Detailed view for LmjF.36.4450

Basic information

TDR Targets ID: 21809
Leishmania major, mitochondrial intermediate peptidase, putative,metallo-peptidase, Clan MA(E), Family M3
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5029 | Length (AA): 676 | MW (Da): 76012 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01432   Peptidase family M3

Gene Ontology

Mouse over links to read term descriptions.
GO:0006627   mitochondrial protein processing during import  
GO:0005759   mitochondrial matrix  
GO:0004222   metalloendopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
42 675 1y79 (1) 1 673 18.00 0 1 0.96 -0.51
50 666 1y79 (1) 83 664 20.00 0 1 1.1 -0.4
62 675 1s4b (P) 29 672 21.00 0 1 1 -0.71
42 675 5l44 (A) 11 665 18.00 0 1 0.98517 -0.15
42 675 4ka7 (A) 94 779 17.00 0 1 0.98417 -0.24
47 675 2o3e (A) 15 673 22.00 0 1 1.03777 -0.25
75 675 5l44 (A) 117 665 19.00 0 1 1.04165 0.03
184 619 4ka7 (A) 320 719 28.00 0 1 0.79427 0.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128475)

Species Accession Gene Product
Arabidopsis thaliana AT5G51540   zincin-like metalloproteases family protein
Babesia bovis BBOV_III002610   peptidase family M3 containing protein
Brugia malayi Bm1_11870   Peptidase family M3 containing protein
Candida albicans CaO19.8786   Mitochondrial intermediate peptidase precursor
Candida albicans CaO19.1195   Mitochondrial intermediate peptidase precursor
Caenorhabditis elegans CELE_Y67H2A.7   Protein Y67H2A.7
Dictyostelium discoideum DDB_G0295743   peptidase M3A and M3B domain-containing protein
Drosophila melanogaster Dmel_CG7791   CG7791 gene product from transcript CG7791-RA
Echinococcus granulosus EgrG_000491800   mitochondrial intermediate peptidase
Echinococcus multilocularis EmuJ_000491800   mitochondrial intermediate peptidase
Homo sapiens ENSG00000027001   mitochondrial intermediate peptidase
Leishmania braziliensis LbrM.35.4690   mitochondrial intermediate peptidase, putative,metallo-peptidase, Clan MA(E), Family M3
Leishmania donovani LdBPK_364670.1   mitochondrial intermediate peptidase, putative
Leishmania infantum LinJ.36.4670   mitochondrial intermediate peptidase, putative,metallo-peptidase, Clan MA(E), Family M3
Leishmania major LmjF.36.4450   mitochondrial intermediate peptidase, putative,metallo-peptidase, Clan MA(E), Family M3
Leishmania mexicana LmxM.36.4450   mitochondrial intermediate peptidase, putative,metallo-peptidase, Clan MA(E), Family M3
Loa Loa (eye worm) LOAG_02623   peptidase family M3 containing protein
Mus musculus ENSMUSG00000021993   mitochondrial intermediate peptidase
Neospora caninum NCLIV_034640   peptidase family M3 domain containing protein, putative
Oryza sativa 4341884   Os06g0686500
Onchocerca volvulus OVOC9353   Mitochondrial intermediate peptidase homolog
Plasmodium berghei PBANKA_1350800   mitochondrial intermediate peptidase, putative
Plasmodium falciparum PF3D7_1337000   mitochondrial intermediate peptidase, putative
Plasmodium knowlesi PKNH_1264300   mitochondrial intermediate peptidase, putative
Plasmodium vivax PVX_082780   mitochondrial intermediate peptidase, putative
Plasmodium yoelii PY06253   hypothetical protein
Saccharomyces cerevisiae YKL134C   Oct1p
Schistosoma japonicum Sjp_0131320   ko:K01410 mitochondrial intermediate peptidase [EC3.4.24.59], putative
Schistosoma japonicum Sjp_0209760   ko:K01410 mitochondrial intermediate peptidase [EC3.4.24.59], putative
Schistosoma mansoni Smp_075220.1   mitochondrial intermediate peptidase (M03 family)
Schistosoma mansoni Smp_075220.3   mitochondrial intermediate peptidase (M03 family)
Schistosoma mansoni Smp_075220.2   mitochondrial intermediate peptidase (M03 family)
Schmidtea mediterranea mk4.037705.00   Mitochondrial intermediate peptidase
Schmidtea mediterranea mk4.022534.00   Mitochondrial intermediate peptidase
Schmidtea mediterranea mk4.003170.03  
Schmidtea mediterranea mk4.007791.00   Mitochondrial intermediate peptidase
Trypanosoma brucei gambiense Tbg972.10.11990   mitochondrial intermediate peptidase, putative,metallo-peptidase, Clan MA(E) Family M3
Trypanosoma brucei Tb927.10.9820   mitochondrial intermediate peptidase, putative
Trypanosoma brucei Tb11.v5.0565   mitochondrial intermediate peptidase, putative
Trypanosoma cruzi TcCLB.504147.80   mitochondrial intermediate peptidase, putative
Toxoplasma gondii TGME49_272670   peptidase family M3 protein
Theileria parva TP01_0515   mitochondrial intermediate peptidase, putative

Essentiality

LmjF.36.4450 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.9820 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.9820 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.9820 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.9820 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1350800 Plasmodium berghei Essential plasmo
TGME49_272670 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.36.4450 (Leishmania major), mitochondrial intermediate peptidase, putative,metallo-peptidase, Clan MA(E), Family M3
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