Detailed view for PF3D7_1149600

Basic information

TDR Targets ID: 1978
Plasmodium falciparum, DnaJ protein, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.0043 | Length (AA): 714 | MW (Da): 83120 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00226   DnaJ domain
PF14308   X-domain of DnaJ-containing

Gene Ontology

Mouse over links to read term descriptions.
GO:0051082   unfolded protein binding  
GO:0031072   heat shock protein binding  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
175 267 2ctw (A) 6 98 37.00 0 1 0.63 -1.04
187 252 2ctp (A) 8 72 46.00 0.0000000034 1 0.86 -2.26
302 386 2yua (A) 6 89 38.00 0.000000018 1 0.702648 -1.2
312 381 2lo1 (A) 5 71 55.00 0 1 0.886639 -1.85
314 379 2ctr (A) 8 72 48.00 0.00000000074 1 0.904037 -2.48
315 379 2dn9 (A) 9 73 54.00 0.000000000039 1 0.990636 -2.66
304 388 2yua (A) 6 89 38.00 0.000000014 1 0.700015 -1.2
314 383 2lo1 (A) 5 71 55.00 0 1 0.884065 -1.85
316 381 2ctr (A) 8 72 48.00 0.00000000055 1 0.901479 -2.48
317 381 2dn9 (A) 9 73 54.00 0.000000000028 1 0.988082 -2.66

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, merozoite, sporozoite, early ring, late ring, late schizont, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile early trophozoite, late trophozoite. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 24 hs, early schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Sporozoite. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Oocyst, Female Gametocyte, Male Gametocyte. Zanghi G Lasonder E
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_129626)

Species Accession Gene Product
Arabidopsis thaliana AT1G77020   DNAJ heat shock N-terminal domain-containing protein
Arabidopsis thaliana AT1G76700   chaperone protein dnaJ 10
Arabidopsis thaliana AT2G21510   DNAJ heat shock N-terminal domain-containing protein
Arabidopsis thaliana AT1G21080   DNAJ heat shock N-terminal domain-containing protein
Arabidopsis thaliana AT4G39150   DNAJ heat shock N-terminal domain-containing protein
Babesia bovis BBOV_III003820   dnaJ domain containing protein
Cryptosporidium hominis Chro.80380   hypothetical protein
Cryptosporidium parvum cgd8_3270   DNAJDNAJ protein
Dictyostelium discoideum DDB_G0267994   DNAJ heat shock N-terminal domain-containing protein
Leishmania braziliensis LbrM.31.0670   hypothetical protein, conserved
Leishmania donovani LdBPK_310530.1   DnaJ domain containing protein, putative
Leishmania infantum LinJ.31.0530   hypothetical protein, conserved
Leishmania major LmjF.31.0510   hypothetical protein, conserved
Leishmania mexicana LmxM.30.0510   hypothetical protein, conserved
Neospora caninum NCLIV_063240   DnaJ homologue, related
Oryza sativa 4329657   Os02g0555700
Oryza sativa 4339491   Os05g0543700
Oryza sativa 4346080   Os08g0522600
Oryza sativa 4347450   Os09g0493800
Oryza sativa 9268042   Os01g0702450
Plasmodium berghei PBANKA_1238200   DnaJ protein, putative
Plasmodium falciparum PF3D7_0523400   DnaJ protein, putative
Plasmodium falciparum PF3D7_1149600   DnaJ protein, putative
Plasmodium knowlesi PKNH_1009500   DnaJ protein, putative
Plasmodium vivax PVX_080080   hypothetical protein, conserved
Plasmodium yoelii PY03711   Arabidopsis thaliana At4g39150/T22F8_50, putative
Saccharomyces cerevisiae YER048C   Caj1p
Trypanosoma brucei gambiense Tbg972.8.8650   chaperone protein DNAj, putative
Trypanosoma brucei Tb927.8.8310   chaperone protein DnaJ, putative
Trypanosoma congolense TcIL3000_10_13980   chaperone protein DnaJ, putative
Trypanosoma cruzi TcCLB.506387.9   chaperone DNAJ protein, putative
Trypanosoma cruzi TcCLB.511337.10   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508555.10   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.509237.80   hypothetical protein, conserved
Toxoplasma gondii TGME49_246340   DnaJ domain-containing protein
Theileria parva TP02_0221   hypothetical protein, conserved

Essentiality

PF3D7_1149600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.8310 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.8310 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.8310 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.8310 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1238200 Plasmodium berghei Slow plasmo
TGME49_246340 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_1149600 (Plasmodium falciparum), DnaJ protein, putative
Title for this comment
Comment