Detailed view for Tb927.1.4780

Basic information

TDR Targets ID: 20640
Trypanosoma brucei, Alpha/beta hydrolase domain-containing protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2321 | Length (AA): 670 | MW (Da): 74452 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00326   Prolyl oligopeptidase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0003674   molecular_function  
GO:0008236   serine-type peptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
94 343 5ao9 (A) 7 274 12.00 0.46 0.78 0.428672 0.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127017)

Species Accession Gene Product
Arabidopsis thaliana AT2G24320   alpha/beta-Hydrolases superfamily protein
Arabidopsis thaliana AT4G31020   alpha/beta-Hydrolases superfamily protein
Arabidopsis thaliana AT3G01690   alpha/beta-hydrolase domain-containing protein
Arabidopsis thaliana AT5G38220   alpha/beta-Hydrolases superfamily protein
Arabidopsis thaliana AT1G66900   alpha/beta-Hydrolases superfamily protein
Arabidopsis thaliana AT1G32190   alpha/beta-hydrolase-like protein
Arabidopsis thaliana AT3G30380   alpha/beta-Hydrolases superfamily protein
Arabidopsis thaliana AT5G14390   alpha/beta-hydrolasefamily protein
Arabidopsis thaliana AT4G24760   alpha/beta-Hydrolases superfamily protein
Babesia bovis BBOV_III007870   conserved hypothetical protein
Babesia bovis BBOV_III007880   hypothetical protein
Babesia bovis BBOV_IV005690   hypothetical protein
Brugia malayi Bm1_20405   MGC83647 protein
Caenorhabditis elegans CELE_K04G2.2   Protein AHO-3
Cryptosporidium hominis Chro.60574   similar to CGI-67 protein
Cryptosporidium hominis Chro.40175   hypothetical protein
Cryptosporidium parvum cgd6_4990   predicted peptidase of the alpha/beta-hydrolase fold
Cryptosporidium parvum cgd4_1530   conserved protein
Drosophila melanogaster Dmel_CG33096   CG33096 gene product from transcript CG33096-RG
Echinococcus granulosus EgrG_000958300   Phospholipase carboxylesterase
Entamoeba histolytica EHI_004620   hypothetical protein, conserved
Entamoeba histolytica EHI_115820   hypothetical protein
Echinococcus multilocularis EmuJ_000958300   Phospholipase carboxylesterase
Giardia lamblia GL50803_13896   Cgi67 serine protease precursor-like protein
Homo sapiens ENSG00000107362   abhydrolase domain containing 17B
Homo sapiens ENSG00000136379   abhydrolase domain containing 17C
Homo sapiens 101930551   abhydrolase domain-containing protein FAM108A2/A3-like
Homo sapiens ENSG00000129968   abhydrolase domain containing 17A
Leishmania braziliensis LbrM.20.6000   serine peptidase
Leishmania braziliensis LbrM.33.0390   serine peptidase, putative,serine peptidase, Clan SC, Family S9D
Leishmania donovani LdBPK_330410.1   serine peptidase, putative
Leishmania donovani LdBPK_120920.1   Alpha/beta hydrolase domain-containing protein
Leishmania infantum LinJ.12.0920   serine peptidase, putative,serine peptidase, Clan SC, Family S9D
Leishmania infantum LinJ.33.0410   serine peptidase, putative,serine peptidase, Clan SC, Family S9D
Leishmania major LmjF.33.0400   serine peptidase, putative,serine peptidase, Clan SC, Family S9D
Leishmania major LmjF.12.1330   serine peptidase, putative,serine peptidase, Clan SC, Family S9D
Leishmania mexicana LmxM.32.0400   serine peptidase, putative,serine peptidase, Clan SC, Family S9D
Leishmania mexicana LmxM.12.1330   serine peptidase, putative,serine peptidase, Clan SC, Family S9D
Loa Loa (eye worm) LOAG_03978   hypothetical protein
Loa Loa (eye worm) LOAG_06114   hypothetical protein
Mus musculus ENSMUSG00000038459   abhydrolase domain containing 17C
Mus musculus ENSMUSG00000003346   abhydrolase domain containing 17A
Mus musculus ENSMUSG00000047368   abhydrolase domain containing 17B
Neospora caninum NCLIV_025340   hypothetical protein
Oryza sativa 4331009   Os02g0796600
Oryza sativa 4327946   Os01g0689800
Oryza sativa 4351997   Os12g0286600
Oryza sativa 4344723   Os08g0161500
Oryza sativa 4341592   Os06g0633900
Oryza sativa 4328574   Os02g0190800
Plasmodium berghei PBANKA_1225600   alpha/beta hydrolase, putative
Plasmodium berghei PBANKA_1001500   alpha/beta hydrolase, putative
Plasmodium falciparum PF3D7_0805000   alpha/beta hydrolase, putative
Plasmodium falciparum PF3D7_1120400   alpha/beta hydrolase fold domain containing protein, putative
Plasmodium falciparum PF3D7_0403800   alpha/beta hydrolase, putative
Plasmodium knowlesi PKNH_0918100   alpha/beta hydrolase fold domain containing protein, putative
Plasmodium knowlesi PKNH_0114200   alpha/beta hydrolase, putative
Plasmodium knowlesi PKNH_0301800   alpha/beta hydrolase, putative
Plasmodium vivax PVX_093510   hypothetical protein, conserved
Plasmodium vivax PVX_001055   alpha/beta hydrolase, putative
Plasmodium vivax PVX_091652   hypothetical protein, conserved
Plasmodium yoelii PY02448   hypothetical protein
Schistosoma japonicum Sjp_0116590   Abhydrolase domain-containing protein FAM108C1, putative
Schistosoma japonicum Sjp_0026950   Abhydrolase domain-containing protein FAM108A precursor, putative
Schistosoma japonicum Sjp_0049380   Abhydrolase domain-containing protein FAM108A precursor, putative
Schistosoma japonicum Sjp_0026970   Abhydrolase domain-containing protein FAM108C1, putative
Schistosoma mansoni Smp_027000   family S9 unassigned peptidase (S09 family)
Schistosoma mansoni Smp_038830   peptidase
Schmidtea mediterranea mk4.017318.00  
Schmidtea mediterranea mk4.004184.01   Family S9 unassigned peptidase
Trypanosoma brucei gambiense Tbg972.1.3290   serine peptidase, putative,serine peptidase, Clan SC, Family S9D
Trypanosoma brucei Tb927.1.4780   Alpha/beta hydrolase domain-containing protein
Trypanosoma congolense TcIL3000_1_1980   Alpha/beta hydrolase domain-containing protein
Trypanosoma cruzi TcCLB.507711.330   serine peptidase, putative
Trypanosoma cruzi TcCLB.507997.54   serine peptidase, Clan SC, Family S9D
Trypanosoma cruzi TcCLB.509643.90   serine peptidase, putative
Trypanosoma cruzi TcCLB.508593.44   Alpha/beta hydrolase domain-containing protein
Toxoplasma gondii TGME49_262490   alpha/beta hydrolase, putative
Theileria parva TP03_0361   hypothetical protein, conserved
Theileria parva TP04_0691   hypothetical protein, conserved
Theileria parva TP04_0692   hypothetical protein

Essentiality

Tb927.1.4780 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.4780 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.4780 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.4780 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.4780 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1001500 Plasmodium berghei Slow plasmo
PBANKA_1225600 Plasmodium berghei Dispensable plasmo
TGME49_262490 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) normal (PATO:0000461) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell morphogenesis (GO:0000902) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
regulation of cell motility (GO:0051270) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell cycle (GO:0007049) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
mitochondrion organization and biogenesis (GO:0007005) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
regulation of cell motility (GO:0051270) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
endocytosis (GO:0006897) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.1.4780 (Trypanosoma brucei), Alpha/beta hydrolase domain-containing protein
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