Detailed view for Tb11.v5.0258

Basic information

TDR Targets ID: 19604
Trypanosoma brucei, Sperm tail/Sperm tail C-terminal domain containing protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.5793 | Length (AA): 507 | MW (Da): 58672 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF14772   Sperm tail

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
15 357 5h7c (A) 49 401 24.00 0.19 1 0.802329 0.09
78 359 4tql (A) 10 232 29.00 0.00011 1 0.600013 0.31
148 333 4uos (A) 1 185 13.00 0 0.06 0.601964 -1.71
204 385 4uos (A) 9 184 25.00 0.64 1 0.746774 -1.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129935)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG30259   CG30259 gene product from transcript CG30259-RA
Echinococcus granulosus EgrG_001052300   coiled coil domain containing protein 65
Echinococcus multilocularis EmuJ_001052300   coiled coil domain containing protein 65
Giardia lamblia GL50803_23357   NYD-SP28 protein
Homo sapiens ENSG00000139537   coiled-coil domain containing 65
Leishmania braziliensis LbrM.28.0060   hypothetical protein, conserved
Leishmania donovani LdBPK_280050.1   nexin-dynein regulatory complex 2
Leishmania infantum LinJ.28.0050   hypothetical protein, conserved
Leishmania major LmjF.28.0050   hypothetical protein, conserved
Leishmania mexicana LmxM.28.0050   hypothetical protein, conserved
Mus musculus ENSMUSG00000003354   coiled-coil domain containing 65
Neospora caninum NCLIV_015070   hypothetical protein
Plasmodium berghei PBANKA_0721100   conserved Plasmodium protein, unknown function
Plasmodium falciparum PF3D7_0419000   conserved Plasmodium protein, unknown function
Plasmodium knowlesi PKNH_0511700   conserved Plasmodium protein, unknown function
Plasmodium vivax PVX_090040   hypothetical protein, conserved
Schistosoma japonicum Sjp_0301480   Conserved hypothetical protein
Schistosoma mansoni Smp_087560   hypothetical protein
Schmidtea mediterranea mk4.007636.00  
Schmidtea mediterranea mk4.007341.02  
Trypanosoma brucei gambiense Tbg972.11.8260   hypothetical protein, conserved
Trypanosoma brucei Tb11.v5.0258   Sperm tail/Sperm tail C-terminal domain containing protein, putative
Trypanosoma congolense TcIL3000_0_45610   nexin-dynein regulatory complex 2
Trypanosoma cruzi TcCLB.508793.14   nexin-dynein regulatory complex 2
Trypanosoma cruzi TcCLB.506779.10   component of motile flagella 70 protein
Trypanosoma cruzi TcCLB.508239.49   component of motile flagella 70 protein
Toxoplasma gondii TGME49_284670   flagellar associated protein
Trichomonas vaginalis TVAG_330860   DNA double-strand break repair Rad50 ATPase, putative
Trichomonas vaginalis TVAG_094580   conserved hypothetical protein

Essentiality

Tb11.v5.0258 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.5150 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.5150 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.5150 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.5150 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_284670 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb11.v5.0258 (Trypanosoma brucei), Sperm tail/Sperm tail C-terminal domain containing protein, putative
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