Detailed view for Tb927.9.9200

Basic information

TDR Targets ID: 20671
Trypanosoma brucei, alpha-1,2-mannosyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.5241 | Length (AA): 470 | MW (Da): 52384 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00534   Glycosyl transferases group 1
PF15924   ALG11 mannosyltransferase N-terminus

Gene Ontology

Mouse over links to read term descriptions.
GO:0016757   transferase activity, transferring glycosyl groups  
GO:0005575   cellular_component  
GO:0004377   glycolipid 2-alpha-mannosyltransferase activity  
GO:0005789   endoplasmic reticulum membrane  
GO:0009058   biosynthetic process  

Metabolic Pathways

N-Glycan biosynthesis (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
143 467 4xso (B) 39 378 17.00 0 1 0.848089 0.16
257 448 5i45 (A) 142 328 15.00 0 0.79 0.674111 -0.74
276 433 5d01 (A) 196 339 30.00 0.0019 1 0.69277 -0.72
283 388 4n9w (A) 190 287 34.00 0.48 0.49 0.504132 0.05

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128507)

Species Accession Gene Product
Arabidopsis thaliana AT2G40190   GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Brugia malayi Bm1_22890   glycosyl transferase, group 1 family protein
Candida albicans CaO19.10972   Asn-linked glycosylation, ER-membrane
Candida albicans CaO19.3468   Asn-linked glycosylation, ER-membrane
Caenorhabditis elegans CELE_B0361.8   Protein B0361.8
Cryptosporidium hominis Chro.40340   glycosyl transferase
Cryptosporidium parvum cgd4_2990   glycosyl transferase
Dictyostelium discoideum DDB_G0292118   hypothetical protein
Drosophila melanogaster Dmel_CG11306   CG11306 gene product from transcript CG11306-RB
Echinococcus granulosus EgrG_001082300   GDP Man:Man3GlcNAc2 PP Dol
Entamoeba histolytica EHI_093950   alpha-1,2-mannosyltransferase alg11, putative
Echinococcus multilocularis EmuJ_001082300   GDP Man:Man(3)GlcNAc(2) PP Dol
Homo sapiens ENSG00000253710   ALG11, alpha-1,2-mannosyltransferase
Leishmania braziliensis LbrM.34.5190   alpha-1,2-mannosyltransferase, putative
Leishmania donovani LdBPK_355220.1   glycosyl transferase, putative
Leishmania infantum LinJ.35.5220   alpha-1,2-mannosyltransferase, putative
Leishmania major LmjF.35.5250   alpha-1,2-mannosyltransferase, putative
Leishmania mexicana LmxM.34.5250   glycosyl transferase, putative
Loa Loa (eye worm) LOAG_05679   glycosyl transferase
Mus musculus ENSMUSG00000063362   asparagine-linked glycosylation 11 (alpha-1,2-mannosyltransferase)
Oryza sativa 4352649   Os12g0583000
Onchocerca volvulus OVOC9647   Uncharacterized glycosyltransferase homolog
Saccharomyces cerevisiae YNL048W   alpha-1,2-mannosyltransferase ALG11
Schistosoma japonicum Sjp_0204730   ko:K03844 alpha-1,2-mannosyltransferase, putative
Schistosoma mansoni Smp_052330   glycosyltransferase
Schmidtea mediterranea mk4.002251.00  
Schmidtea mediterranea mk4.000520.03  
Trypanosoma brucei gambiense Tbg972.9.5140   glycosyl transferase, putative
Trypanosoma brucei Tb927.9.9200   alpha-1,2-mannosyltransferase, putative
Trypanosoma congolense TcIL3000_9_3300   glycosyl transferase, putative
Trypanosoma cruzi TcCLB.506885.10   alpha-1,2-mannosyltransferase, putative
Trichomonas vaginalis TVAG_421560   alpha-1,3-mannosyltransferase, putative

Essentiality

Tb927.9.9200 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.0860 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.0860 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.0860 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.0860 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0361.8 Caenorhabditis elegans larval arrest wormbase
CELE_B0361.8 Caenorhabditis elegans slow growth wormbase
CELE_B0361.8 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.9.9200 (Trypanosoma brucei), alpha-1,2-mannosyltransferase, putative
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