Detailed view for Tb927.10.13950

Basic information

TDR Targets ID: 20431
Trypanosoma brucei, tubulin-specific chaperone, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.9641 | Length (AA): 232 | MW (Da): 26460 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01302   CAP-Gly domain
PF14560   Ubiquitin-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

PDB tag
  • 4B6M:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4B6W:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128678)

Species Accession Gene Product
Arabidopsis thaliana AT3G10220   tubulin-binding cofactor B
Babesia bovis BBOV_IV005610   tubulin-specific chaperone, putative
Brugia malayi Bm1_50940   CAP-Gly domain containing protein
Candida albicans CaO19.2828   similar to S. cerevisiae ALF1 (YNL148C) alpha tubulin folding cofactor B co-chaperone
Caenorhabditis elegans CELE_F53F4.3   Protein F53F4.3
Dictyostelium discoideum DDB_G0277983   tubulin folding cofactor B
Drosophila melanogaster Dmel_CG11242   tubulin-binding cofactor B
Echinococcus granulosus EgrG_000526900   Cytoskeleton associated protein CAP Gly
Echinococcus multilocularis EmuJ_000526900   Cytoskeleton associated protein, CAP Gly
Giardia lamblia GL50803_5374   Tubulin specific chaperone B
Homo sapiens ENSG00000105254   tubulin folding cofactor B
Leishmania braziliensis LbrM.18.0520   tubulin-specific chaperone, putative
Leishmania donovani LdBPK_180460.1   tubulin-specific chaperone, putative
Leishmania infantum LinJ.18.0460   tubulin-specific chaperone, putative
Leishmania major LmjF.18.0460   tubulin-specific chaperone, putative
Leishmania mexicana LmxM.18.0460   tubulin-specific chaperone, putative
Loa Loa (eye worm) LOAG_05198   hypothetical protein
Mus musculus ENSMUSG00000006095   tubulin folding cofactor B
Neospora caninum NCLIV_001310   tubulin-specific chaperone, putative
Oryza sativa 4337511   Os04g0692100
Plasmodium berghei PBANKA_0808380   conserved Plasmodium protein, unknown function
Plasmodium berghei PBANKA_0808420   tubulin-specific chaperone, putative
Plasmodium falciparum PF3D7_0906890   conserved Plasmodium protein, unknown function
Plasmodium falciparum PF3D7_0906910   tubulin-specific chaperone, putative
Plasmodium knowlesi PKNH_0704600   conserved Plasmodium protein, unknown function
Plasmodium knowlesi PKNH_0704700   tubulin-specific chaperone, putative
Plasmodium vivax PVX_098786   tubulin-specific chaperone, putative
Plasmodium vivax PVX_098784   hypothetical protein
Plasmodium yoelii PY05144   microtubule-associated protein, putative
Saccharomyces cerevisiae YNL148C   Alf1p
Schistosoma mansoni Smp_055400   tubulin-specific chaperone B (tubulin folding cofactor B)
Schmidtea mediterranea mk4.000246.16   Putative tubulin-specific chaperone B
Trypanosoma brucei gambiense Tbg972.10.17140   tubulin-specific chaperone, putative
Trypanosoma brucei Tb927.10.13950   tubulin-specific chaperone, putative
Trypanosoma congolense TcIL3000_10_11740   tubulin-specific chaperone, putative
Trypanosoma cruzi TcCLB.511277.350   tubulin-specific chaperone, putative
Toxoplasma gondii TGME49_305060   CAP-Gly domain-containing protein
Trichomonas vaginalis TVAG_341080   tubulin-specific chaperone B, putative

Essentiality

Tb927.10.13950 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.13950 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.13950 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.13950 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.13950 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_305060 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.13950 (Trypanosoma brucei), tubulin-specific chaperone, putative
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