Detailed view for Tb427tmp.55.0014

Basic information

TDR Targets ID: 20605
Trypanosoma brucei, vesicular transport protein (CDC48 homologue), putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.7114 | Length (AA): 706 | MW (Da): 78554 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 1

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)
PF09336   Vps4 C terminal oligomerisation domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0000160   two-component signal transduction system (phosphorelay)  
GO:0006355   regulation of transcription, DNA-dependent  
GO:0005575   cellular_component  
GO:0017111   nucleoside-triphosphatase activity  
GO:0016887   ATPase activity  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  

Metabolic Pathways

Ribosome biogenesis in eukaryotes (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128467)

Species Accession Gene Product
Arabidopsis thaliana AT3G01610   cell division cycle 48C protein
Babesia bovis BBOV_IV011460   ATPase AAA type domain containing protein
Brugia malayi Bm1_47170   ATPase, AAA family protein
Candida albicans CaO19.11695   Member of the AAA family of ATPases
Candida albicans CaO19.4219   Member of the AAA family of ATPases
Caenorhabditis elegans CELE_Y48C3A.7   Protein MAC-1
Cryptosporidium hominis Chro.50178   AAA ATPase
Cryptosporidium parvum cgd5_2010   nuclear VCP like protein with 2 AAA ATpase domains
Dictyostelium discoideum DDB_G0282181   AAA ATPase domain-containing protein
Drosophila melanogaster Dmel_CG8571   smallminded
Entamoeba histolytica EHI_091060   ATPase, AAA family protein
Giardia lamblia GL50803_42442   Transitional endoplasmic reticulum ATPase
Homo sapiens ENSG00000143748   nuclear VCP-like
Leishmania braziliensis LbrM.27.1140   vesicular transport protein (CDC48 homologue), putative
Leishmania donovani LdBPK_270920.1   vesicular transport protein (CDC48 homologue), putative
Leishmania infantum LinJ.27.0920   vesicular transport protein (CDC48 homologue), putative
Leishmania major LmjF.27.1050   vesicular transport protein (CDC48 homologue), putative
Leishmania mexicana LmxM.27.1050   vesicular transport protein (CDC48 homologue), putative
Loa Loa (eye worm) LOAG_07685   ATPase
Mus musculus 67459   nuclear VCP-like
Neospora caninum NCLIV_031210   ATPase, AAA family domain-containing protein, putative
Oryza sativa 4339880   Os06g0109400
Plasmodium berghei PBANKA_1221600   cell division cycle ATPase, putative
Plasmodium falciparum PF3D7_0711000   AAA family ATPase, CDC48 subfamily
Plasmodium knowlesi PKNH_0109700   cell division cycle ATPase, putative
Plasmodium yoelii PY05787   putative cell division cycle ATPase
Saccharomyces cerevisiae YLL034C   putative AAA family ATPase RIX7
Schmidtea mediterranea mk4.048964.00  
Trypanosoma brucei gambiense Tbg972.11.1760   vesicular transport protein (CDC48 homologue), putative
Trypanosoma brucei Tb427tmp.55.0014   vesicular transport protein (CDC48 homologue), putative
Trypanosoma brucei Tb927.11.1660   vesicular transport protein (CDC48 homologue), putative
Trypanosoma congolense TcIL3000.11.1440   vesicular transport protein (CDC48 homologue), putative
Trypanosoma cruzi TcCLB.506629.80   vesicular transport protein (CDC48 homologue), putative
Theileria parva TP01_1158   AAA family ATPase, putative

Essentiality

Tb427tmp.55.0014 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.55.0014 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.55.0014 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.55.0014 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.55.0014 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y48C3A.7 Caenorhabditis elegans larval arrest wormbase
CELE_Y48C3A.7 Caenorhabditis elegans sterile wormbase
YLL034C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1221600 Plasmodium berghei Essential plasmo
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb427tmp.55.0014 (Trypanosoma brucei), vesicular transport protein (CDC48 homologue), putative
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