Detailed view for Wbm0254

Basic information

TDR Targets ID: 233091
Wolbachia endosymbiont of Brugia malayi, 3-methyladenine DNA glycosylase
Source Database / ID:  GenBank

pI: 8.0198 | Length (AA): 177 | MW (Da): 20231 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02245   Methylpurine-DNA glycosylase (MPG)

Gene Ontology

Mouse over links to read term descriptions.
GO:0003677   DNA binding  
GO:0003905   alkylbase DNA N-glycosylase activity  
GO:0003824   catalytic activity  
GO:0006284   base-excision repair  

Metabolic Pathways

Base excision repair (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
12 176 1f6o (A) 84 279 35.00 0 1 1.16 -0.62
12 176 1ewn (A) 84 279 35.00 0 1 1.24 -0.3
14 176 1ewn (A) 86 279 36.00 0 1 1.25 -0.45
18 139 1f6o (A) 90 236 43.00 0 1 0.99 0.27

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130143)

Species Accession Gene Product
Arabidopsis thaliana AT3G12040   DNA-3-methyladenine glycosylase
Homo sapiens ENSG00000103152   N-methylpurine-DNA glycosylase
Mycobacterium leprae ML1351   POSSIBLE 3-METHYLADENINE DNA GLYCOSYLASE MPG
Mus musculus ENSMUSG00000020287   N-methylpurine-DNA glycosylase
Mycobacterium tuberculosis Rv1688   Possible 3-methyladenine DNA glycosylase Mpg
Mycobacterium ulcerans MUL_1667   3-methyladenine DNA glycosylase
Oryza sativa 4330890   Os02g0774500
Plasmodium berghei PBANKA_1330400   DNA-3-methyladenine glycosylase, putative
Plasmodium falciparum PF3D7_1467100   DNA-3-methyladenine glycosylase, putative
Plasmodium knowlesi PKNH_1214200   DNA-3-methyladenine glycosylase, putative
Plasmodium vivax PVX_117110   DNA-3-methyladenine glycosylase, putative
Plasmodium yoelii PY05580   putative 3-methyladenine DNA glycosylase
Trichomonas vaginalis TVAG_431800   DNA-3-methyladenine glycosylase, putative
Trichomonas vaginalis TVAG_489150   conserved hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0254   3-methyladenine DNA glycosylase

Essentiality

Wbm0254 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1717 Mycobacterium tuberculosis non-essential nmpdr
PBANKA_1330400 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens N-methylpurine-DNA glycosylase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0226 0.3055 1
0.0181 0.3055 1
0.0266 0.3131 0.9777
0.0256 0.3055 1
0.0264 0.2823 1
0.0252 0.3055 1
0.0251 1 0.5
0.0266 0.3131 0.9777
0.0183 0.2654 1
0.0238 0.2962 0.9217
0.0266 0.3131 0.9777
0.0217 0.2513 1
0.0231 0.3066 1
0.0264 0.2823 1
0.0239 0.3055 1
0.0354865 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Wbm0254 (Wolbachia endosymbiont of Brugia malayi), 3-methyladenine DNA glycosylase
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