Detailed view for PF3D7_1357900

Basic information

TDR Targets ID: 199
Plasmodium falciparum, pyrroline-5-carboxylate reductase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.0666 | Length (AA): 262 | MW (Da): 28379 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03807   NADP oxidoreductase coenzyme F420-dependent
PF14748   Pyrroline-5-carboxylate reductase dimerisation

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0005488   binding  
GO:0004735   pyrroline-5-carboxylate reductase activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  
GO:0006561   proline biosynthetic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 240 1f0y (A) 13 288 10.00 0 0.88 0.81 -0.64
1 262 1np3 (A) 14 307 13.00 0 1 0.92 0.06
4 259 2ahr (A) 1 256 32.00 0 1 1.41 -0.59
1 261 2rcy (A) 2 262 99.99 0 1 2.20268 -1.15

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2RCY:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, gametocyte, sporozoite, early ring, early schizont, early trophozoite, late ring, late trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, merozoite, late schizont, Oocyst, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, Sporozoite. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Male Gametocyte. Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_126801)

Species Accession Gene Product
Arabidopsis thaliana AT5G14800   Pyrroline-5-carboxylate reductase
Brugia malayi Bm1_38015   pyrroline-5-carboxylate reductase family protein
Candida albicans CaO19.5650   Delta-1-pyrroline-5-carboxylate reductase
Candida albicans CaO19.13095   Delta-1-pyrroline-5-carboxylate reductase
Caenorhabditis elegans CELE_M153.1   Protein M153.1
Cryptosporidium hominis Chro.60426   pyrroline-5-carboxylate reductase
Cryptosporidium parvum cgd6_3720   pyrroline-5-carboxylate reductase
Dictyostelium discoideum DDB_G0269410   PCA reductase
Dictyostelium discoideum DDB_G0288671   PCA reductase
Drosophila melanogaster Dmel_CG6009   Pyrroline 5-carboyxlate reductase
Drosophila melanogaster Dmel_CG5840   Pyrroline-5-carboxylate reductase-like 2
Escherichia coli b0386   pyrroline-5-carboxylate reductase, NAD(P)-binding
Echinococcus granulosus EgrG_000233100   pyrroline 5 carboxylate reductase
Echinococcus multilocularis EmuJ_000233100   pyrroline 5 carboxylate reductase
Homo sapiens ENSG00000104524   pyrroline-5-carboxylate reductase-like
Homo sapiens ENSG00000183010   pyrroline-5-carboxylate reductase 1
Leishmania braziliensis LbrM.13.1540   pyrroline-5-carboxylate reductase
Leishmania donovani LdBPK_131420.1   pyrroline-5-carboxylate reductase
Leishmania infantum LinJ.13.1420   pyrroline-5-carboxylate reductase
Leishmania major LmjF.13.1680   pyrroline-5-carboxylate reductase
Leishmania mexicana LmxM.13.1680   pyrroline-5-carboxylate reductase
Loa Loa (eye worm) LOAG_07911   hypothetical protein
Mycobacterium leprae ML2430c   PROBABLE PYRROLINE-5-CARBOXYLATE REDUCTASE PROC (P5CR) (P5C REDUCTASE)
Mus musculus ENSMUSG00000022571   pyrroline-5-carboxylate reductase-like
Mus musculus ENSMUSG00000025140   pyrroline-5-carboxylate reductase 1
Mus musculus ENSMUSG00000026520   pyrroline-5-carboxylate reductase family, member 2
Mycobacterium tuberculosis Rv0500   Probable pyrroline-5-carboxylate reductase ProC (P5CR) (P5C reductase)
Mycobacterium ulcerans MUL_4570   pyrroline-5-carboxylate reductase
Neospora caninum NCLIV_050390   Pyrroline-5-carboxylate reductase (EC 1.5.1.2), related
Oryza sativa 4325755   Os01g0948400
Plasmodium berghei PBANKA_1134200   pyrroline-5-carboxylate reductase, putative
Plasmodium falciparum PF3D7_1357900   pyrroline-5-carboxylate reductase
Plasmodium knowlesi PKNH_1113600   pyrroline-5-carboxylate reductase, putative
Plasmodium vivax PVX_114870   pyrroline carboxylate reductase, putative
Plasmodium yoelii PY02938   pyrroline-5-carboxylate reductase
Saccharomyces cerevisiae YER023W   pyrroline-5-carboxylate reductase
Schistosoma japonicum Sjp_0303670   ko:K00286 pyrroline-5-carboxylate reductase [EC1.5.1.2], putative
Schistosoma mansoni Smp_049390   pyrroline-5-carboxylate reductase
Schmidtea mediterranea mk4.015283.01  
Schmidtea mediterranea mk4.023354.00   Pyrroline-5-carboxylate reductase
Trypanosoma brucei gambiense Tbg972.7.2720   pyrroline-5-carboxylate reductase, putative
Trypanosoma brucei Tb927.7.2440   pyrroline-5-carboxylate reductase, putative
Trypanosoma congolense TcIL3000_7_1750   pyrroline-5-carboxylate reductase, putative
Trypanosoma cruzi TcCLB.509207.90   pyrroline-5-carboxylate reductase, putative
Trypanosoma cruzi TcCLB.506857.20   pyrroline-5-carboxylate reductase, putative
Toxoplasma gondii TGME49_236070   pyrroline-5-carboxylate reductase
Treponema pallidum TP0797   1-pyrroline-5-carboxylate reductase (proC)
Trichomonas vaginalis TVAG_423640   pyrroline-5-carboxylate reductase, putative
Trichomonas vaginalis TVAG_063510   pyrroline-5-carboxylate reductase, putative

Essentiality

PF3D7_1357900 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu506 Mycobacterium tuberculosis essential nmpdr
Tb927.7.2440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.2440 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.2440 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.2440 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0386 Escherichia coli non-essential goodall
YER023W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1134200 Plasmodium berghei Dispensable plasmo
TGME49_236070 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Arabidopsis thaliana Pyrroline-5-carboxylate reductase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_1357900 (Plasmodium falciparum), pyrroline-5-carboxylate reductase
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