Detailed view for LmjF.32.0890

Basic information

TDR Targets ID: 22546
Leishmania major, ethanolamine-phosphate cytidylyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.9195 | Length (AA): 402 | MW (Da): 45410 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01467   Cytidylyltransferase-like

Gene Ontology

Mouse over links to read term descriptions.
GO:0016779   nucleotidyltransferase activity  
GO:0003824   catalytic activity  
GO:0009058   biosynthetic process  

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
50 181 1coz (A) 1 126 31.00 0 1 0.69 -1.07
53 246 1mrz (A) 2 217 17.00 0 1 0.57 0.59
28 88 3inn (A) 0 59 22.00 0.0016 0.52 0.355041 0.52
48 367 3elb (A) 20 350 48.00 0 1 1.32332 -0.34
52 170 2b7l (A) 3 115 31.00 0 1 0.70792 -0.72
227 395 4mvd (B) 72 240 31.00 0 1 0.810698 -0.27
362 401 5hmo (A) 808 846 31.00 0.89 0.09 0.561803 -1.7

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127671)

Species Accession Gene Product
Arabidopsis thaliana AT2G38670   ethanolamine-phosphate cytidylyltransferase
Babesia bovis BBOV_I004300   ethanolamine-phosphate cytidylyltransferase, putative
Brugia malayi Bm1_01855   ethanolamine-phosphate cytidylyltransferase
Candida albicans CaO19.2107   Ethanolaminephosphate cytidylyltransferase
Candida albicans CaO19.9655   Ethanolaminephosphate cytidylyltransferase
Caenorhabditis elegans CELE_Y37E3.11   Protein Y37E3.11, isoform B
Cryptosporidium hominis Chro.70332   CTP:ethanolamine cytidylyltransferase
Cryptosporidium parvum cgd7_2950   phospholipid cytidyltransferase HIGH family
Dictyostelium discoideum DDB_G0270298   cytidylyltransferase domain-containing protein
Drosophila melanogaster Dmel_CG5547   Phosphoethanolamine cytidylyltransferase
Echinococcus granulosus EgrG_000163700   Rossmann alpha beta alpha sandwich fold
Entamoeba histolytica EHI_140590   phospholipid cytidylyltransferase, putative
Entamoeba histolytica EHI_095120   phospholipid cytidylyltransferase, putative
Echinococcus multilocularis EmuJ_000163700   Rossmann alpha beta alpha sandwich fold
Homo sapiens 5833   phosphate cytidylyltransferase 2, ethanolamine
Leishmania braziliensis LbrM.32.0980   ethanolamine-phosphate cytidylyltransferase, putative
Leishmania donovani LdBPK_320940.1   ethanolamine-phosphate cytidylyltransferase
Leishmania infantum LinJ.32.0940   ethanolamine-phosphate cytidylyltransferase, putative
Leishmania major LmjF.32.0890   ethanolamine-phosphate cytidylyltransferase, putative
Leishmania mexicana LmxM.31.0890   ethanolamine-phosphate cytidylyltransferase, putative
Loa Loa (eye worm) LOAG_12463   ethanolamine-phosphate cytidylyltransferase
Loa Loa (eye worm) LOAG_15371   hypothetical protein
Mus musculus ENSMUSG00000025137   phosphate cytidylyltransferase 2, ethanolamine
Neospora caninum NCLIV_054370   phosphoethanolamine cytidylyltransferase, putative
Oryza sativa 4349636   Os11g0123400
Oryza sativa 4351359   Os12g0121300
Oryza sativa 4344972   Os08g0224800
Oryza sativa 4348512   Os10g0387000
Plasmodium berghei PBANKA_1360500   ethanolamine-phosphate cytidylyltransferase
Plasmodium falciparum PF3D7_1347700   ethanolamine-phosphate cytidylyltransferase
Plasmodium knowlesi PKNH_1253200   ethanolamine-phosphate cytidylyltransferase, putative
Plasmodium vivax PVX_083280   ethanolamine-phosphate cytidylyltransferase, putative
Plasmodium yoelii PY06073   hypothetical protein
Saccharomyces cerevisiae YGR007W   ethanolamine-phosphate cytidylyltransferase
Schistosoma japonicum Sjp_0030910   ko:K00967 ethanolamine-phosphate cytidylyltransferase [EC2.7.7.14], putative
Schistosoma japonicum Sjp_0030900   ko:K00876 uridine kinase [EC2.7.1.48], putative
Schistosoma mansoni Smp_158130   ethanolamine-phosphate cytidylyltransferase
Schmidtea mediterranea mk4.001631.02  
Trypanosoma brucei gambiense Tbg972.11.15800   ethanolamine-phosphate cytidylyltransferase, putative
Trypanosoma brucei Tb927.11.14140   ethanolamine-phosphate cytidylyltransferase
Trypanosoma congolense TcIL3000_0_31410   ethanolamine-phosphate cytidylyltransferase
Trypanosoma congolense TcIL3000.11.14440   ethanolamine-phosphate cytidylyltransferase
Trypanosoma cruzi TcCLB.511727.120   ethanolamine-phosphate cytidylyltransferase
Toxoplasma gondii TGME49_310280   Phosphoethanolamine-cytidyltransferase
Theileria parva TP04_0187   ethanolamine-phosphate cytidylyltransferase, putative
Trichomonas vaginalis TVAG_327610   ethanolamine-phosphate cytidylyltransferase, putative

Essentiality

LmjF.32.0890 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.5730 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.5730 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.5730 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.5730 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y37E3.11 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y37E3.11 Caenorhabditis elegans larval arrest wormbase
CELE_Y37E3.11 Caenorhabditis elegans slow growth wormbase
PBANKA_1360500 Plasmodium berghei Slow plasmo
TGME49_310280 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier LmjF.32.0890 (Leishmania major), ethanolamine-phosphate cytidylyltransferase, putative
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