Detailed view for Tb927.10.1910

Basic information

TDR Targets ID: 19596
Trypanosoma brucei, STE/STE11 serine/threonine-protein kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.1665 | Length (AA): 1534 | MW (Da): 168191 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 1

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00211   Adenylate and Guanylate cyclase catalytic domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0035556   GO:intracellular signal transduction  

GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0016849   phosphorus-oxygen lyase activity  
GO:0005524   ATP binding  
GO:0009190   cyclic nucleotide biosynthetic process  
GO:0007242   intracellular signaling cascade  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_145063)

Species Accession Gene Product
Leishmania braziliensis LbrM.21.0180   protein kinase, putative,serine/threonine protein kinase, putative
Leishmania braziliensis LbrM.21.0190   protein kinase, putative,serine/threonine protein kinase, putative
Leishmania donovani LdBPK_210190.1   serine/threonine protein kinase, putative
Leishmania infantum LinJ.21.0190   protein kinase, putative,serine/threonine protein kinase, putative
Leishmania major LmjF.21.0130   protein kinase, putative,serine/threonine protein kinase, putative
Leishmania mexicana LmxM.21.0130   protein kinase, putative,serine/threonine protein kinase, putative
Trypanosoma brucei gambiense Tbg972.10.2310   protein kinase, putative,serine/threonine protein kinase, putative
Trypanosoma brucei Tb11.v5.0556   protein kinase, putative
Trypanosoma brucei Tb927.10.1910   STE/STE11 serine/threonine-protein kinase, putative
Trypanosoma congolense TcIL3000_10_1620   serine/threonine protein kinase, putative
Trypanosoma cruzi TcCLB.506493.70   STE/STE11 serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.510121.150   STE/STE11 serine/threonine-protein kinase, putative

Essentiality

Tb927.10.1910 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.1910 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.1910 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.1910 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.10.1910 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 27.8% 284 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 25.6% 266 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 26.4% 296 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 25.3% 289 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 24.8% 286 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 27.3% 209 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0007 0.5 0.5
0.0016 0.5 0.5
0.0033 1 0.5
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0037 1 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0032 0.5 0.5
0.0093 0.8828 0
0.0056 1 0.5
0.0088 0.4477 0.5
0.0081 1 0.5
0.0064 0.3377 0
0.0022 0.5 0.5
0.0059 1 1
0.0063 0.7244 0.2543
0.0059 1 1
0.0062 0.6935 0
0.0029 0.5 0.5
0.0011 1 0.5
0.0092 1 0.5
0.0008 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0036 0.5 0.5
0.0039 0.5 0.5
0.0061 0.6883 0.5304
0.0026 0.5 0.5
0.0066 0.3101 0
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0027 1 0.5
0.0059 1 1
0.0069 0.3067 1
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0007 0.5 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0063 1 0.5
0.0033 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.1910 (Trypanosoma brucei), STE/STE11 serine/threonine-protein kinase, putative
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