Detailed view for Tb927.4.2740

Basic information

TDR Targets ID: 20027
Trypanosoma brucei, p25-alpha, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.186 | Length (AA): 150 | MW (Da): 16328 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05517   p25-alpha

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 150 2jrf (A) 10 165 35.00 0.000000002 1 1.306 0.05
2 63 1c7v (A) 88 147 20.00 0.14 0.04 0.549533 0.29
2 79 5d43 (A) 31 105 15.00 0.041 0.13 0.6912 -0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129676)

Species Accession Gene Product
Babesia bovis BBOV_IV008480   conserved hypothetical protein
Caenorhabditis elegans CELE_C32E8.3   Protein C32E8.3
Drosophila melanogaster Dmel_CG45057   CG45057 gene product from transcript CG45057-RA
Echinococcus granulosus EgrG_001093300   tubulin polymerization promoting protein family
Echinococcus granulosus EgrG_001093400   tubulin polymerization promoting protein family
Echinococcus granulosus EgrG_000333800   tubulin polymerization promoting protein family
Echinococcus granulosus EgrG_000096900   tubulin polymerization promoting protein family
Echinococcus multilocularis EmuJ_001093300   tubulin polymerization promoting protein family
Echinococcus multilocularis EmuJ_000096900   tubulin polymerization promoting protein family
Echinococcus multilocularis EmuJ_001093400   tubulin polymerization promoting protein family
Echinococcus multilocularis EmuJ_000333800   tubulin polymerization promoting protein family
Homo sapiens ENSG00000179636   tubulin polymerization-promoting protein family member 2
Homo sapiens ENSG00000159713   tubulin polymerization-promoting protein family member 3
Leishmania braziliensis LbrM.14.1190   hypothetical protein, conserved
Leishmania braziliensis LbrM.14.1180   hypothetical protein, conserved
Leishmania donovani LdBPK_341630.1   p25-alpha, putative
Leishmania infantum LinJ.34.1620   hypothetical protein, conserved
Leishmania infantum LinJ.34.1630   hypothetical protein, conserved
Leishmania major LmjF.34.1530   hypothetical protein, conserved
Leishmania major LmjF.34.1520   hypothetical protein, conserved
Leishmania mexicana LmxM.33.1520   hypothetical protein, conserved
Mus musculus ENSMUSG00000014846   tubulin polymerization-promoting protein family member 3
Mus musculus ENSMUSG00000008813   tubulin polymerization-promoting protein family member 2
Neospora caninum NCLIV_036160   hypothetical protein
Neospora caninum NCLIV_030630   p25-alpha family protein, related
Neospora caninum NCLIV_009710   Tubulin polymerization-promoting protein family member 3, related
Plasmodium berghei PBANKA_1451200   p25-alpha family protein, putative
Plasmodium falciparum PF3D7_1236600   p25-alpha family protein, putative
Plasmodium knowlesi PKNH_1456300   p25-alpha family protein, putative
Plasmodium vivax PVX_100780   p25-alpha family protein, putative
Plasmodium yoelii PY05543   Tetrahymena thermophila Igr3p
Schistosoma japonicum Sjp_0070200   Tubulin polymerization-promoting protein, putative
Schistosoma mansoni Smp_097490   P25 alpha-related
Schmidtea mediterranea mk4.005654.03   TPPP family protein
Schmidtea mediterranea mk4.001175.11   TPPP family protein
Schmidtea mediterranea mk4.001254.03   TPPP family protein
Trypanosoma brucei gambiense Tbg972.4.2720   hypothetical protein, conserved
Trypanosoma brucei Tb927.4.2740   p25-alpha, putative
Trypanosoma congolense TcIL3000_4_2660   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506635.130   p25-alpha, putative
Trypanosoma cruzi TcCLB.504199.20   p25-alpha, putative
Toxoplasma gondii TGME49_320560   p25-alpha family protein
Toxoplasma gondii TGME49_229930   p25-alpha family protein
Theileria parva TP01_0928   hypothetical protein

Essentiality

Tb927.4.2740 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2740 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.2740 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.2740 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.2740 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C32E8.3 Caenorhabditis elegans larval arrest wormbase
PBANKA_1451200 Plasmodium berghei Fast plasmo
TGME49_229930 Toxoplasma gondii Probably non-essential sidik
TGME49_320560 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tbru015101AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru015101; Recombinant protein: full-length; Source: T brucei; hypothetical protein, conserved ;

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.4.2740 (Trypanosoma brucei), p25-alpha, putative
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