Detailed view for LmjF.34.3590

Basic information

TDR Targets ID: 20951
Leishmania major, phosphatidylinositol 4-kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.4252 | Length (AA): 611 | MW (Da): 68795 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00454   Phosphatidylinositol 3- and 4-kinase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016301   kinase activity  
GO:0046854   phosphoinositide phosphorylation  
GO:0048015   phosphoinositide-mediated signaling  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 610 1e7u (A) 563 1094 22.00 0 1 0.81 0.27
258 610 1e7u (A) 732 1094 26.00 0 1 0.89 -0.55
4 610 1e7u (A) 610 1094 23.00 0 1 0.936853 0.45
7 592 5c46 (E) 132 782 30.00 0 1 1.08448 0.03
310 522 5dxu (A) 721 957 35.00 0 1 0.670009 -0.05
321 595 2wxf (A) 748 1027 27.00 0 1 0.744882 -0.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127682)

Species Accession Gene Product
Arabidopsis thaliana AT5G09350   phosphatidylinositol 4-OH kinase beta2
Arabidopsis thaliana AT5G64070   phosphatidylinositol 4-OH kinase beta1
Babesia bovis BBOV_IV009520   phosphatidylinositol 4 kinase, putative
Brugia malayi Bm1_32925   Phosphatidylinositol 3- and 4-kinase family protein
Candida albicans CaO19.10711   phosphatidyl inositol kinase at the alpha mating type-like locus in C. albicans
Candida albicans CaO19.3199   phosphatidyl inositol kinase-like orf at the a mating type-like locus in C. albicans
Caenorhabditis elegans CELE_F35H12.4   Protein PIFK-1
Cryptosporidium hominis Chro.80518   phosphatidylinositol 4-kinase
Cryptosporidium parvum cgd8_4500   phosphatidylinositol 4-kinase
Dictyostelium discoideum DDB_G0286479   phosphatidylinositol 4-kinase
Dictyostelium discoideum DDB_G0288485   phosphatidylinositol 4-kinase
Drosophila melanogaster Dmel_CG7004   four wheel drive
Echinococcus granulosus EgrG_000348000   phosphatidylinositol 4 kinase beta
Echinococcus multilocularis EmuJ_000348000  
Giardia lamblia GL50803_16558   Phosphatidylinositol 4-kinase
Homo sapiens ENSG00000143393   phosphatidylinositol 4-kinase, catalytic, beta
Leishmania braziliensis LbrM.20.3170   phosphatidylinositol 4-kinase, putative
Leishmania donovani LdBPK_343370.1   phosphatidylinositol 4-kinase, putative
Leishmania infantum LinJ.34.3370   phosphatidylinositol 4-kinase, putative
Leishmania major LmjF.34.3590   phosphatidylinositol 4-kinase, putative
Leishmania mexicana LmxM.33.3590   phosphatidylinositol 4-kinase, putative
Loa Loa (eye worm) LOAG_04587   phosphatidylinositol 3
Mus musculus ENSMUSG00000038861   phosphatidylinositol 4-kinase, catalytic, beta polypeptide
Neospora caninum NCLIV_001980   hypothetical protein
Oryza sativa 9267129   Os11g0209700
Plasmodium berghei PBANKA_1109400   phosphatidylinositol 4-kinase, putative
Plasmodium falciparum PF3D7_0509800   phosphatidylinositol 4-kinase
Plasmodium knowlesi PKNH_1023800   phosphatidylinositol 4-kinase, putative
Plasmodium vivax PVX_098050   phosphatidylinositol 4-kinase, putative
Plasmodium yoelii PY04039   phosphatidylinositol 4-kinase-related
Saccharomyces cerevisiae YNL267W   1-phosphatidylinositol 4-kinase
Schistosoma japonicum Sjp_0016410   ko:K00888 phosphatidylinositol 4-kinase [EC2.7.1.67], putative
Schistosoma mansoni Smp_130340   phosphatidylinositol 4-kinase
Schmidtea mediterranea mk4.008159.00   Phosphatidylinositol 4-kinase beta
Schmidtea mediterranea mk4.007900.02   Phosphatidylinositol 4-kinase beta
Schmidtea mediterranea mk4.007900.03   Phosphatidylinositol 4-kinase
Trypanosoma brucei gambiense Tbg972.4.970   phosphatidylinositol 4-kinase, putative
Trypanosoma brucei Tb927.4.1140   phosphatidylinositol 4-kinase, putative
Trypanosoma congolense TcIL3000_4_740   phosphatidylinositol 4-kinase, putative
Trypanosoma cruzi TcCLB.508695.20   phosphatidylinositol 4-kinase, putative
Trypanosoma cruzi TcCLB.505987.70   phosphatidylinositol 4-kinase, putative
Toxoplasma gondii TGME49_296010   phosphatidylinositol 3- and 4-kinase
Toxoplasma gondii TGME49_328200   phosphatidylinositol 4-kinase
Toxoplasma gondii TGME49_295140   1-phosphatidylinositol 4-kinase
Theileria parva TP01_0820   phosphatidylinositol 4-kinase, putative

Essentiality

LmjF.34.3590 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.1140 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.1140 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.1140 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.1140 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YNL267W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1109400 Plasmodium berghei Essential plasmo
TGME49_296010 Toxoplasma gondii Probably non-essential sidik
TGME49_295140 Toxoplasma gondii Probably non-essential sidik
TGME49_328200 Toxoplasma gondii Probably non-essential sidik
TGME49_296010 Toxoplasma gondii Probably essential sidik
TGME49_295140 Toxoplasma gondii Probably essential sidik
TGME49_328200 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens phosphatidylinositol 4-kinase, catalytic, beta Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0743 0.499 1
0.0047 0.4237 0.2767
0.0261 0.2533 0.646
0.0262 0.2521 0.6365
0.1103 0.9302 1
0.0936 0.9336 1
0.091 0.2731 0.877
0.0383 0.4742 1
0.0979 0.9213 1
0.0047 0.4237 0.2767
0.061 0.2961 1
0.065 0.8304 1
0.0617 0.7509 1
0.091 0.2731 0.877
0.091 0.2731 0.877
0.0743 0.499 1
0.0268 0.2524 0.6358
0.086 0.3072 1
0.0741 0.4409 1
0.0864 0.9127 1
0.098 1 1
0.0047 0.4237 0.2767
0.0259 0.2529 0.6362
0.026 0.253 0.6378
0.086 0.3072 1
0.0766 0.8483 1
0.0762 0.873 1
0.0268 0.253 0.6363
0.0126 0.3141 0.0242
0.0261 0.2527 0.6371
0.091 0.2731 0.877
0.0741 0.3851 1
0.0261 0.2533 0.646
0.004 1 0.5
0.078 0.4333 1
0.0617 0.4255 1
0.0047 0.4237 0.2767
0.0264 0.2558 0.6358
0.0901 0.9286 1
0.0617 0.4255 1
0.063 1 1
0.063 1 1
0.078 0.4333 1
0.091 0.2731 0.877
0.0762 0.8727 1
0.0045 0.4237 0.2767
0.0936 0.9336 1
0.091 0.2731 0.877
0.0817 0.4293 1
0.091 0.2731 0.877
0.0033 0.5241 1
0.0607 0.6177 1
0.0109 1 1
0.0936 0.9336 1
0.0253 0.2521 0.637
0.0047 0.4237 0.2767

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.34.3590 (Leishmania major), phosphatidylinositol 4-kinase, putative
Title for this comment
Comment