Detailed view for PF3D7_1307200

Basic information

TDR Targets ID: 2031
Plasmodium falciparum, DnaJ protein, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 5.7871 | Length (AA): 322 | MW (Da): 38913 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00226   DnaJ domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0031072   heat shock protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
57 161 1nz6 (A) 1 98 18.00 0 0.85 0.17 -0.5
86 170 2cug (A) 3 87 26.00 0 1 0.52 -0.57
99 170 1hdj () 1 72 26.00 0 0.9 0.71 -1.51
3 319 3sfv (B) 237 561 13.00 0.18 0.13 1.00927 0.34
91 155 2ys8 (A) 20 82 35.00 0.17 1 0.688263 -0.81
103 165 2dn9 (A) 10 72 40.00 0.43 0.93 0.699052 -0.55
212 314 4mh6 (A) 44 146 14.00 0.17 0 0.576676 -1.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile gametocyte, Female Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, late ring, Oocyst, Ring, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 16 hs, early schizont, early trophozoite, late trophozoite, Sporozoite. Otto TD PlasmoDB Zanghi G
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_128636)

Species Accession Gene Product
Arabidopsis thaliana AT5G22080   Chaperone DnaJ-domain superfamily protein
Babesia bovis BBOV_III004020   dnaJ domain containing protein
Brugia malayi Bm1_28385   DnaJ domain containing protein
Candida albicans CaO19.2542   DnaJ-like protein similar to S. pombe putative splicing factor spf31 (SPBC1734.05c)
Candida albicans CaO19.10076   DnaJ-like protein similar to S. pombe putative splicing factor spf31 (SPBC1734.05c)
Caenorhabditis elegans CELE_Y71F9B.16   Protein DNJ-30
Cryptosporidium hominis Chro.80057   DNAJ-like protein
Cryptosporidium parvum cgd8_450   DNAj-like protein
Drosophila melanogaster Dmel_CG10375   CG10375 gene product from transcript CG10375-RA
Echinococcus granulosus EgrG_000631900   dnaj subfamily c
Entamoeba histolytica EHI_183050   DnaJ domain containing protein
Echinococcus multilocularis EmuJ_000631900   dnaj subfamily c
Homo sapiens ENSG00000126698   DnaJ (Hsp40) homolog, subfamily C, member 8
Leishmania donovani LdBPK_080930.1   DnaJ domain containing protein, putative
Leishmania infantum LinJ.08.0930   hypothetical protein, conserved
Leishmania major LmjF.08.0990   hypothetical protein, conserved
Leishmania mexicana LmxM.08.0990   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_07124   hypothetical protein
Mus musculus ENSMUSG00000054405   DnaJ (Hsp40) homolog, subfamily C, member 8
Neospora caninum NCLIV_014920   hypothetical protein
Oryza sativa 4332320   Os03g0261500
Plasmodium berghei PBANKA_1405700   DnaJ protein, putative
Plasmodium falciparum PF3D7_1307200   DnaJ protein, putative
Plasmodium knowlesi PKNH_1407500   DnaJ protein, putative
Plasmodium vivax PVX_122195   DnaJ protein, putative
Plasmodium yoelii PY05609   DnaJ domain, putative
Plasmodium yoelii PY00038   SPF31, putative
Schistosoma japonicum Sjp_0014880   ko:K09528 DnaJ homolog, subfamily C, member 8, putative
Schistosoma mansoni Smp_043970   DNAj homolog subfamily C member
Schmidtea mediterranea mk4.000409.03   DnaJ homolog subfamily C member 8
Trypanosoma brucei gambiense Tbg972.5.4050   chaperone protein DNAj, putative
Trypanosoma brucei Tb927.5.2880   chaperone protein DnaJ, putative
Trypanosoma congolense TcIL3000_0_56940   chaperone protein DnaJ, putative
Trypanosoma congolense TcIL3000_5_3140   chaperone protein DnaJ, putative
Trypanosoma cruzi TcCLB.507807.20   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507063.180   hypothetical protein, conserved

Essentiality

PF3D7_1307200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.2880 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.2880 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.2880 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.5.2880 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y71F9B.16 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_1405700 Plasmodium berghei Essential plasmo
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Pkno006337AAT;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Pkno006337; Recombinant protein: truncated; Source: P knowlesi; DNAJ protein, putative homolog ; Identifier: PKH_140610

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_1307200 (Plasmodium falciparum), DnaJ protein, putative
Title for this comment
Comment