Detailed view for PF3D7_1360500

Basic information

TDR Targets ID: 218
Plasmodium falciparum, guanylyl cyclase beta
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.7655 | Length (AA): 3179 | MW (Da): 376990 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 21

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00211   Adenylate and Guanylate cyclase catalytic domain
PF16212   Phospholipid-translocating P-type ATPase C-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0035556   GO:intracellular signal transduction  

GO:0016849   phosphorus-oxygen lyase activity  
GO:0009190   cyclic nucleotide biosynthetic process  
GO:0007242   intracellular signaling cascade  

Metabolic Pathways

Purine metabolism (KEGG)

Structural information

Modbase 3D models:

There are 16 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 1198 1wpg (A) 10 993 14.00 0 0.59 -0.06 -0.07
1540 1744 1wc3 (A) 1004 1195 22.00 0 1 0.14 -0.86
2962 3123 1yk9 (A) 245 399 30.00 0 1 0.38 -0.03
2967 3113 1wc3 (A) 1011 1163 24.00 0.000000000023 0.91 0.4 -1.35
92 1195 4hqj (A) 123 1004 18.00 0.0000000061 1 -0.022021 1.82
1917 2104 4uos (A) 3 183 17.00 0.63 0.04 0.226838 -0.81
2958 3120 4ni2 (A) 471 626 32.00 0 0.99 0.427974 -0.55
2959 3154 2wz1 (A) 412 609 23.00 0 1 0.279355 -0.25
2963 3155 3r5g (A) 224 414 20.00 0 1 0.334411 -0.71
92 1211 4hqj (A) 123 1004 18.00 0.000000013 0.82 -0.0156552 1.87
1537 1745 4p2f (A) 240 422 34.00 0.000000000017 1 0.347306 0
1548 1744 3et6 (A) 477 650 37.00 0.00000000015 1 0.345528 -0.12
1867 2038 4uos (A) 17 183 18.00 0.47 0.08 0.239856 -0.81
2955 3117 4ni2 (A) 471 626 32.00 0 0.99 0.422022 -0.55
2956 3151 2wz1 (A) 412 609 23.00 0 1 0.273413 -0.25
2960 3152 3r5g (A) 224 414 20.00 0 1 0.328468 -0.71

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Female Gametocyte, Male Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Sporozoite. Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Oocyst, Ring. Otto TD Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_156251)

Species Accession Gene Product
Plasmodium berghei PBANKA_1136700   guanylyl cyclase beta
Plasmodium falciparum PF3D7_1360500   guanylyl cyclase beta
Plasmodium knowlesi PKNH_1110800   guanylyl cyclase, putative
Plasmodium vivax PVX_114990   guanylyl cyclase, putative
Plasmodium yoelii PY00952   guanylyl cyclase enzyme-related
Plasmodium yoelii PY00951   guanylyl cyclase enzyme-related
Plasmodium yoelii PY02999   guanylyl cyclase enzyme-related

Essentiality

PF3D7_1360500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1136700 Plasmodium berghei Slow plasmo
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

8 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_1360500 (Plasmodium falciparum), guanylyl cyclase beta
Title for this comment
Comment