Detailed view for Tb927.8.5740

Basic information

TDR Targets ID: 20571
Trypanosoma brucei, proteasome 26S non-ATPase subunit 9, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.9788 | Length (AA): 222 | MW (Da): 25105 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0070682   GO:proteasome regulatory particle assembly  

GO:0005838   proteasome regulatory particle  
GO:0005515   protein binding  
GO:0004175   endopeptidase activity  
GO:0043161   proteasomal ubiquitin-dependent protein catabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 96 3whj (A) 30 117 24.00 0.089 0.4 0.678696 -0.79
112 219 4o06 (A) 115 220 23.00 0.0000013 0.58 0.752786 -0.55
123 214 4rey (A) 108 192 35.00 0.0066 0.99 0.680014 0.27

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128010)

Species Accession Gene Product
Arabidopsis thaliana AT5G57950   26S proteasome non-ATPase regulatory subunit 9
Babesia bovis BBOV_II004550   conserved hypothetical protein
Brugia malayi Bm1_11015   Probable 26S proteasome non-ATPase regulatory subunit 9
Brugia malayi Bm1_10245   Probable 26S proteasome non-ATPase regulatory subunit 9
Brugia malayi Bm1_23495   Probable 26S proteasome non-ATPase regulatory subunit 9
Candida albicans CaO19.9837   similar to human proteosomal modulator subunit p27
Candida albicans CaO19.1014   similar to human proteosomal modulator subunit p27
Candida albicans CaO19.2301   similar to human proteosomal modulator subunit p27
Cryptosporidium hominis Chro.50142   proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
Cryptosporidium parvum cgd5_2360   p27 like 26S proteasomal subunit with a PDZ domain
Dictyostelium discoideum DDB_G0275753   26S proteasome non-ATPase regulatory subunit 9
Drosophila melanogaster Dmel_CG9588   CG9588 gene product from transcript CG9588-RA
Echinococcus granulosus EgrG_000982600   26S proteasome non ATPase regulatory subunit
Entamoeba histolytica EHI_074770   proteasome regulatory subunit, putative
Echinococcus multilocularis EmuJ_000982600   26S proteasome non ATPase regulatory subunit
Giardia lamblia GL50803_6647   Hypothetical protein
Homo sapiens ENSG00000110801   proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
Leishmania braziliensis LbrM.16.0290   proteasome 26S non-ATPase subunit 9, putative
Leishmania donovani LdBPK_160300.1   proteasome 26S non-ATPase subunit 9, putative
Leishmania infantum LinJ.16.0300   proteasome 26S non-ATPase subunit 9, putative
Leishmania major LmjF.16.0290   proteasome 26S non-ATPase subunit 9, putative
Leishmania mexicana LmxM.16.0290   proteasome 26S non-ATPase subunit 9, putative
Loa Loa (eye worm) LOAG_04601   hypothetical protein
Mus musculus ENSMUSG00000029440   proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
Neospora caninum NCLIV_047130   hypothetical protein
Oryza sativa 4347083   Os09g0420600
Plasmodium berghei PBANKA_0807400   26S proteasome non-ATPase regulatory subunit 9, putative
Plasmodium falciparum PF3D7_0317800   26S proteasome non-ATPase regulatory subunit 9, putative
Plasmodium knowlesi PKNH_0824000   26S proteasome non-ATPase regulatory subunit 9, putative
Plasmodium vivax PVX_095340   26S proteasome regulatory subunit p27, putative
Plasmodium yoelii PY02649   PDZ domain, putative
Saccharomyces cerevisiae YIL007C   Nas2p
Schistosoma japonicum Sjp_0311100   ko:K06693 26S proteasome non-ATPase regulatory subunit 9, putative
Schistosoma japonicum Sjp_0301990   expressed protein
Schistosoma mansoni Smp_181380   26S proteasome non-ATPase regulatory subunit
Schmidtea mediterranea mk4.000039.02   Probable 26S proteasome non-ATPase regulatory subunit 9
Trypanosoma brucei gambiense Tbg972.8.5740   proteasome 26S non-ATPase subunit 9, putative
Trypanosoma brucei Tb927.8.5740   proteasome 26S non-ATPase subunit 9, putative
Trypanosoma congolense TcIL3000_8_5530   proteasome 26S non-ATPase subunit 9, putative
Trypanosoma cruzi TcCLB.503473.4   proteasome 26S non-ATPase subunit 9, putative
Trypanosoma cruzi TcCLB.503851.54   proteasome 26S non-ATPase subunit 9, putative
Trypanosoma cruzi TcCLB.506743.150   proteasome 26S non-ATPase subunit 9, putative
Toxoplasma gondii TGME49_225580   proteasome (prosome, macropain) 26S subunit, non-ATPase, 9, putative
Theileria parva TP02_0299   hypothetical protein, conserved
Trichomonas vaginalis TVAG_468020   26S proteasome non-ATPase regulatory subunit, putative

Essentiality

Tb927.8.5740 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5740 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.5740 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.5740 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.5740 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_225580 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tbru017718AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru017718; Recombinant protein: full-length; Source: T brucei; proteasome 26S non-ATPase subunit 9, putative ;

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.8.5740 (Trypanosoma brucei), proteasome 26S non-ATPase subunit 9, putative
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