Arabidopsis thaliana AT5G11340   GCN5-related N-acetyltransferase (GNAT) family protein
Brugia malayi Bm1_06410   acetyltransferase, GNAT family protein
Candida albicans CaO19.490   potential N-acetyl tranferase (GNAT family) similar to S. cerevisiae NAT5 (YOR253W) peptide alpha-N-acetyltransferase
Candida albicans CaO19.8120   potential N-acetyl tranferase (GNAT family) similar to S. cerevisiae NAT5 (YOR253W) peptide alpha-N-acetyltransferase
Caenorhabditis elegans CELE_F40F4.7   Protein F40F4.7
Dictyostelium discoideum DDB_G0295721   GCN5-related N-acetyltransferase
Drosophila melanogaster Dmel_CG12352   separation anxiety
Echinococcus granulosus EgrG_000215400   n alpha acetyltransferase 50 NatE catalytic
Entamoeba histolytica EHI_139360   acetyltransferase, GNAT family
Echinococcus multilocularis EmuJ_000215400   n alpha acetyltransferase 50, NatE catalytic
Homo sapiens ENSG00000121579   N(alpha)-acetyltransferase 50, NatE catalytic subunit
Leishmania braziliensis LbrM.03.0140   acetyltransferase, putative
Leishmania donovani LdBPK_030120.1   acetyltransferase, putative
Leishmania infantum LinJ.03.0120   acetyltransferase, putative
Leishmania major LmjF.03.0130   acetyltransferase, putative
Leishmania mexicana LmxM.03.0130   acetyltransferase, putative
Loa Loa (eye worm) LOAG_11415   acetyltransferase
Mus musculus ENSMUSG00000022698   N(alpha)-acetyltransferase 50, NatE catalytic subunit
Neospora caninum NCLIV_026890   acetyltransferase domain-containing protein, putative
Oryza sativa 4327733   Os01g0610400
Saccharomyces cerevisiae YOR253W   Nat5p
Schistosoma japonicum Sjp_0120450   ko:K00680 Mak3 homolog [EC:2.3.1.-], putative
Schistosoma mansoni Smp_006780   hypothetical protein
Schmidtea mediterranea mk4.002874.01   N-alpha-acetyltransferase 50
Schmidtea mediterranea mk4.001198.01   N-alpha-acetyltransferase 50
Schmidtea mediterranea mk4.004031.01   N-alpha-acetyltransferase 50
Trypanosoma brucei gambiense Tbg972.10.3980   N-acetyltransferase, putative
Trypanosoma brucei Tb927.10.3150   N-acetyltransferase, putative
Trypanosoma congolense TcIL3000_10_2610   N-acetyltransferase, putative
Trypanosoma cruzi TcCLB.510943.100   acetyltransferase, putative
Trypanosoma cruzi TcCLB.504867.40   acetyltransferase, putative
Toxoplasma gondii TGME49_260010   acetyltransferase, GNAT family protein
Trichomonas vaginalis TVAG_363670   N-terminal acetyltransferase, putative
Trichomonas vaginalis TVAG_116150   N-acetyltransferase separation anxiety, putative

Essentiality

Tb927.10.3150 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.3150 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.3150 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.3150 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.3150 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_260010 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0004 0.5 0.5
0.0003 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.3150 (Trypanosoma brucei), N-acetyltransferase, putative
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