Detailed view for Tb927.7.6350

Basic information

TDR Targets ID: 19784
Trypanosoma brucei, NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.6904 | Length (AA): 273 | MW (Da): 30925 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01257   Thioredoxin-like [2Fe-2S] ferredoxin

Gene Ontology

Mouse over links to read term descriptions.
GO:0051287   NAD binding  
GO:0016491   oxidoreductase activity  
GO:0009055   electron carrier activity  
GO:0055114   oxidation reduction  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
57 224 3i9v (2) 7 165 33.00 0 1 1.04558 -0.59
129 221 1m2d (A) 4 95 21.00 0 1 0.506459 -0.99

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127830)

Species Accession Gene Product
Arabidopsis thaliana AT4G02580   NADH dehydrogenase (ubiquinone) flavoprotein 2
Brugia malayi Bm1_20470   NADH-ubiquinone oxidoreductase 24 kDa subunit, mitochondrial precursor
Candida albicans CaO19.2091   potential mitochondrial NADH-Ubiquinone Oxidoreductase Complex I subunit similar to Y.lipolytica NUHM, N. crassa and mammalian 2
Candida albicans CaO19.9638   potential mitochondrial NADH-Ubiquinone Oxidoreductase Complex I subunit similar to Y.lipolytica NUHM, N. crassa and mammalian 2
Caenorhabditis elegans CELE_F53F4.10   Protein F53F4.10
Dictyostelium discoideum DDB_G0291173   NADH dehydrogenase
Drosophila melanogaster Dmel_CG5703   CG5703 gene product from transcript CG5703-RA
Escherichia coli b2285   NADH:ubiquinone oxidoreductase, chain E
Echinococcus granulosus EgrG_001114700   NADH dehydrogenase ubiquinone flavoprotein 2
Echinococcus multilocularis EmuJ_001114700   NADH dehydrogenase (ubiquinone) flavoprotein 2
Homo sapiens ENSG00000178127   NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
Leishmania braziliensis LbrM.17.0250   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania donovani LdBPK_170320.1   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania infantum LinJ.17.0320   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania major LmjF.17.0270   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania mexicana LmxM.17.0270   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Loa Loa (eye worm) LOAG_05848   NADH-ubiquinone oxidoreductase 24 kDa subunit
Mus musculus ENSMUSG00000024099   NADH dehydrogenase (ubiquinone) flavoprotein 2
Mycobacterium tuberculosis Rv3149   Probable NADH dehydrogenase I (chain E) NuoE (NADH-ubiquinone oxidoreductase chain E)
Mycobacterium ulcerans MUL_2463   NADH dehydrogenase subunit E
Oryza sativa 4339271   Os05g0509200
Onchocerca volvulus OVOC1929   Putative NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
Schistosoma japonicum Sjp_0304770   ko:K03943 NADH dehydrogenase (ubiquinone) flavoprotein 2, putative
Schistosoma mansoni Smp_069770   NADH-ubiquinone oxidoreductase
Schmidtea mediterranea mk4.070622.01  
Schmidtea mediterranea mk4.004744.01   Probable NADH dehydrogenase
Trypanosoma brucei gambiense Tbg972.7.7330   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trypanosoma brucei Tb927.7.6350   NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
Trypanosoma congolense TcIL3000_7_5250   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trypanosoma cruzi TcCLB.511323.50   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trypanosoma cruzi TcCLB.506513.190   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trichomonas vaginalis TVAG_296220   NADH dehydrogenase 24 kDa subunit, putative
Wolbachia endosymbiont of Brugia malayi Wbm0594   NADH dehydrogenase subunit E

Essentiality

Tb927.7.6350 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.6350 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.6350 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.6350 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.6350 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F53F4.10 Caenorhabditis elegans embryonic lethal wormbase
CELE_F53F4.10 Caenorhabditis elegans larval arrest wormbase
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.7.6350 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 2 )

Bibliographic References

9 literature references were collected for this gene.

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Gene identifier Tb927.7.6350 (Trypanosoma brucei), NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
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