Detailed view for Tb927.11.7410

Basic information

TDR Targets ID: 20056
Trypanosoma brucei, MSP-A, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4052 | Length (AA): 675 | MW (Da): 73405 | Paralog Number: 14

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01457   Leishmanolysin

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0008270   zinc ion binding  
GO:0004222   metalloendopeptidase activity  
GO:0007155   cell adhesion  
GO:0006508   proteolysis  

Metabolic Pathways

African trypanosomiasis (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
76 539 1lml (A) 103 566 25.00 0 1 0.923507 0.22
97 545 1lml (A) 124 572 32.00 0 1 0.965585 0.13
581 642 4nn5 (A) 52 128 37.00 0.05 0.29 0.241552 -0.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126749)

Species Accession Gene Product
Arabidopsis thaliana AT5G42620   metalloendopeptidase / zinc ion binding protein
Brugia malayi Bm1_53560   RH66426p
Caenorhabditis elegans CELE_Y43F4A.1   Protein Y43F4A.1, isoform B
Dictyostelium discoideum DDB_G0286533   hypothetical protein
Dictyostelium discoideum DDB_G0282305   hypothetical protein
Dictyostelium discoideum DDB_G0268270   hypothetical protein
Dictyostelium discoideum DDB_G0293364   peptidase M8, leishmanolysin family protein
Drosophila melanogaster Dmel_CG3953   CG3953 gene product from transcript CG3953-RA
Echinococcus granulosus EgrG_000839500   invadolysin M08 family
Entamoeba histolytica EHI_042870   cell surface protease gp63, putative
Entamoeba histolytica EHI_200230   cell surface protease gp63, putative
Echinococcus multilocularis EmuJ_000839500   invadolysin (M08 family)
Homo sapiens ENSG00000185621   leishmanolysin-like (metallopeptidase M8 family)
Leishmania braziliensis LbrM.10.0550   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania braziliensis LbrM.10.1680   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.1630   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.0540   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania braziliensis LbrM.10.0500   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania braziliensis LbrM.10.0520   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania braziliensis LbrM.10.0610   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.0510   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania braziliensis LbrM.10.1590   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.0470   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.0560   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania braziliensis LbrM.10.1650   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.1660   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.0590   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.1550   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.1610   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.0580   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania braziliensis LbrM.10.1620   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.1640   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.0480   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania braziliensis LbrM.10.1570   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.1690   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.0570   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania braziliensis LbrM.10.0530   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania braziliensis LbrM.10.1670   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.1560   GP63, leishmanolysin
Leishmania braziliensis LbrM.10.0600   GP63, leishmanolysin
Leishmania donovani LdBPK_280600.1   major surface protease gp63, putative
Leishmania donovani LdBPK_100520.1   GP63, leishmanolysin
Leishmania infantum LinJ.10.0500   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania infantum LinJ.10.0530   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania infantum LinJ.28.0600   major surface protease gp63, putative,leishmanolysin, putative
Leishmania infantum LinJ.10.0490   GP63, leishmanolysin
Leishmania infantum LinJ.10.0520   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania infantum LinJ.28.0610   major surface protease gp63, putative,leishmanolysin, putative
Leishmania infantum LinJ.10.0510   GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8
Leishmania major LmjF.10.0465   GP63, leishmanolysin
Leishmania major LmjF.10.0460   GP63, leishmanolysin
Leishmania major LmjF.10.0470   GP63, leishmanolysin
Leishmania major LmjF.28.0570   major surface protease gp63, putative,leishmanolysin, putative
Leishmania major LmjF.10.0480   GP63, leishmanolysin
Leishmania mexicana LmxM.10.0465   GP63, leishmanolysin
Leishmania mexicana LmxM.28.0570   major surface protease gp63, putative,leishmanolysin, putative
Leishmania mexicana LmxM.10.0405   GP63, leishmanolysin
Leishmania mexicana LmxM.10.0470   GP63, leishmanolysin
Leishmania mexicana LmxM.10.0465a  
Leishmania mexicana LmxM.10.0460   GP63, leishmanolysin
Leishmania mexicana LmxM.10.0390   GP63, leishmanolysin
Loa Loa (eye worm) LOAG_08471   hypothetical protein
Mus musculus 239833   leishmanolysin-like (metallopeptidase M8 family)
Oryza sativa 4334001   Os03g0731500
Schistosoma japonicum Sjp_0108000   ko:K01404 leishmanolysin [EC3.4.24.36], putative
Schistosoma japonicum Sjp_0067490   Leishmanolysin-like peptidase, putative
Schistosoma japonicum Sjp_0048370   Leishmanolysin-like peptidase, putative
Schistosoma mansoni Smp_127030   invadolysin (M08 family)
Schistosoma mansoni Smp_090110   invadolysin (M08 family)
Schistosoma mansoni Smp_153930   invadolysin (M08 family)
Schistosoma mansoni Smp_090100   invadolysin (M08 family)
Schmidtea mediterranea mk4.019252.00  
Schmidtea mediterranea mk4.028879.00  
Schmidtea mediterranea mk4.005302.00  
Schmidtea mediterranea mk4.032713.00  
Schmidtea mediterranea mk4.009763.00  
Schmidtea mediterranea mk4.002584.02   Leishmanolysin-like peptidase
Schmidtea mediterranea mk4.019481.00  
Schmidtea mediterranea mk4.003972.02  
Trypanosoma brucei gambiense Tbg972.11.8480   major surface protease A, putative,Gp63 major surface glycoprotein-like protein,MSP-A, putative
Trypanosoma brucei gambiense Tbg972.11.8840   Gp63-1 surface protease homolog, putative
Trypanosoma brucei gambiense Tbg972.8.1230   major surface protease gp63, putative,GP63, putative,metallopeptidase, putative
Trypanosoma brucei Tb927.11.7720   Gp63-1 surface protease homolog, putative
Trypanosoma brucei Tb11.v5.0710   Gp63-1 surface protease homolog, putative
Trypanosoma brucei Tb927.11.7410   MSP-A, putative
Trypanosoma brucei Tb927.11.7760   MSP-A, putative
Trypanosoma brucei Tb927.8.1620   MSP-B, putative
Trypanosoma brucei Tb927.11.7640   Gp63-1 surface protease homolog, putative
Trypanosoma brucei Tb927.11.7630   Gp63-1 surface protease homolog, putative
Trypanosoma brucei Tb927.11.7750   MSP-A, putative
Trypanosoma brucei Tb927.8.1630   MSP-B, putative
Trypanosoma brucei Tb11.v5.0601   major surface protease gp63, putative
Trypanosoma brucei Tb927.8.1640   MSP-B, putative
Trypanosoma brucei Tb11.v5.0511   Gp63-3 surface protease homolog, putative
Trypanosoma brucei Tb11.0380   chrXI additional, unordered contigs
Trypanosoma brucei Tb927.11.7620   MSP-A, putative
Trypanosoma brucei Tb11.v5.0510   Gp63-3 surface protease homolog, putative
Trypanosoma congolense TcIL3000_0_44090   MSP-B, putative
Trypanosoma congolense TcIL3000.11.8250   Major Surface Protease
Trypanosoma congolense TcIL3000_0_06370   Major Surface Protease
Trypanosoma congolense TcIL3000_0_06380   Major Surface Protease
Trypanosoma congolense TcIL3000.11.8010   Major Surface Protease
Trypanosoma cruzi TcCLB.506401.380   surface protease GP63, putative
Trypanosoma cruzi TcCLB.507993.350   surface protease GP63, putative
Trypanosoma cruzi TcCLB.508999.170   surface protease GP63, putative
Trypanosoma cruzi TcCLB.410797.10   surface protease GP63, putative
Trypanosoma cruzi TcCLB.507917.10   surface protease GP63, putative
Trypanosoma cruzi TcCLB.511277.610   surface protease GP63, putative
Trypanosoma cruzi TcCLB.511151.54   surface protease GP63, putative
Trypanosoma cruzi TcCLB.508611.30   surface protease GP63, putative
Trypanosoma cruzi TcCLB.508609.10   surface protease GP63, putative
Trypanosoma cruzi TcCLB.511723.10   surface protease GP63, putative
Trypanosoma cruzi TcCLB.508693.100   surface protease GP63, putative
Trypanosoma cruzi TcCLB.511035.10   surface protease GP63, putative
Trypanosoma cruzi TcCLB.510761.80   surface protease GP63, putative
Trypanosoma cruzi TcCLB.508241.50   surface protease GP63, putative
Trypanosoma cruzi TcCLB.506321.240   surface protease GP63, putative
Trypanosoma cruzi TcCLB.510657.200   surface protease GP63, putative
Trypanosoma cruzi TcCLB.510263.30   surface protease GP63, putative
Trypanosoma cruzi TcCLB.508545.40   surface protease GP63, putative
Trypanosoma cruzi TcCLB.507919.10   surface protease GP63, putative
Trypanosoma cruzi TcCLB.505989.70   surface protease GP63, putative
Trypanosoma cruzi TcCLB.511281.50   surface protease GP63, putative
Trypanosoma cruzi TcCLB.511257.60   surface protease GP63, putative
Trypanosoma cruzi TcCLB.510747.40   surface protease GP63, putative
Trypanosoma cruzi TcCLB.506435.370   surface protease GP63, putative
Trichomonas vaginalis TVAG_295740   Clan MA, family M8, leishmanolysin-like metallopeptidase
Trichomonas vaginalis TVAG_454520   Clan MA, family M8, leishmanolysin-like metallopeptidase
Trichomonas vaginalis TVAG_000880   Clan MA, family M8, leishmanolysin-like metallopeptidase
Trichomonas vaginalis TVAG_195440   Clan MA, family M8, leishmanolysin-like metallopeptidase

Essentiality

Tb927.11.7410 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.1610 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.1610 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.1610 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.1610 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.5610 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.5610 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.5610 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.5610 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.5310 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.5310 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.5310 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.5310 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.5640 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.5640 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.5640 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.5640 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.5630 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.5630 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.5630 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.5630 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major GP63, leishmanolysin Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.11.7410 (Trypanosoma brucei), MSP-A, putative
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