Detailed view for Tb927.11.1890

Basic information

TDR Targets ID: 20547
Trypanosoma brucei, Serine-threonine kinase receptor-associated protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2174 | Length (AA): 326 | MW (Da): 36025 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0003674   molecular_function  
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0005515   protein binding  

Metabolic Pathways

RNA transport (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 313 5i2t (A) 123 500 25.00 0.000011 1 0.933415 1.03
35 117 5gxi (A) 633 716 16.00 0 0.07 0.475901 -0.89
38 318 3sfz (A) 614 914 26.00 0 1 1.09786 0.08
247 313 4l9o (B) 2021 2093 37.00 0.72 0.52 0.534421 0.07
253 313 5k19 (A) 227 289 31.00 0.052 0.43 0.521017 -0.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129553)

Species Accession Gene Product
Arabidopsis thaliana AT1G15470   transducin/WD40 repeat-like superfamily protein
Arabidopsis thaliana AT1G52730   transducin/WD-40 repeat-containing protein
Arabidopsis thaliana AT3G15610   transducin/WD40 domain-containing protein
Dictyostelium discoideum DDB_G0286457   WD40 repeat-containing protein
Drosophila melanogaster Dmel_CG3957   wing morphogenesis defect
Echinococcus granulosus EgrG_000622200   serine threonine kinase receptor associated
Echinococcus multilocularis EmuJ_000622200   serine threonine kinase receptor associated
Homo sapiens ENSG00000023734   serine/threonine kinase receptor associated protein
Leishmania braziliensis LbrM.27.1360   hypothetical protein, conserved
Leishmania donovani LdBPK_271140.1   Serine-threonine kinase receptor-associated protein, putative
Leishmania infantum LinJ.27.1140   hypothetical protein, conserved
Leishmania major LmjF.27.1250   hypothetical protein, conserved
Leishmania mexicana LmxM.27.1250   hypothetical protein, conserved
Mus musculus ENSMUSG00000030224   serine/threonine kinase receptor associated protein
Oryza sativa 4341446   Os06g0598900
Oryza sativa 4328675   Os02g0205400
Schistosoma japonicum Sjp_0216730   Serine-threonine kinase receptor-associated protein, putative
Schistosoma mansoni Smp_174390   serine-threonine kinase receptor-associated protein (strap)
Schmidtea mediterranea mk4.003038.00  
Trypanosoma brucei gambiense Tbg972.11.2020   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.1890   Serine-threonine kinase receptor-associated protein, putative
Trypanosoma congolense TcIL3000.11.1580   Serine-threonine kinase receptor-associated protein, putative
Trypanosoma cruzi TcCLB.508357.100   WD domain, G-beta repeat, putative

Essentiality

Tb927.11.1890 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.42.0004 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.42.0004 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.42.0004 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.42.0004 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.11.1890 (Trypanosoma brucei), Serine-threonine kinase receptor-associated protein, putative
Title for this comment
Comment