Detailed view for Tb927.9.6720

Basic information

TDR Targets ID: 20377
Trypanosoma brucei, Putative methyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.4147 | Length (AA): 338 | MW (Da): 37267 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF10294   Lysine methyltransferase

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 337 4rfq (A) 72 369 27.00 0 1 1.0248 0.88
150 214 4lg1 (A) 56 119 41.00 0.083 0.39 0.411708 0.73
154 202 1wy7 (A) 46 93 35.00 0.3 0.44 0.47937 0.33

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128481)

Species Accession Gene Product
Arabidopsis thaliana AT4G14000   putative methyltransferase family protein
Babesia bovis BBOV_I003320   conserved hypothetical protein
Brugia malayi Bm1_45335   CG17219-PA
Candida albicans CaO19.12224   similar to S. cerevisiae YIL110W
Candida albicans CaO19.4760   similar to S. cerevisiae YIL110W
Caenorhabditis elegans CELE_K01A11.2   Protein K01A11.2
Dictyostelium discoideum DDB_G0270580   UPF0558 protein
Drosophila melanogaster Dmel_CG17219   CG17219 gene product from transcript CG17219-RA
Echinococcus granulosus EgrG_000450700   histidine protein methyltransferase 1
Entamoeba histolytica EHI_152400   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000450700   histidine protein methyltransferase 1
Homo sapiens ENSG00000171806   methyltransferase like 18
Leishmania braziliensis LbrM.15.0830   hypothetical protein, conserved
Leishmania donovani LdBPK_150770.1   Putative methyltransferase, putative
Leishmania infantum LinJ.15.0770   hypothetical protein, conserved
Leishmania major LmjF.15.0730   hypothetical protein, conserved
Leishmania mexicana LmxM.15.0730   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_12598   hypothetical protein
Mus musculus ENSMUSG00000041396   methyltransferase like 18
Neospora caninum NCLIV_055970   Chromosome 1 open reading frame 156, isoform CRA_a, related
Oryza sativa 4346844   Os09g0359900
Onchocerca volvulus OVOC8418  
Plasmodium berghei PBANKA_0506300   conserved Plasmodium protein, unknown function
Plasmodium falciparum PF3D7_1022100   conserved Plasmodium protein, unknown function
Plasmodium knowlesi PKNH_0606400   conserved Plasmodium protein, unknown function
Plasmodium vivax PVX_001970   hypothetical protein, conserved
Plasmodium yoelii PY05046   hypothetical protein
Saccharomyces cerevisiae YIL110W   Hpm1p
Schistosoma japonicum Sjp_0212380   similar to UPF0558 protein C1orf156 homolog, putative
Schistosoma japonicum Sjp_0308730   expressed protein
Schistosoma mansoni Smp_171890.1   hypothetical protein
Schistosoma mansoni Smp_171890.2   hypothetical protein
Schmidtea mediterranea mk4.000799.04  
Trypanosoma brucei gambiense Tbg972.9.3550   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.6720   Putative methyltransferase, putative
Trypanosoma congolense TcIL3000_0_08050   Putative methyltransferase, putative
Trypanosoma cruzi TcCLB.506511.70   Putative methyltransferase, putative
Toxoplasma gondii TGME49_312410   hypothetical protein
Trichomonas vaginalis TVAG_490140   conserved hypothetical protein

Essentiality

Tb927.9.6720 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.4920 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.4920 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.4920 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.4920 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0506300 Plasmodium berghei Dispensable plasmo
TGME49_312410 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.9.6720 (Trypanosoma brucei), Putative methyltransferase, putative
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