Detailed view for PF3D7_1306600

Basic information

TDR Targets ID: 2029
Plasmodium falciparum, V-type proton ATPase subunit H, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.5813 | Length (AA): 455 | MW (Da): 53829 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03224   V-ATPase subunit H
PF11698   V-ATPase subunit H

Gene Ontology

Mouse over links to read term descriptions.
GO:0046961   hydrogen ion transporting ATPase activity, rotational mechanism  
GO:0007035   vacuolar acidification  
GO:0000221   vacuolar proton-transporting V-type ATPase, V1 domain  
GO:0005488   binding  
GO:0015991   ATP hydrolysis coupled proton transport  
GO:0015986   ATP synthesis coupled proton transport  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)
Phagosome (KEGG)

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 419 1ho8 (A) 27 478 20.00 0 1 1.16 -0.94
4 419 1ho8 (A) 27 478 23.00 0 1 1.28 -1.12
26 283 2c1m (A) 239 497 13.00 0.0000000000063 1 0.78 -1.61
11 449 1ho8 (A) 4 478 22.00 0 1 1.12714 0.18
128 332 2bvl (A) 19 250 30.00 0.41 0.3 0.272749 1.37
194 378 4uos (A) 1 182 16.00 0.069 0.91 0.690893 -1.23
11 449 1ho8 (A) 4 478 22.00 0 1 1.13264 0.11
128 332 2bvl (A) 19 250 30.00 0.4 0.42 0.282549 1.4
194 379 4uos (A) 1 183 17.00 0.076 0.69 0.673591 -1.17
339 443 4xvp (D) 10 103 20.00 0.15 0.95 0.393269 -0.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, sporozoite, early ring, early trophozoite, late ring, late trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile early schizont, Oocyst, Sporozoite, Male Gametocyte. PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile gametocyte, Female Gametocyte. PlasmoDB Lasonder E
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_127797)

Species Accession Gene Product
Arabidopsis thaliana AT3G42050   V-type proton ATPase subunit H
Babesia bovis BBOV_III004100   conserved hypothetical protein
Brugia malayi Bm1_04435   hypothetical protein
Caenorhabditis elegans CELE_F52E1.10   Protein VHA-18
Caenorhabditis elegans CELE_T14F9.1   Protein VHA-15
Cryptosporidium hominis Chro.20424   vacuolar ATP synthase subunit h
Cryptosporidium parvum cgd2_3960   vacuolar ATP synthase subunit 54kD
Dictyostelium discoideum DDB_G0274553   vacuolar ATP synthase subunit H
Drosophila melanogaster Dmel_CG17332   Vacuolar H[+]-ATPase SFD subunit
Echinococcus granulosus EgrG_000339600   Vacuolar H ATPase SFD subunit
Entamoeba histolytica EHI_068750   vacuolar ATP synthase subunit H, putative
Echinococcus multilocularis EmuJ_000339600   Vacuolar H+ ATPase SFD subunit
Giardia lamblia GL50803_14961   Vacuolar ATP synthase subunit H
Homo sapiens ENSG00000047249   ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
Leishmania braziliensis LbrM.21.1580   ATP synthase, putative
Leishmania donovani LdBPK_211590.1   ATP synthase, putative
Leishmania infantum LinJ.21.1590   ATP synthase, putative
Leishmania major LmjF.21.1340   ATP synthase, putative
Leishmania mexicana LmxM.21.1340   ATP synthase, putative
Loa Loa (eye worm) LOAG_03309   vacuolar h ATPase 15
Mus musculus ENSMUSG00000033793   ATPase, H+ transporting, lysosomal V1 subunit H
Neospora caninum NCLIV_003220   vacuolar ATP synthase subunit h, putative
Oryza sativa 9272111   Os07g0549700
Plasmodium berghei PBANKA_1405100   V-type proton ATPase subunit H, putative
Plasmodium falciparum PF3D7_1306600   V-type proton ATPase subunit H, putative
Plasmodium knowlesi PKNH_1406800   V-type proton ATPase subunit H, putative
Plasmodium vivax PVX_122165   hypothetical protein, conserved
Plasmodium yoelii PY02592   Drosophila melanogaster SD07421p, putative
Saccharomyces cerevisiae YPR036W   H(+)-transporting V1 sector ATPase subunit H
Schistosoma japonicum Sjp_0301850   ko:K02144 V-type H+-transporting ATPase 54 kD subunit, putative
Schistosoma mansoni Smp_117810   vacuolar ATP synthase subunit h
Schistosoma mansoni Smp_015070.2   vacuolar ATP synthase subunit h
Schistosoma mansoni Smp_015070.1   vacuolar ATP synthase subunit h
Schmidtea mediterranea mk4.001274.06  
Trypanosoma brucei gambiense Tbg972.10.750   ATP synthase, putative
Trypanosoma brucei Tb927.10.730   ATP synthase, putative
Trypanosoma brucei Tb11.v5.0822   ATP synthase, putative
Trypanosoma congolense TcIL3000_10_590   ATP synthase, putative
Trypanosoma cruzi TcCLB.506855.80   ATP synthase, putative
Trypanosoma cruzi TcCLB.508781.20   ATP synthase, putative
Toxoplasma gondii TGME49_208590   vacuolar ATP synthase subunit 54kD, putative
Theileria parva TP03_0033   vacuolar ATP synthase subunit H, putative
Trichomonas vaginalis TVAG_262750   vacuolar ATP synthase subunit H, putative

Essentiality

PF3D7_1306600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.730 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.730 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.730 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.730 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F52E1.10 Caenorhabditis elegans sterile wormbase
CELE_T14F9.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_T14F9.1 Caenorhabditis elegans larval arrest wormbase
CELE_T14F9.1 Caenorhabditis elegans larval lethal wormbase
CELE_T14F9.1 Caenorhabditis elegans slow growth wormbase
PBANKA_1405100 Plasmodium berghei Essential plasmo
TGME49_208590 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

11 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1306600 (Plasmodium falciparum), V-type proton ATPase subunit H, putative
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