Detailed view for LmjF.14.0350

Basic information

TDR Targets ID: 21178
Leishmania major, threonine synthase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.0426 | Length (AA): 676 | MW (Da): 73523 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00291   Pyridoxal-phosphate dependent enzyme
PF14821   Threonine synthase N terminus

Gene Ontology

Mouse over links to read term descriptions.
GO:0030170   pyridoxal phosphate binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
95 655 1kl7 (A) 3 512 31.00 0 1 1.02 -0.93
103 652 1kl7 (A) 8 512 33.00 0 1 1.13 -0.85
100 639 4f4f (A) 0 460 39.00 0 1 1.13092 -0.22
103 655 1kl7 (A) 8 512 33.00 0 1 1.07615 -0.18

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128662)

Species Accession Gene Product
Candida albicans CaO19.4233   threonine synthase
Candida albicans CaO19.11708   threonine synthase
Escherichia coli b0004   L-threonine synthase
Homo sapiens ENSG00000185875   threonine synthase-like 1 (S. cerevisiae)
Homo sapiens ENSG00000144115   threonine synthase-like 2 (S. cerevisiae)
Leishmania braziliensis LbrM.14.0350   threonine synthase, putative
Leishmania donovani LdBPK_140350.1   threonine synthase, putative
Leishmania infantum LinJ.14.0350   threonine synthase, putative
Leishmania major LmjF.14.0350   threonine synthase, putative
Leishmania mexicana LmxM.14.0350   threonine synthase, putative
Mus musculus ENSMUSG00000048550   threonine synthase-like 1 (bacterial)
Mus musculus ENSMUSG00000054474   threonine synthase-like 2 (bacterial)
Neospora caninum NCLIV_004630   threonine synthase, putative
Saccharomyces cerevisiae YCR053W   threonine synthase THR4
Schmidtea mediterranea mk4.002839.00  
Schmidtea mediterranea mk4.044997.02   Threonine synthase-like 1
Schmidtea mediterranea mk4.000631.16  
Schmidtea mediterranea mk4.007220.00  
Schmidtea mediterranea mk4.003415.00  
Trypanosoma brucei gambiense Tbg972.7.4960   threonine synthase, putative
Trypanosoma brucei Tb927.7.4390   threonine synthase, putative
Trypanosoma congolense TcIL3000_7_3590   threonine synthase, putative
Trypanosoma cruzi TcCLB.504213.80   threonine synthase, putative
Trypanosoma cruzi TcCLB.508857.160   threonine synthase, putative
Toxoplasma gondii TGME49_220840   threonine synthase

Essentiality

LmjF.14.0350 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.4390 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.4390 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.4390 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.4390 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0004 Escherichia coli non-essential goodall
TGME49_220840 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.14.0350 (Leishmania major), threonine synthase, putative
Title for this comment
Comment