Detailed view for PF3D7_1314600

Basic information

TDR Targets ID: 2076
Plasmodium falciparum, lipoate-protein ligase 1
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.0525 | Length (AA): 408 | MW (Da): 47943 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03099   Biotin/lipoate A/B protein ligase family
PF10437   Bacterial lipoate protein ligase C-terminus

Gene Ontology

Mouse over links to read term descriptions.
GO:0016979   lipoate-protein ligase activity  
GO:0003824   catalytic activity  
GO:0009249   protein lipoylation  
GO:0006629   lipid metabolic process  
GO:0006464   protein modification process  

Metabolic Pathways

Lipoic acid metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
19 267 2c8m (A) 1 253 24.00 0 1 0.72 -0.98
24 408 1vqz (A) 3 323 31.00 0 1 0.99 -0.02
19 266 2c8m (A) 1 252 25.00 0 1 0.786443 -0.36
20 408 3a7r (A) 1 336 33.00 0 1 1.15833 0.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 5T8U:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile sporozoite, early ring, early trophozoite. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 48 hs, late ring, late trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, early schizont, Oocyst, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, Sporozoite, Female Gametocyte. Otto TD Zanghi G Lasonder E
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127533)

Species Accession Gene Product
Babesia bovis BBOV_I003930   lipoate-protein ligase A, putative
Brugia malayi Bm1_20445   lipoate-protein ligase, mitochondrial precursor, putative
Candida albicans CaO19.13012   similar to S. cerevisiae YJL046W
Candida albicans CaO19.5566   similar to S. cerevisiae YJL046W
Caenorhabditis elegans CELE_C45G3.3   Protein C45G3.3
Chlamydia trachomatis CT_285   lipoate protein ligase
Dictyostelium discoideum DDB_G0287107   hypothetical protein
Drosophila melanogaster Dmel_CG44243   CG44243 gene product from transcript CG44243-RA
Escherichia coli b4386   lipoate-protein ligase A
Echinococcus granulosus EgrG_000833900   Lipoyltransferase 1 mitochondrial
Echinococcus multilocularis EmuJ_000833900   Lipoyltransferase 1, mitochondrial
Homo sapiens ENSG00000144182   lipoyltransferase 1
Leishmania braziliensis LbrM.07.1130   lipoate-protein ligase-like,lipoyl ligase, putative,lipoyltransferase, putative,lipoate biosynthesis protein, putative
Leishmania donovani LdBPK_071230.1   lipoate-protein ligase-like
Leishmania infantum LinJ.07.1230   lipoate-protein ligase-like,lipoyl ligase, putative,lipoyltransferase, putative,lipoate biosynthesis protein, putative
Leishmania major LmjF.07.1060   lipoate-protein ligase-like,lipoyl ligase, putative,lipoyltransferase, putative,lipoate biosynthesis protein, putative
Leishmania mexicana LmxM.07.1060   lipoate-protein ligase-like,lipoyl ligase, putative,lipoyltransferase, putative,lipoate biosynthesis protein, putative
Mus musculus ENSMUSG00000037216   lipoyltransferase 1
Neospora caninum NCLIV_035300   lipoate-protein ligase a, putative
Onchocerca volvulus OVOC1932   Lipoyltransferase 1, mitochondrial homolog
Plasmodium berghei PBANKA_1413100   lipoate-protein ligase 1
Plasmodium falciparum PF3D7_1314600   lipoate-protein ligase 1
Plasmodium knowlesi PKNH_1415200   lipoate-protein ligase 1, putative
Plasmodium vivax PVX_122550   lipoate-protein ligase 1, putative
Plasmodium yoelii PY00475   probable lipoate-protein ligase a-related
Saccharomyces cerevisiae YJL046W   putative lipoate--protein ligase
Schistosoma japonicum Sjp_0057630   ko:K10105 lipoyltransferase 1, putative
Schistosoma mansoni Smp_086960   lipoate-protein ligase
Schmidtea mediterranea mk4.006463.00   Lipoyltransferase 1, mitochondrial
Schmidtea mediterranea mk4.004477.03   Lipoyltransferase 1, mitochondrial
Trypanosoma brucei gambiense Tbg972.8.230   lipoate-protein ligase, putative,lipoyl ligase, putative,lipoyltransferase, putative,lipoate biosynthesis protein, putative
Trypanosoma brucei Tb927.8.630   lipoyltransferase, putative
Trypanosoma congolense TcIL3000_8_110   lipoyltransferase, putative
Trypanosoma cruzi TcCLB.510857.50   lipoate-protein ligase, putative
Trypanosoma cruzi TcCLB.509611.100   lipoyltransferase, putative
Toxoplasma gondii TGME49_271820   lipoyltransferase and lipoate-protein ligase subfamily protein
Theileria parva TP01_1192   lipoate-protein ligase A, putative
Trichomonas vaginalis TVAG_094820   lipoate-protein ligase, putative

Essentiality

PF3D7_1314600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.630 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.630 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.630 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.630 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b4386 Escherichia coli non-essential goodall
CELE_C45G3.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_C45G3.3 Caenorhabditis elegans larval arrest wormbase
CELE_C45G3.3 Caenorhabditis elegans sterile wormbase
PBANKA_1413100 Plasmodium berghei Essential plasmo
TGME49_271820 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_1314600 (Plasmodium falciparum), lipoate-protein ligase 1
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