Detailed view for Wbm0533

Basic information

TDR Targets ID: 232878
Wolbachia endosymbiont of Brugia malayi, molecular chaperone GrpE (heat shock protein)
Source Database / ID:  GenBank

pI: 7.3239 | Length (AA): 182 | MW (Da): 20973 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01025   GrpE

Gene Ontology

Mouse over links to read term descriptions.
GO:0005739   mitochondrion  
GO:0005515   protein binding  
GO:0000774   adenyl-nucleotide exchange factor activity  
GO:0051087   chaperone binding  
GO:0042803   protein homodimerization activity  
GO:0006457   protein folding  
GO:0030150   protein import into mitochondrial matrix  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
20 180 1dkg (A) 34 195 28.00 0 1 1.1 -0.17
28 179 1dkg (A) 34 194 29.00 0 0.99 1.17 -0.04
28 179 1dkg (A) 34 194 29.00 0 0.99 1.18 -0.04

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126978)

Species Accession Gene Product
Arabidopsis thaliana AT5G55200   co-chaperone GrpE family protein
Arabidopsis thaliana AT4G26780   molecular chaperone GrpE
Arabidopsis thaliana AT5G17710   embryo defective 1241
Babesia bovis BBOV_IV008260   co-chaperone GrpE, putative
Brugia malayi Bm1_03955   GrpE protein homolog, mitochondrial precursor
Candida albicans CaO19.2524   GrpE-like protein similar to S. cerevisiae MGE1 (YOR232W) part of mitochondrial presequence-associated import motor
Candida albicans CaO19.10059   GrpE-like protein similar to S. cerevisiae MGE1 (YOR232W) part of mitochondrial presequence-associated import motor
Caenorhabditis elegans CELE_C34C12.8   Protein C34C12.8
Cryptosporidium hominis Chro.50021   co-chaperone GrpE
Cryptosporidium parvum cgd5_3510   co-chaperone GrpE, putative
Chlamydia trachomatis CT_395   protein GrpE
Dictyostelium discoideum DDB_G0283763   molecular chaperone
Drosophila melanogaster Dmel_CG6155   CG6155 gene product from transcript CG6155-RA
Escherichia coli b2614   heat shock protein
Echinococcus granulosus EgrG_000896100   grpE protein 1 mitochondrial
Echinococcus multilocularis EmuJ_000896100   grpE protein 1, mitochondrial
Homo sapiens 80273   GrpE-like 1, mitochondrial (E. coli)
Leishmania braziliensis LbrM.30.0820   co-chaperone GrpE, putative
Leishmania donovani LdBPK_300760.1   co-chaperone GrpE, putative
Leishmania infantum LinJ.30.0760   co-chaperone GrpE, putative
Leishmania major LmjF.30.0730   co-chaperone GrpE, putative
Leishmania mexicana LmxM.29.0730   co-chaperone GrpE, putative
Loa Loa (eye worm) LOAG_10620   hypothetical protein
Mycobacterium leprae ML2495c   PROBABLE GRPE PROTEIN (HSP-70 COFACTOR)
Mus musculus ENSMUSG00000029198   GrpE-like 1, mitochondrial
Mycobacterium tuberculosis Rv0351   Probable GrpE protein (HSP-70 cofactor)
Mycobacterium ulcerans MUL_0594   GrpE protein (Hsp-70 cofactor)
Neospora caninum NCLIV_040040   GrpE protein homolog, related
Oryza sativa 4345316   Os08g0338700
Oryza sativa 4346621   Os09g0284400
Oryza sativa 4329960   Os02g0612000
Onchocerca volvulus OVOC7884  
Plasmodium berghei PBANKA_0923600   GrpE protein homolog, mitochondrial, putative
Plasmodium falciparum PF3D7_1124700   GrpE protein homolog, mitochondrial, putative
Plasmodium knowlesi PKNH_0922500   GrpE protein homolog, mitochondrial, putative
Plasmodium vivax PVX_091850   GrpE protein homolog, mitochondrial, putative
Plasmodium yoelii PY00817   co-chaperone GrpE, putative
Saccharomyces cerevisiae YOR232W   Mge1p
Schistosoma japonicum Sjp_0030640   ko:K03687 molecular chaperone GrpE, putative
Schistosoma mansoni Smp_062790   grpe protein
Schmidtea mediterranea mk4.000123.07   GrpE protein homolog, mitochondrial
Trypanosoma brucei gambiense Tbg972.6.1940   co-chaperone GrpE, putative
Trypanosoma brucei Tb927.6.2170   co-chaperone GrpE, putative
Trypanosoma congolense TcIL3000_0_31300   co-chaperone GrpE, putative
Trypanosoma cruzi TcCLB.509045.20   co-chaperone GrpE, putative
Trypanosoma cruzi TcCLB.507929.20   co-chaperone GrpE, putative
Toxoplasma gondii TGME49_265220   co-chaperone GrpE protein
Treponema pallidum TP0215   grpE protein (grpE)
Theileria parva TP01_0947   hypothetical protein
Trichomonas vaginalis TVAG_182340   grpe protein, putative
Trichomonas vaginalis TVAG_297650   grpe protein, putative
Wolbachia endosymbiont of Brugia malayi Wbm0533   molecular chaperone GrpE (heat shock protein)

Essentiality

Wbm0533 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.2170 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.2170 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.2170 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.6.2170 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C34C12.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_C34C12.8 Caenorhabditis elegans larval arrest wormbase
CELE_C34C12.8 Caenorhabditis elegans slow growth wormbase
YOR232W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0923600 Plasmodium berghei Essential plasmo
TGME49_265220 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Escherichia coli heat shock protein Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Wbm0533 (Wolbachia endosymbiont of Brugia malayi), molecular chaperone GrpE (heat shock protein)
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