Detailed view for LmjF.34.3780

Basic information

TDR Targets ID: 21513
Leishmania major, phosphomannomutase-like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.4325 | Length (AA): 594 | MW (Da): 65236 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00408   Phosphoglucomutase/phosphomannomutase, C-terminal domain
PF02878   Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I
PF02879   Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II
PF02880   Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III

Gene Ontology

Mouse over links to read term descriptions.
GO:0071704   GO:organic substance metabolic process  

GO:0016868   intramolecular transferase activity, phosphotransferases  
GO:0000287   magnesium ion binding  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 577 2fuv (A) 3 546 20.00 0 1 1.07 -0.47
24 575 2fuv (A) 19 544 21.00 0 1 1.15 -0.52
44 455 1tuo (A) 12 383 29.00 0 1 0.99 -0.59
18 575 3na5 (A) 10 544 21.00 0 1 1.04079 0.33
41 575 3na5 (A) 37 544 21.00 0 1 1.05387 0.45
44 455 1tuo (A) 12 383 29.00 0 1 0.903103 0.31
142 194 2dka (B) 59 92 68.00 0.53 0.9 0.174726 1.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127613)

Species Accession Gene Product
Brugia malayi Bm1_18455   Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I family protein
Candida albicans CaO19.6739   similar to phosphomannomutase
Candida albicans CaO19.14031   similar to phosphomannomutase
Caenorhabditis elegans CELE_Y43F4B.5   Protein Y43F4B.5, isoform B
Chlamydia trachomatis CT_295   phosphomannomutase
Dictyostelium discoideum DDB_G0280897   hypothetical protein
Drosophila melanogaster Dmel_CG8073   Phosphomannomutase 45A
Drosophila melanogaster Dmel_CG10202   CG10202 gene product from transcript CG10202-RA
Echinococcus granulosus EgrG_001087500   phosphoglucomutase 2
Entamoeba histolytica EHI_185280   phosphoglucomutase/phosphomannomutase family protein
Echinococcus multilocularis EmuJ_001087500   phosphoglucomutase 2
Giardia lamblia GL50803_17254   Phosphomannomutase-2
Homo sapiens ENSG00000165434   phosphoglucomutase 2-like 1
Homo sapiens ENSG00000169299   phosphoglucomutase 2
Leishmania braziliensis LbrM.20.3380   phosphomannomutase-like protein
Leishmania donovani LdBPK_343580.1   phosphomannomutase-like protein
Leishmania infantum LinJ.34.3580   phosphomannomutase-like protein
Leishmania major LmjF.34.3780   phosphomannomutase-like protein
Leishmania mexicana LmxM.33.3780   phosphomannomutase-like protein
Loa Loa (eye worm) LOAG_03475   hypothetical protein
Loa Loa (eye worm) LOAG_03474   hypothetical protein
Mycobacterium leprae ML0706c   Probable phosphomannomutase PmmB (PHOSPHOMANNOSE MUTASE)
Mus musculus ENSMUSG00000029171   phosphoglucomutase 1
Mus musculus 70974   phosphoglucomutase 2-like 1
Mycobacterium tuberculosis Rv3308   Probable phosphomannomutase PmmB (phosphomannose mutase)
Mycobacterium ulcerans MUL_2674   phosphomannomutase
Neospora caninum NCLIV_010960   phosphoglucomutase, putative
Onchocerca volvulus OVOC7771  
Plasmodium berghei PBANKA_1210900   phosphoglucomutase, putative
Plasmodium falciparum PF3D7_1012500   phosphoglucomutase, putative
Plasmodium knowlesi PKNH_0812300   phosphoglucomutase, putative
Plasmodium vivax PVX_094845   phosphoglucomutase, putative
Plasmodium yoelii PY03478   hypoxanthine phosphoribosyltransferase, putative
Saccharomyces cerevisiae YMR278W   phosphoglucomutase PGM3
Schistosoma japonicum Sjp_0300930   ko:K01840 phosphomannomutase [EC5.4.2.8], putative
Schistosoma mansoni Smp_085150   phosphoglucomutase
Schmidtea mediterranea mk4.004555.00   Putative phosphoglucomutase
Schmidtea mediterranea mk4.002495.01  
Schmidtea mediterranea mk4.002495.00   Putative phosphoglucomutase
Trypanosoma cruzi TcCLB.506405.10   phosphomannomutase-like protein, putative
Trypanosoma cruzi TcCLB.508257.70   phosphomannomutase-like protein, putative
Toxoplasma gondii TGME49_318580   glucosephosphate-mutase GPM2
Treponema pallidum TP0642   phosphomannomutase
Trichomonas vaginalis TVAG_054830   phosphoglucomutase, putative
Trichomonas vaginalis TVAG_300510   phosphoglucomutase, putative
Trichomonas vaginalis TVAG_205910   phosphoglucomutase, putative
Trichomonas vaginalis TVAG_405900   phosphoglucomutase, putative

Essentiality

LmjF.34.3780 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_Y43F4B.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y43F4B.5 Caenorhabditis elegans larval arrest wormbase
CELE_Y43F4B.5 Caenorhabditis elegans larval lethal wormbase
CELE_Y43F4B.5 Caenorhabditis elegans slow growth wormbase
PBANKA_1210900 Plasmodium berghei Dispensable plasmo
TGME49_318580 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.34.3780 (Leishmania major), phosphomannomutase-like protein
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