Detailed view for Tb927.10.14880

Basic information

TDR Targets ID: 20615
Trypanosoma brucei, centrosomal protein of 104 kDa
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6525 | Length (AA): 850 | MW (Da): 92734 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02151   UvrB/uvrC motif

Gene Ontology

Mouse over links to read term descriptions.
GO:0004518   nuclease activity  
GO:0003677   DNA binding  
GO:0005515   protein binding  
GO:0005488   binding  
GO:0006289   nucleotide-excision repair  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
469 720 5lph (A) 416 673 17.00 0 1 0.541924 -0.55
499 609 1m5n (S) 381 483 21.00 0 0.77 0.353908 -0.29
520 712 4k92 (A) 358 537 13.00 0 0.94 0.423222 -0.76
759 889 5lpi (D) 743 875 40.00 0.65 1 0.60438 0.09
853 885 2en6 (A) 9 40 16.00 0.1 0.11 0.126248 0.29
855 897 2n26 (A) 58 99 14.00 0.034 0.06 0.174384 -0.23

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130140)

Species Accession Gene Product
Babesia bovis BBOV_I002130   hypothetical protein
Drosophila melanogaster Dmel_CG10137   CG10137 gene product from transcript CG10137-RA
Echinococcus granulosus EgrG_000243550   centrosomal protein of 104 kDa
Echinococcus multilocularis EmuJ_000243550   hypothetical protein
Giardia lamblia GL50803_16818   Glycine-, glutamate-, thienylcyclohexylpiperidine-binding protein
Homo sapiens ENSG00000116198   centrosomal protein 104kDa
Leishmania braziliensis LbrM.19.0560   hypothetical protein, conserved
Leishmania donovani LdBPK_190240.1   centrosomal protein of 104 kDa
Leishmania infantum LinJ.19.0240   hypothetical protein, conserved
Leishmania major LmjF.19.0250   hypothetical protein, conserved
Leishmania mexicana LmxM.19.0250   hypothetical protein, conserved
Mus musculus ENSMUSG00000039523   centrosomal protein 104
Plasmodium yoelii PY03720   hypothetical protein
Schistosoma mansoni Smp_178710   hypothetical protein
Schmidtea mediterranea mk4.024090.00   Centrosomal protein of 104 kDa
Schmidtea mediterranea mk4.027941.00  
Schmidtea mediterranea mk4.010847.01   Centrosomal protein of 104 kDa
Schmidtea mediterranea mk4.027941.01   Centrosomal protein of 104 kDa
Schmidtea mediterranea mk4.016121.03   Centrosomal protein of 104 kDa
Trypanosoma brucei gambiense Tbg972.10.18050   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.14880   centrosomal protein of 104 kDa
Trypanosoma congolense TcIL3000_10_12740   centrosomal protein of 104 kDa
Trypanosoma cruzi TcCLB.511289.110   centrosomal protein of 104 kDa
Trypanosoma cruzi TcCLB.506211.120   centrosomal protein of 104 kDa
Trichomonas vaginalis TVAG_039890   conserved hypothetical protein
Trichomonas vaginalis TVAG_462570   conserved hypothetical protein
Trichomonas vaginalis TVAG_390050   conserved hypothetical protein
Trichomonas vaginalis TVAG_471010   conserved hypothetical protein
Trichomonas vaginalis TVAG_102530   conserved hypothetical protein

Essentiality

Tb927.10.14880 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.14880 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.14880 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.14880 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.10.14880 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.10.14880 (Trypanosoma brucei), centrosomal protein of 104 kDa
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