Detailed view for PF3D7_1146600

Basic information

TDR Targets ID: 1942
Plasmodium falciparum, oocyst rupture protein 1, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 4.3532 | Length (AA): 1301 | MW (Da): 150983 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00808   Histone-like transcription factor (CBF/NF-Y) and archaeal histone

Gene Ontology

Mouse over links to read term descriptions.
GO:0046982   protein heterodimerization activity  
GO:0006355   regulation of transcription, DNA-dependent  
GO:0005634   nucleus  
GO:0005622   intracellular  
GO:0043565   sequence-specific DNA binding  
GO:0003677   DNA binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 20 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1133 1218 1n1j (A) 56 141 59.00 0 1 0.85 -1.21
1135 1223 2byk (B) 11 99 31.00 0 1 0.56 -1.05
91 379 2aqw (A) 22 317 19.00 0.53 0.05 0.355337 -0.54
243 438 4v3d (A) 507 716 19.00 0.38 0.01 0.355853 -1
353 1043 3v0a (B) 373 1098 25.00 0.0000023 0.93 0.53763 0.94
961 1078 3ik2 (A) 388 493 32.00 0.55 0.22 0.322199 -0.52
962 1071 3ult (A) 6 113 32.00 0.0099 0.16 0.47805 -1.6
1127 1218 4g92 (B) 41 132 55.00 0.0000000025 1 0.816915 -1.32
1134 1218 4csr (A) 57 141 60.00 0 1 0.892834 -1.4
114 166 4m9v (C) 142 194 2.00 0.52 0 0.216949 -1.81
130 171 2emw (A) 1 37 11.00 0.71 0.01 0.151409 -1.63
250 451 4r58 (A) 20 218 24.00 0.25 0.94 0.508732 -0.9
406 706 3mpp (G) 890 1228 25.00 0.26 0.71 0.297596 0.85
948 1065 3ik2 (A) 388 493 32.00 0.56 0.22 0.323515 -0.52
949 1058 3ult (A) 6 113 32.00 0.01 0.16 0.479304 -1.6
970 1025 1x5w (A) 7 62 9.00 0.11 0 0.350278 -2.42
1114 1205 4g92 (B) 41 132 55.00 0.0000000012 1 0.817829 -1.32
1121 1205 4csr (A) 57 141 60.00 0 1 0.893894 -1.4
1130 1238 4wzs (B) 16 137 26.00 0.0046 0.53 0.400427 -0.28
1136 1247 1jfi (B) 127 235 25.00 0.000000099 0.61 0.415757 -0.63

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile merozoite, late schizont, Sporozoite. PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, sporozoite, early schizont, Oocyst. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 16 hs, gametocyte, late trophozoite, Ring, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 32 hs, early trophozoite. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 24 hs. Otto TD
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_127594)

Species Accession Gene Product
Arabidopsis thaliana AT4G14540   nuclear transcription factor Y subunit B-3
Arabidopsis thaliana AT2G38880   nuclear transcription factor Y subunit B-1
Arabidopsis thaliana AT5G47640   nuclear transcription factor Y subunit B-2
Arabidopsis thaliana AT3G53340   nuclear transcription factor Y subunit B-10
Arabidopsis thaliana AT2G37060   nuclear transcription factor Y subunit B-8
Arabidopsis thaliana AT2G13570   nuclear transcription factor Y subunit B-7
Babesia bovis BBOV_I000180   histone-like transcription factor domain containing protein
Brugia malayi Bm1_08875   hypothetical protein
Candida albicans CaO19.8148   one of two genes similar to S. cerevisiae HAP3 (YBL021C) histone-like transcription factor, CCAAT Binding Factor (CBF)
Candida albicans CaO19.4647   one of two genes similar to S. cerevisiae HAP3 (YBL021C) histone-like transcription factor, CCAAT Binding Factor (CBF)
Candida albicans CaO19.517   one of two genes similar to S. cerevisiae HAP3 (YBL021C) histone-like transcription factor, CCAAT Binding Factor (CBF)
Candida albicans CaO19.12116   one of two genes similar to S. cerevisiae HAP3 (YBL021C) histone-like transcription factor, CCAAT Binding Factor (CBF)
Caenorhabditis elegans CELE_W10D9.4   Protein NFYB-1
Cryptosporidium hominis Chro.40195   CCAAT-box DNA binding protein subunit B
Cryptosporidium parvum cgd4_1720   CCAAT-box DNA binding protein subunit B
Dictyostelium discoideum DDB_G0279419   hypothetical protein
Drosophila melanogaster Dmel_CG10447   Nuclear factor Y-box B
Echinococcus granulosus EgrG_000204400   Nuclear transcription factor Y subunit beta
Entamoeba histolytica EHI_168220   CBF/NF-Y transcription factor family protein
Echinococcus multilocularis EmuJ_000204400   Nuclear transcription factor Y subunit beta
Giardia lamblia GL50803_7231   CCAAT-binding transcription factor subunit A
Homo sapiens ENSG00000120837   nuclear transcription factor Y, beta
Loa Loa (eye worm) LOAG_12420   nuclear transcription factor Y subunit beta
Mus musculus ENSMUSG00000020248   nuclear transcription factor-Y beta
Oryza sativa 4333105   Os03g0413000
Oryza sativa 4343856   Os07g0606600
Oryza sativa 4339676   Os05g0573500
Plasmodium berghei PBANKA_0902500   oocyst rupture protein 1
Plasmodium falciparum PF3D7_1146600   oocyst rupture protein 1, putative
Plasmodium knowlesi PKNH_0944400   oocyst rupture protein 1, putative
Plasmodium vivax PVX_092925   CCAAT-box DNA binding protein subunit B, putative
Plasmodium yoelii PY05259   CCAAT-box DNA binding protein subunit B
Saccharomyces cerevisiae YBL021C   Hap3p
Schistosoma japonicum Sjp_0310520   ko:K08065 nuclear transcription factor Y, beta, putative
Schistosoma mansoni Smp_154340   nuclear factor Y transcription factor subunit B homolog
Schmidtea mediterranea mk4.002264.14   Nuclear factor Y subunit B
Theileria parva TP03_0845   hypothetical protein

Essentiality

PF3D7_1146600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0902500 Plasmodium berghei Slow plasmo
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1146600 (Plasmodium falciparum), oocyst rupture protein 1, putative
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