Detailed view for LmjF.36.6520

Basic information

TDR Targets ID: 20875
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.4846 | Length (AA): 629 | MW (Da): 70138 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
343 412 1yo8 (A) 825 910 34.00 0.37 0.04 0.141088 0.85
411 549 2oto (A) 67 192 22.00 0.084 0 0.224286 0.56
462 616 5cwf (A) 1 155 14.00 0.9 0.03 0.496723 -1.38

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128148)

Species Accession Gene Product
Arabidopsis thaliana AT3G01160   hypothetical protein
Babesia bovis BBOV_III000850   hypothetical protein
Brugia malayi Bm1_12160   ABTAP
Candida albicans CaO19.9855   similar to S. cerevisiae YDR365C
Candida albicans CaO19.2319   similar to S. cerevisiae YDR365C
Caenorhabditis elegans CELE_F58B3.4   Protein F58B3.4
Cryptosporidium hominis Chro.50197   hypothetical protein
Cryptosporidium parvum cgd5_1840   Vir superfamily protein
Dictyostelium discoideum DDB_G0269312   hypothetical protein
Drosophila melanogaster Dmel_CG11417   CG11417 gene product from transcript CG11417-RA
Echinococcus granulosus EgrG_001157300   hypothetical protein
Entamoeba histolytica EHI_097980   NUC153 domain-containing protein
Echinococcus multilocularis EmuJ_001157300   conserved hypothetical protein
Giardia lamblia GL50803_8782   Protein required for cell viability
Homo sapiens ENSG00000089048   ESF1, nucleolar pre-rRNA processing protein, homolog (S. cerevisiae)
Leishmania braziliensis LbrM.35.6860   hypothetical protein, conserved
Leishmania donovani LdBPK_366810.1   hypothetical protein, conserved
Leishmania major LmjF.36.6520   hypothetical protein, conserved
Leishmania mexicana LmxM.36.6520   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_04597   ABTAP
Mus musculus ENSMUSG00000045624   ESF1, nucleolar pre-rRNA processing protein, homolog (S. cerevisiae)
Neospora caninum NCLIV_035720   Predicted CDS Pa_6_6770, related
Oryza sativa 4343456   Os07g0531700
Onchocerca volvulus OVOC11630  
Plasmodium berghei PBANKA_0605700   small subunit rRNA processing factor, putative
Plasmodium falciparum PF3D7_1207100   small subunit rRNA processing factor, putative
Plasmodium knowlesi PKNH_1307100   small subunit rRNA processing factor, putative
Plasmodium vivax PVX_084390   small subunit rRNA processing factor, putative
Plasmodium yoelii PY06370   hypothetical protein
Saccharomyces cerevisiae YDR365C   Esf1p
Schistosoma japonicum Sjp_0203990   ESF1 homolog, putative
Schistosoma mansoni Smp_002230   hypothetical protein
Schmidtea mediterranea mk4.018698.01   ESF1 homolog
Trypanosoma brucei gambiense Tbg972.10.9570   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.7810   Pre-rRNA-processing protein ESF1, putative
Trypanosoma congolense TcIL3000_10_6670   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506247.450   hypothetical protein, conserved
Toxoplasma gondii TGME49_271100   NUC153 domain-containing protein
Theileria parva TP03_0781   hypothetical protein
Theileria parva TP03_0784   hypothetical protein
Theileria parva TP03_0782   hypothetical protein
Theileria parva TP03_0783   hypothetical protein
Trichomonas vaginalis TVAG_462330   conserved hypothetical protein

Essentiality

LmjF.36.6520 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.7810 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.7810 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.7810 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.7810 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F58B3.4 Caenorhabditis elegans larval arrest wormbase
CELE_F58B3.4 Caenorhabditis elegans larval lethal wormbase
CELE_F58B3.4 Caenorhabditis elegans slow growth wormbase
YDR365C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0605700 Plasmodium berghei Essential plasmo
TGME49_271100 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Lmaj006111AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Lmaj006111; Recombinant protein: full-length; Source: L major; hypothetical protein, conserved ;

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier LmjF.36.6520 (Leishmania major), hypothetical protein, conserved
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