Detailed view for LmjF.30.2140

Basic information

TDR Targets ID: 20838
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.6777 | Length (AA): 812 | MW (Da): 87603 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01734   Patatin-like phospholipase
PF11815   Domain of unknown function (DUF3336)

Gene Ontology

Mouse over links to read term descriptions.
GO:0004806   triacylglycerol lipase activity  
GO:0008152   metabolic process  
GO:0006629   lipid metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
450 811 1oxw (A) 26 382 13.00 0 0.71 0.42 -0.68
454 810 1oxw (A) 30 381 13.00 0 0.77 0.48 -0.42
173 268 3gyd (A) 53 155 13.00 0.049 0.21 0.193127 -0.02
432 619 4qmk (B) 25 309 37.00 0.046 0.87 0.249727 0.79

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_129101)

Species Accession Gene Product
Arabidopsis thaliana AT5G04040   triacylglycerol lipase SDP1
Arabidopsis thaliana AT3G57140   sugar-dependent 1-like protein
Candida albicans CaO19.9080   potential patatin-like phospholipase similar to S. pombe SPAC31G5.20c and to S. cerevisiae YOR081C
Candida albicans CaO19.1504   potential patatin-like phospholipase similar to S. pombe SPAC31G5.20c and to S. cerevisiae YOR081C
Candida albicans CaO19.5426   potential patatin-like phospholipase similar to S. cerevisiae YKR089C
Candida albicans CaO19.12881   potential patatin-like phospholipase similar to S. cerevisiae YKR089C
Cryptosporidium hominis Chro.20432   hypothetical protein
Cryptosporidium parvum cgd2_4050   conserved hypothetical protein, signal peptide, 2-3 transmembrane domain at N-terminus
Leishmania braziliensis LbrM.30.2090   hypothetical protein, conserved
Leishmania donovani LdBPK_302150.1   Domain of unknown function (DUF3336)/Patatin-like phospholipase, putative
Leishmania infantum LinJ.30.2150   hypothetical protein, conserved
Leishmania major LmjF.30.2140   hypothetical protein, conserved
Leishmania mexicana LmxM.29.2140   hypothetical protein, conserved
Neospora caninum NCLIV_049240   hypothetical protein
Oryza sativa 4334536   Os03g0810900
Oryza sativa 4327023   Os01g0762000
Plasmodium berghei PBANKA_0824900   patatin-like phospholipase, putative
Plasmodium falciparum PF3D7_0924000   patatin-like phospholipase, putative
Plasmodium knowlesi PKNH_0722000   patatin-like phospholipase, putative
Plasmodium vivax PVX_099615   patatin-like phospholipase, putative
Plasmodium yoelii PY06683   Patatin, putative
Saccharomyces cerevisiae YKR089C   Tgl4p
Saccharomyces cerevisiae YOR081C   Tgl5p
Trypanosoma cruzi TcCLB.509169.20   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511509.50   hypothetical protein, conserved
Toxoplasma gondii TGME49_212130   phospholipase, patatin family protein

Essentiality

LmjF.30.2140 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0824900 Plasmodium berghei Dispensable plasmo
TGME49_212130 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.30.2140 (Leishmania major), hypothetical protein, conserved
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