Detailed view for LmjF.36.3160

Basic information

TDR Targets ID: 20980
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.8612 | Length (AA): 335 | MW (Da): 36818 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07986   Tubulin binding cofactor C

Gene Ontology

Mouse over links to read term descriptions.
GO:0007023   post-chaperonin tubulin folding pathway  
GO:0005488   binding  
GO:0000902   cell morphogenesis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
152 332 2bx6 (A) 55 221 24.00 0 1 0.58 -0.38
2 59 5j0k (A) 16 74 17.00 0.08 0.15 0.441634 -1.64
9 106 2l3l (A) 44 135 30.00 0.023 0.63 0.653337 -0.84
126 271 1k8f (A) 319 461 9.00 0.23 0.12 0.499321 -0.32
158 325 5aj8 (A) 158 325 99.00 0 1 1.77329 -1.88

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 5AJ8:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128787)

Species Accession Gene Product
Arabidopsis thaliana AT4G39920   tubulin-folding cofactor C
Brugia malayi Bm1_53750   hypothetical protein
Caenorhabditis elegans CELE_Y71H2AM.24   Protein Y71H2AM.24
Cryptosporidium hominis Chro.40275   similar to RIKEN cDNA 2810055C19
Cryptosporidium parvum cgd4_2450   conserved hypothetical protein
Dictyostelium discoideum DDB_G0284313   tubulin folding cofactor C
Drosophila melanogaster Dmel_CG31961   CG31961 gene product from transcript CG31961-RA
Echinococcus granulosus EgrG_000413000   tubulin specific chaperone C
Echinococcus multilocularis EmuJ_000413000   tubulin specific chaperone C
Giardia lamblia GL50803_15906   Tubulin specific chaperone D
Homo sapiens ENSG00000124659   tubulin folding cofactor C
Leishmania braziliensis LbrM.35.3380   hypothetical protein, conserved
Leishmania donovani LdBPK_363310.1   tubulin binding cofactor c, putative
Leishmania infantum LinJ.36.3310   hypothetical protein, conserved
Leishmania major LmjF.36.3160   hypothetical protein, conserved
Leishmania mexicana LmxM.36.3160   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_06411   hypothetical protein
Mus musculus ENSMUSG00000036430   tubulin-specific chaperone C
Neospora caninum NCLIV_040280   hypothetical protein, conserved
Plasmodium berghei PBANKA_1214100   tubulin binding cofactor c, putative
Plasmodium falciparum PF3D7_1015700   tubulin binding cofactor c, putative
Plasmodium knowlesi PKNH_0815800   tubulin binding cofactor c, putative
Plasmodium vivax PVX_095005   hypothetical protein
Plasmodium yoelii PY04613   hypothetical protein
Schistosoma japonicum Sjp_0314470   expressed protein
Schistosoma mansoni Smp_097710   hypothetical protein
Schmidtea mediterranea mk4.005301.02   Tubulin-specific chaperone C
Trypanosoma brucei gambiense Tbg972.11.10640   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.9480   tubulin binding cofactor c, putative
Trypanosoma congolense TcIL3000.11.9950   tubulin binding cofactor c, putative
Trypanosoma cruzi TcCLB.508851.140   tubulin binding cofactor c, putative
Trypanosoma cruzi TcCLB.511589.80   tubulin binding cofactor c, putative
Toxoplasma gondii TGME49_264950   hypothetical protein
Theileria parva TP01_1194   hypothetical protein
Trichomonas vaginalis TVAG_091930   tubulin folding cofactor C, putative

Essentiality

LmjF.36.3160 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1240 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.1240 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.1240 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.1240 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1214100 Plasmodium berghei Essential plasmo
TGME49_264950 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.36.3160 (Leishmania major), hypothetical protein, conserved
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