Detailed view for Tb927.11.2280

Basic information

TDR Targets ID: 20209
Trypanosoma brucei, BRO1-like domain/ALIX V-shaped domain binding to HIV, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5352 | Length (AA): 862 | MW (Da): 97080 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03097   BRO1-like domain
PF13949   ALIX V-shaped domain binding to HIV

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

Endocytosis (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
14 738 2xs1 (A) 7 698 20.00 0 1 0.981367 0.09
621 808 4uos (A) 1 183 21.00 0.032 0.96 0.451497 -0.85
644 778 5cwj (A) 2 161 33.00 0.28 1 0.450013 -0.71

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128537)

Species Accession Gene Product
Arabidopsis thaliana AT1G15130   endosomal targeting BRO1-like domain-containing protein
Brugia malayi Bm1_48390   Programmed cell death 6 interacting protein
Candida albicans CaO19.4800   positive regulator of alkaline-induced genes via proteolysis of RIM101 transcription factor
Candida albicans CaO19.12263   positive regulator of alkaline-induced genes via proteolysis of RIM101 transcription factor
Caenorhabditis elegans CELE_R10E12.1   Protein ALX-1, isoform B
Dictyostelium discoideum DDB_G0275451   ALG-2 interacting protein X
Drosophila melanogaster Dmel_CG12876   ALG-2 interacting protein X
Echinococcus granulosus EgrG_000997530   programmed cell death 6 interacting protein
Echinococcus granulosus EgrG_000997550   programmed cell death 6 interacting protein
Entamoeba histolytica EHI_181220   adhesin 112 (EhADH112)
Entamoeba histolytica EHI_165220   hypothetical protein
Echinococcus multilocularis EmuJ_000997530   programmed cell death 6 interacting protein
Echinococcus multilocularis EmuJ_000016600   programmed cell death 6 interacting protein
Echinococcus multilocularis EmuJ_000997550   programmed cell death 6 interacting protein
Homo sapiens ENSG00000170248   programmed cell death 6 interacting protein
Leishmania braziliensis LbrM.27.1770   hypothetical protein, conserved
Leishmania donovani LdBPK_271540.1   BRO1-like domain/ALIX V-shaped domain binding to HIV, putative
Leishmania infantum LinJ.27.1540   hypothetical protein, conserved
Leishmania major LmjF.27.1640   hypothetical protein, conserved
Leishmania mexicana LmxM.27.1640   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05135   hypothetical protein
Loa Loa (eye worm) LOAG_06002   alix-SF
Mus musculus ENSMUSG00000032504   programmed cell death 6 interacting protein
Oryza sativa 4330097   Os02g0638400
Oryza sativa 4348999   Os10g0495300
Saccharomyces cerevisiae YOR275C   Rim20p
Schistosoma japonicum Sjp_0089960   Programmed cell death 6-interacting protein, putative
Schistosoma japonicum Sjp_0054960   Programmed cell death 6-interacting protein, putative
Schistosoma mansoni Smp_087620   programmed cell death protein
Schmidtea mediterranea mk4.016571.00  
Schmidtea mediterranea mk4.020314.00  
Trypanosoma brucei gambiense Tbg972.11.2530   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.2280   BRO1-like domain/ALIX V-shaped domain binding to HIV, putative
Trypanosoma congolense TcIL3000.11.2040   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.503593.30   BRO1-like domain/ALIX V-shaped domain binding to HIV, putative
Trypanosoma cruzi TcCLB.506127.150   BRO1-like domain/ALIX V-shaped domain binding to HIV, putative

Essentiality

Tb927.11.2280 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.18.0002 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.18.0002 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.18.0002 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.18.0002 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.2280 (Trypanosoma brucei), BRO1-like domain/ALIX V-shaped domain binding to HIV, putative
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