Detailed view for Tb927.10.13517

Basic information

TDR Targets ID: 19669
Trypanosoma brucei, 40S ribosomal protein S27-like, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.9396 | Length (AA): 86 | MW (Da): 9630 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01667   Ribosomal protein S27

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 84 4kzy (b) 3 83 60.00 0 1 1.59626 0.4
30 78 1qxf (A) 2 50 37.00 0 0.96 1.02807 -0.09

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3ZEY:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127008)

Species Accession Gene Product
Arabidopsis thaliana AT5G47930   40S ribosomal protein S27-3
Arabidopsis thaliana AT3G61111   zinc-binding ribosomal protein family protein
Arabidopsis thaliana AT2G45710   40S ribosomal protein S27-1
Arabidopsis thaliana AT3G61110   40S ribosomal protein S27-2
Babesia bovis BBOV_III003560   40S ribosomal protein S27, putative
Brugia malayi Bm1_03910   40S ribosomal protein S27
Caenorhabditis elegans CELE_F56E10.4   Protein RPS-27
Cryptosporidium hominis Chro.70173   40S ribosomal protein S27
Cryptosporidium parvum cgd7_1460   ribosomal protein S27
Dictyostelium discoideum DDB_G0277635   40S ribosomal protein S27
Drosophila melanogaster Dmel_CG10423   Ribosomal protein S27
Echinococcus granulosus EgrG_000297000   ribosomal protein S27
Entamoeba histolytica EHI_152230   40S ribosomal protein S27, putative
Entamoeba histolytica EHI_004200   40S ribosomal protein S27, putative
Entamoeba histolytica EHI_149000   40S ribosomal protein S27
Echinococcus multilocularis EmuJ_000297000   ribosomal protein S27
Giardia lamblia GL50803_10780   Ribosomal protein S27
Homo sapiens ENSG00000177954   ribosomal protein S27
Homo sapiens 51065   ribosomal protein S27-like
Leishmania braziliensis LbrM.35.3990   40S ribosomal protein S27-1, putative
Leishmania braziliensis LbrM.18.0670   40S ribosomal protein S27-1
Leishmania donovani LdBPK_363940.1   40S ribosomal protein S27-1, putative
Leishmania infantum LinJ.36.3940   40S ribosomal protein S27-1, putative
Leishmania major LmjF.36.3750   40S ribosomal protein S27-1, putative
Leishmania mexicana LmxM.36.3750   40S ribosomal protein S27-1, putative
Loa Loa (eye worm) LOAG_03894   40S ribosomal protein S27
Mus musculus 57294   ribosomal protein S27
Mus musculus ENSMUSG00000050621   ribosomal protein S27, retrogene
Mus musculus ENSMUSG00000036781   ribosomal protein S27-like
Neospora caninum NCLIV_062350   Ribosomal protein S27, related
Oryza sativa 4329362   Os02g0478700
Oryza sativa 4335531   Os04g0346100
Plasmodium berghei PBANKA_1406800   40S ribosomal protein S27, putative
Plasmodium falciparum PF3D7_1308300   40S ribosomal protein S27
Plasmodium knowlesi PKNH_1408700   40S ribosomal protein S27, putative
Plasmodium vivax PVX_122245   40S ribosomal protein S27, putative
Plasmodium yoelii PY03153   ribosomal protein S27
Saccharomyces cerevisiae YKL156W   ribosomal 40S subunit protein S27A
Saccharomyces cerevisiae YHR021C   ribosomal 40S subunit protein S27B
Schistosoma japonicum Sjp_0202200   ko:K02978 small subunit ribosomal protein S27e, putative
Schistosoma mansoni Smp_041650   40S ribosomal protein S27
Schmidtea mediterranea mk4.008691.00   40S ribosomal protein S27
Trypanosoma brucei gambiense Tbg972.11.10910   40S ribosomal protein S27, putative
Trypanosoma brucei Tb927.10.13517   40S ribosomal protein S27-like, putative
Trypanosoma cruzi TcCLB.506963.14   40S ribosomal protein S27, putative
Trypanosoma cruzi TcCLB.511233.14   40S ribosomal protein S27, putative
Trypanosoma cruzi TcCLB.511277.175   ribosomal protein S27, putative
Toxoplasma gondii TGME49_217570   ribosomal protein RPS27
Theileria parva TP03_0052   40S ribosomal protein S27, putative
Trichomonas vaginalis TVAG_064320   40S ribosomal protein S27, putative
Trichomonas vaginalis TVAG_378970   40S ribosomal protein S27, putative
Trichomonas vaginalis TVAG_110520   40S ribosomal protein S27, putative
Trichomonas vaginalis TVAG_356540   40S ribosomal protein S27, putative

Essentiality

Tb927.10.13517 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1475 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.1475 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.1475 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.01.1475 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F56E10.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F56E10.4 Caenorhabditis elegans larval arrest wormbase
CELE_F56E10.4 Caenorhabditis elegans slow growth wormbase
PBANKA_1406800 Plasmodium berghei Essential plasmo
TGME49_217570 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens ribosomal protein S27 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.10.13517 (Trypanosoma brucei), 40S ribosomal protein S27-like, putative
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