Detailed view for PF3D7_1333200

Basic information

TDR Targets ID: 2166
Plasmodium falciparum, ubiquitin-activating enzyme
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.2855 | Length (AA): 1838 | MW (Da): 218525 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 3

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00899   ThiF family
PF10585   Ubiquitin-activating enzyme active site

Gene Ontology

Mouse over links to read term descriptions.
GO:0020011   apicoplast  
GO:0016020   membrane  
GO:0008641   small protein activating enzyme activity  
GO:0005524   ATP binding  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  
GO:0006464   protein modification process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 18 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
161 668 1ngv (A) 6 526 15.00 0 1 0.19 -0.43
162 381 1zud (1) 2 238 17.00 0 1 0.14 -1.05
692 974 1ngv (B) 40 233 35.00 0.0000007 0.76 -0.05 1.43
758 1339 1y8q (B) 7 407 24.00 0 0.98 -0.04 0.87
165 1517 4nnj (A) 15 1019 22.00 0 1 0.180726 1.42
174 304 4d79 (A) 18 153 18.00 0.004 1 0.331273 -0.75
1088 1786 3v0a (B) 471 1190 23.00 0.00000012 0.99 0.377405 1.41
1552 1741 4uos (A) 2 184 20.00 0.082 0.85 0.478373 -1.75
1598 1821 4tql (A) 9 235 16.00 0.071 0.25 0.252872 -0.61
1613 1779 5dfz (D) 161 329 29.00 0.23 0.02 -0.0310404 2.13
182 335 1jwa (B) 3 163 22.00 0.000019 0.96 0.335474 -0.25
186 1585 4nnj (A) 15 1023 22.00 0 1 0.14851 1.46
195 325 4d79 (A) 18 153 18.00 0.0031 0.99 0.334882 -0.82
693 993 2nvu (B) 1367 2233 32.00 0.00002 0.79 -0.125023 1.84
712 1018 3dbh (B) 40 258 29.00 0.00000000017 1 -0.0682905 1.77
1508 1837 3ren (A) 50 361 23.00 0.61 0.88 0.261102 0.48
1570 1759 4uos (A) 2 184 20.00 0.052 0.99 0.496671 -1.82
1616 1839 4tql (A) 9 235 17.00 0.067 0.52 0.27999 -0.54

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile sporozoite, Sporozoite. PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile early schizont, early trophozoite, late ring, late trophozoite. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early ring, late schizont, Oocyst. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, gametocyte, Ring, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_146901)

Species Accession Gene Product
Babesia bovis BBOV_III005870   ThiF family protein
Plasmodium berghei PBANKA_1347900   ubiquitin activating enzyme, putative
Plasmodium falciparum PF3D7_1333200   ubiquitin-activating enzyme
Plasmodium knowlesi PKNH_1267400   ubiquitin activating enzyme, putative
Plasmodium vivax PVX_082590   ubiquitin-activating enzyme, putative
Plasmodium yoelii PY01851   ThiF family, putative
Plasmodium yoelii PY06413   ubiquitin-activating enzyme e1 1
Theileria parva TP02_0331   ubiquitin activating enzyme, putatuve

Essentiality

PF3D7_1333200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1347900 Plasmodium berghei Essential plasmo
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1333200 (Plasmodium falciparum), ubiquitin-activating enzyme
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