Detailed view for LmjF.34.3120

Basic information

TDR Targets ID: 22289
Leishmania major, lipophosphoglycan biosynthetic protein (lpg2)
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.6756 | Length (AA): 341 | MW (Da): 37265 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 9

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03151   Triose-phosphate Transporter family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0005338   nucleotide-sugar transmembrane transporter activity  
GO:0015780   nucleotide-sugar transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
73 273 2b2h (A) 8 200 12.00 0.0000086 0 0.79 -1.5
224 299 2f2m (A) 30 106 8.00 0.000001 0 0.38 -1.11
42 134 4jos (A) 80 165 31.00 0.31 0.06 0.301527 2.01
77 256 3d31 (C) 62 247 18.00 0 0.04 0.493259 0.95

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127495)

Species Accession Gene Product
Arabidopsis thaliana AT1G07290   GDP-mannose transporter
Arabidopsis thaliana AT2G13650   GDP-mannose transporter GONST1
Arabidopsis thaliana AT4G32272   nucleotide/sugar transporter family protein
Arabidopsis thaliana AT4G31600   UDP-galactose transporter 7
Brugia malayi Bm1_24080   UDP-sugar transporter-like protein
Candida albicans CaO19.1232   essential gene in C. albicans whose partial loss results in mannosylation and cell wall defects
Candida albicans CaO19.8817   essential gene in C. albicans whose partial loss results in mannosylation and cell wall defects
Caenorhabditis elegans CELE_C52E12.3   Protein SQV-7
Dictyostelium discoideum DDB_G0278631   DUF250 family protein
Dictyostelium discoideum DDB_G0276625   hypothetical protein
Drosophila melanogaster Dmel_CG3874   fringe connection
Echinococcus granulosus EgrG_000492100   UDP glucuronic acid:UDP N acetylgalactosamine
Echinococcus multilocularis EmuJ_000492100   UDP glucuronic acid:UDP N acetylgalactosamine
Homo sapiens ENSG00000130958   solute carrier family 35 (UDP-GlcNAc/UDP-glucose transporter), member D2
Homo sapiens ENSG00000116704   solute carrier family 35 (UDP-GlcA/UDP-GalNAc transporter), member D1
Leishmania braziliensis LbrM.20.2700   lipophosphoglycan biosynthetic protein (lpg2)
Leishmania donovani LdBPK_044290.1   hypothetical protein, conserved
Leishmania infantum LinJ.34.4290   lipophosphoglycan biosynthetic protein (lpg2)
Leishmania major LmjF.34.3120   lipophosphoglycan biosynthetic protein (lpg2)
Leishmania mexicana LmxM.33.3120   lipophosphoglycan biosynthetic protein (lpg2)
Loa Loa (eye worm) LOAG_01225   hypothetical protein
Mus musculus 242585   solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1
Mus musculus 102641304   UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose transporter-like
Mus musculus ENSMUSG00000033114   solute carrier family 35, member D2
Oryza sativa 4329973   Os02g0614100
Oryza sativa 4344457   Os08g0107400
Saccharomyces cerevisiae YGL225W   Vrg4p
Saccharomyces cerevisiae YER039C   Hvg1p
Schistosoma japonicum Sjp_0215980   UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter, putative
Schistosoma mansoni Smp_178490   solute carrier family 35 member d1
Schmidtea mediterranea mk4.000392.10   UDP-sugar transporter sqv-7
Trypanosoma brucei gambiense Tbg972.4.1520   lipophosphoglycan biosynthetic protein 2, putative
Trypanosoma brucei Tb927.4.1640   Nucleotide sugar transporter 3
Trypanosoma congolense TcIL3000_4_1220   lipophosphoglycan biosynthetic protein 2, putative
Trypanosoma cruzi TcCLB.506793.40   UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter, putative
Trypanosoma cruzi TcCLB.504057.120   lipophosphoglycan biosynthetic protein 2, putative
Trypanosoma cruzi TcCLB.510611.20   UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter, putative

Essentiality

LmjF.34.3120 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.1640 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.1640 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.1640 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.1640 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C52E12.3 Caenorhabditis elegans embryonic lethal wormbase
YGL225W Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.34.3120 (Leishmania major), lipophosphoglycan biosynthetic protein (lpg2)
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