Detailed view for Tb927.4.730

Basic information

TDR Targets ID: 20189
Trypanosoma brucei, Peptidase family C78, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.3437 | Length (AA): 492 | MW (Da): 55017 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07910   Peptidase family C78

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
40 488 5ejj (A) 132 572 32.00 0 1 1.0286 0.98
193 485 5ejj (A) 294 569 41.00 0 1 0.896528 0.11
267 486 2z84 (A) 8 216 33.00 0 1 0.851154 -0.4

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129023)

Species Accession Gene Product
Arabidopsis thaliana AT3G48380   putative Ufm1-specific protease
Babesia bovis BBOV_III009030   protein of unknown function (DUF1671) protein family
Brugia malayi Bm1_39095   hypothetical protein
Caenorhabditis elegans CELE_F38A5.1   Protein F38A5.1, isoform B
Cryptosporidium hominis Chro.60116   CG16979 protein
Cryptosporidium parvum cgd6_900   SPAC25H1.04/CG16979-cterm -like; cysteine protease
Dictyostelium discoideum DDB_G0282953   hypothetical protein
Drosophila melanogaster Dmel_CG16979   CG16979 gene product from transcript CG16979-RA
Drosophila melanogaster Dmel_CG30157   CG30157 gene product from transcript CG30157-RA
Echinococcus granulosus EgrG_000627900   E3 ubiquitin protein ligase listerin
Echinococcus granulosus EgrG_000799700   ufm1 specific protease 1
Echinococcus granulosus EgrG_000586500   Ufm1 specific protease 2
Echinococcus multilocularis EmuJ_000799700   ufm1 specific protease 1
Echinococcus multilocularis EmuJ_000627900   E3 ubiquitin protein ligase listerin
Echinococcus multilocularis EmuJ_000586500   Ufm1 specific protease 2
Homo sapiens 55325   UFM1-specific peptidase 2
Homo sapiens ENSG00000176125   UFM1-specific peptidase 1 (non-functional)
Leishmania braziliensis LbrM.20.3630   hypothetical protein, conserved
Leishmania donovani LdBPK_343830.1   Peptidase family C78, putative
Leishmania infantum LinJ.34.3830   hypothetical protein, conserved
Leishmania major LmjF.34.4000   hypothetical protein, conserved
Leishmania mexicana LmxM.33.4000   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_03930   Ufm1-specific protease 2
Mus musculus ENSMUSG00000051502   UFM1-specific peptidase 1
Mus musculus ENSMUSG00000031634   UFM1-specific peptidase 2
Neospora caninum NCLIV_060120   hypothetical protein, conserved
Oryza sativa 4351994   Os12g0285500
Schistosoma japonicum Sjp_0213530   expressed protein
Schistosoma japonicum Sjp_0082310   Probable Ufm1-specific protease 2, putative
Schistosoma japonicum Sjp_0213520   Zinc finger protein 294, putative
Schistosoma japonicum Sjp_0213540   expressed protein
Schistosoma japonicum Sjp_0308760   IPR012462,Peptidase, ubiquitin fold modifier-specific,domain-containing
Schistosoma japonicum Sjp_0215670   ko:K01376 Peptidase, ubiquitin fold modifier-specific [EC:3.4.22.-], putative
Schistosoma japonicum Sjp_0317800   Ufm1-specific protease 2, putative
Schistosoma japonicum Sjp_0116050   expressed protein
Schistosoma mansoni Smp_179040   UfSP2 peptidase (C78 family)
Schmidtea mediterranea mk4.006398.01   Probable Ufm1-specific protease
Trypanosoma brucei gambiense Tbg972.4.500   hypothetical protein, conserved
Trypanosoma brucei Tb927.4.730   Peptidase family C78, putative
Trypanosoma congolense TcIL3000_0_00270   Peptidase family C78, putative
Trypanosoma cruzi TcCLB.507053.30   Peptidase family C78, putative
Toxoplasma gondii TGME49_216020   peptidase family c78 protein
Theileria parva TP04_0807   hypothetical protein

Essentiality

Tb927.4.730 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.730 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.730 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.730 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.730 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_216020 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.4.730 (Trypanosoma brucei), Peptidase family C78, putative
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